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Comparison of Cells Expressed Between Functional Endothelin Receptor Proteins and Their mRNAs by Quantitative Imazing Analyzer System

Research Project

Project/Area Number 09670229
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Experimental pathology
Research InstitutionNagasaki University

Principal Investigator

SHIGEMATSU Kazuto  Nagasaki University School of Medicine, Assistant Professor, 医学部, 助教授 (20154205)

Co-Investigator(Kenkyū-buntansha) NIWA Masami  Nagasaki University School of Medicine, Professor, 医学部, 教授 (20136641)
Project Period (FY) 1997 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 1998: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1997: ¥2,600,000 (Direct Cost: ¥2,600,000)
KeywordsEndothelin / receptor / receptor autoradiography / in situ hybridization / immunohistochemistry / functional receptor / mRNA / in situ hybridigation / endothelin / receptor-autoradiography
Research Abstract

We investigated the distribution of endothelin (ET) receptors and their functions by using our imazing Analyzer system, including quantitative receptor autoradiography, in situ hybridization and Immunohistochemistry. The obtained results were following.
(1) The pattern of ET_A and ET_B ligand binding sites (ftinctional receptor proteins) in the rat brain was classified to 3 fields ; 1) co-localization of both receptors in the circumventricular organs, and anterior and posterior lobes of the pituitary gland, etc., 2) only ET_A receptor in the Rathke pouch of pituitary gland, etc., 3) only ET_B receptor in the external plexiform layer of olfactory bulb and the Purkinje cell layer.
(2) ET_A receptor proteins were observed in the area posterior, the caudal solitary tract nucleus and dorsal nucleus of the vagus in rat brainstem, whereas ET_A mRNA was localized to only area posterior. Therefore, the presence of ET_A neurons projected to the caudal solitary tract nucleus and dorsal nucleus of the vagus from the area posterior may be considered in rat brainstem. On the other hand, the distribution of ET_B receptor proteins was similar to that of ET_B mRNA, and the ET_B receptors were expressed in the glia cells.
(3) The ET_B receptor was capable of binding ET-1 when ET_A receptor was being occupied by BQ-123, ET_A antagonist. Therefore, A collaboration mechanism between the ET_A and the ET_B receptorS may function in the recognition of ET-1, a typical "bivalent" ligand.
(4) The ET_B receptor was expressed in astrocytes, and the possibility the astrocytes can be activated by ET_B receptor in response to neural tissue repair after neuronal death would have to be considered.
(5) The rat peritoneal macrophages apparently express the ET_B receptor but not the ET_A receptor.

Report

(3 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • Research Products

    (18 results)

All Other

All Publications (18 results)

  • [Publications] Shibata S,et al.: "Endothelin receptor in rat pituitary" Cell.Mol.Neurobiol.17. 89-100 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Shibata S,et al.: "The endothelin ET_A receptor exists in the caudal solitary tract nucleus of the rat brain" Cell.Mol.Neurobiol.17. 151-156 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Sakurai-Yamashita Y: "Endothelin receptors in kainic acid-induced neural lesions of rat brain" Neurosciences. 81. 565-577 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Sakurai-Yamashita Y: "Rat peritoneal macrophages express endothelin ET_B but not endothelin ET_A receptors" Eur.J.Pharmacol.338. 199-203 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Himeno A,et al.: "Endothelin-1 binding to endothelin receptors in the rat anterior pituitary gland: Interaction in the recognition--" Cell.Mol.Neurobiol.18. 447-452 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 重松和人他: "脳内エンドセリンとその受容体" CLINICAL NEUROSCIENCE. 16. 1220-1221 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Shibata S, Himeno A Shigematsu K, Tsutsumi K, Sakurai-Yamashita Y, Yamashita K: "Endothelin receptor in rat pituitary gland." Cell Mol Neurobiol. 17. 89-100 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Shibata S, Niwa M, Himeno A, Gana NG, Shigematsu K, Matsumoto M, Yamashita K, Sumikawa K, Taniyama K: "The endothelin ET_A receptor exists in the caudal solitary tract nucleus of the rat brain." Cell Mol Neurobiol.17. 151-156 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Sakurai-Yamashita Y, Niwa M, Yamashita K, Kataoka Y, Himeno A, Shigematsu K, Tsutsumi K, Taniyama K: "Endothelin receptors in kainic acid-induced neural lesions of rat brain." Neuroscience. 81. 565-577 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Sakurai-Yamashita Y, Yamashita K, Yoshida A, Obana M, Takada K, Shibaguchi H, Shigematsu K, Niwa M, Taniyama K: "Rat peritoneal macropharges express endothelin ET_B but not endothelin ET_A receptors" Eur J Pharmacol. 338. 199-203 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Himeno A, Shigematsu K, Taguchi T, Niwa M: "Endothelin-1 binding to endothelin receptors in the rat anterior pituitary gland : Interaction in the recognition of endothelin-1 between ET_A and ET_B receptors." Cell Mol Neurobiol. 18. 447-452 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Shigematsu K, Himeno A, Niwa M: "Endothelins and receptors in the rat brain." CLINICAL NEUROSCIENCE. 16. 1220-1221 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Himeno A,et al.: "Endothelin-1 binding to endothelin receptors in the rat anterion pituitary gland:Interaction in the recognition of endothelin-1 between ETA and ETB receptors" Cell.Mol.Neurobiol.18. 447-452 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 重松和人 他: "脳内エンドセリンとその受容体" Clinical Neuroscience. 16. 1220-1221 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Shibata S: "Endothelin receptors in rat pituitary gland" Cell.Mol.Neurobiol.17. 89-100 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Shibata S: "Endothelin ETA receptor exists in the caudal solitary tract nucleus of the rat brain" Cell.Mol.Neurobiol.17. 151-156 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] S-Yamashita Y: "Endothelin receptors in kainic acid-induced neural lesions of rat brain" Neuroscience. 81. 565-577 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] S-Yamashita Y: "Rat peritoneal macrophages express endothelin ETB but not endothelin ETA receptors" Eur.J.Pharmacol.338. 199-203 (1997)

    • Related Report
      1997 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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