• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Molecular diagnosis of pediatric solid tumor

Research Project

Project/Area Number 09670204
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Human pathology
Research InstitutionKeio University

Principal Investigator

UMEZAWA Akihiro  Keio University, School of Medicine, Associate Professor, 医学部・病理学教室, 専任講師 (70213486)

Co-Investigator(Kenkyū-buntansha) KIKUCHI Haruhiro  Keio University, School of Medicine, Instructor, 医学部・中央検査室, 助手 (10161423)
HATA Jun-ichi  Keio University, School of Medicine, Professor, 医学部・病理学教室, 教授 (90051614)
Project Period (FY) 1997 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 1998: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1997: ¥2,000,000 (Direct Cost: ¥2,000,000)
KeywordsEwing sarcoma / E1AF / EWS / chimera gene / translocation / PNET / 小児腫瘍 / 染色体転座 / E1AF
Research Abstract

Many human tumors are now reported to contain characteristic chromosomal translocations. Through the chromosomal translocations, the specific fusion genes are generated. These fusion genes are considered to play an important role in oncogenesis. Ewing' sarcoma and PNET have the same specific chromosomal translocation, t(11 ; 22) and through this translocation, EWS-FLI-1 fusion gene was generated. FLI-1, a partner of the fusion gene, is a member of the Ets family transcription factors. Other members of the Ets family genes, ERG and ETV1, are also fused to EWS through t(21 ; 22) and t(7 ; 22), respectively. Recently, we identified a new fusion gene, EWS-ElAF in a case of Ewing's sarcoma. Since analysis of the fusion genes revealed that Ewing's sarcoma and PNET has the same fusion transcripts, Ewing's sarcoma and PNDT are considered to belong to a common entity. In this report, we extensively characterized a new fusion gene, EWS-ElAF, in detail ; whole cDNA sequence, RNA blot analysis, DNA blot analysis and chromosomal analysis. EWS-ElAF gene is conclusively found to be another fusion gene available for the diagnosis of Ewing's sarcoma and participate in the oncogenesis of Ewing's sarcoma. Furthermore, we have done the genetic analysis of Ewing family of tumors in correlation with immunohistochemical analysis and ultrastructural analysis. From these analyses, several findings were revealed ; limitations of both genetic analysis and histopathological analysis ; relationship between neurogenic phenotypes and chimera genes, diagnosis of Ewing family of tumors from less than 1 mm^3 of biopsy samples. The present findings provide us further significant information concerning the diagnosis and the oncogenesis of Ewing family of tumors.

Report

(3 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • Research Products

    (15 results)

All Other

All Publications (15 results)

  • [Publications] Ishida et al.: "The genetic breakpoint and chimeric transcripts in the EWS-EIAF gene fusion in Ewing sarcoma" Cytogenet and Cell Genet. 82. 278-283 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Urano et al.: "Molecular analysis of Ewing's sarcoma : another fusion gene, EWS-EIAF, available for the diagnosis" Jpn J Cancer Res. 89. 703-711 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Ando et al.: "EAT/mcl-1, a member of the bcl-2 related genes, confers resistance to apoptosis induced by cis-diamine dichloroplatinum(II) via ap53..." Jpn J Cancer Res. 89. 1326-1333 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Ando, T., Umezawa, A., Suzuki, A., Okita, H., Sano, M., Hiraoka, Y., Aiso, S., Saruta, T,and Hata, J.: "EAT/mcl-1, a member of the bcl-2 related genes, confers resistane to apoptosis induced by cis-diamine dichloroplatinum (II) in mouse p53-deficient fibroblast cells." Jpn J Cancer Res. 89(12). 1326-1333 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Ishida, S., Yoshida, K., Kaneko, Y., Tanaka, Y., Sasaki, Y., Urano, F., Umezawa, A., Hata, J-i., and Fujinaga, K.: "The genomic breakpoint and chimeric transcripts in the EWS-E1AF gene fusion in Ewing sarcoma." Cytogenet and Cell Genet. 82. 278-283 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Urano, F., Umezawa, A., Yabe, H., Hong, W., Yoshida, K., Fujinaga, K., and Hata, J-i.: "Molecular analysis of Ewing's sarcoma : another fusion gene, EWS-E1AF,available for the diagnosis." Jpn J Cancer Res. 89. 703-711 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Ando et al.: "EAT/mcC-1,a member of the bcl-2 related genes,confers resistance to apoptosis induced by cis-diamine dichloroplatinum(II)via a p53…" Jpn J Cancer Res. 89. 1326-1333 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Ishida et al.: "The genomic breakpoint and chimeric trauscripts Cu the EWS-E1AF gene fusion in Ewing sarcoma." Cytogenet and Cell Genet. 82. 278-283 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Urano et al.: "Molecular analysis of Ewing's sarcoma:another fusion gene,EWS-E1AF,arailable for the diggnosis." Jpn J Cancer Res.89. 703-711 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Sato,H.et al.: "A new mutation Rim3 resembling Reden is mapped close to retinoic acid alpha(Rara)gene on mouse chromosome 11" Mamm Genome. 9(1). 20-25 (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Neznanor,N.et al.: "A regulatory element within a conding exon modulates Keratin 18 gene expression in transgenic mice" J.Biol.Chem. 272(44). 27549-27557 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Umezawa,A.et al.: "The methylation of an ETs site in the intron enharcer of the Keratin 18 gene participates in tissue specific repression" Mol.Cell.Biol.17(9). 4885-4894 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Hiraoka,Y.et al.: "Isolation and expression of a human SRY-related cDNA,hSOX20." Biochim Biophy Acta. (in press).

    • Related Report
      1997 Annual Research Report
  • [Publications] Sugimoto,J.et al.: "Neurogenic potential of Ewing's sarcoma cells." Virchons Archiv. 430. 41-46 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Rippe,R.et al.: "Binding of upstream stimulatory factor to an E-60x in the 3'-flanking region stimulates alphal(1)collagen gene transcription." J.Biol.Che.272. 1753-1760 (1997)

    • Related Report
      1997 Annual Research Report

URL: 

Published: 1997-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi