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THE ESTABLISHING OF THE IMMUNE SYSTEM OF THE scid MOUSE BY THE TRANSPLANTATION OF THE XENOGENEIC IMMUNE STEM CELLS

Research Project

Project/Area Number 09670247
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Experimental pathology
Research InstitutionAichi Cancer Center Research Institute

Principal Investigator

TAGUCHI Osamu  AICHI CANCER CENTER RESEARCH INSTITUTE, LABORATORY EXPERIMENTAL PATHOLOGY, SECTION HEAD, 病理学第二部, 室長 (00142167)

Project Period (FY) 1997 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥2,800,000 (Direct Cost: ¥2,800,000)
Fiscal Year 1999: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1998: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1997: ¥1,100,000 (Direct Cost: ¥1,100,000)
KeywordsXENO-GRAFT / IMMUNE STEM CELL / TYMUS / scid MOUSE / NUDE RAT / GVHD
Research Abstract

Because the lymphocyte stem cell has a malfunction, no population of both T and B cells exists in the lymphoid organs of scid (scid/scid) mouse. However the thymus is equipped with the normal organization. Therefore, when transplanting the bone-barrow cells of nude (rnu/rnu) rat to the scid mouse (rat BMT scid mouse), the lymphocytes of the donor origin immigrate into the thymus. Those lymphocytes get to express rat CD3, CD4, CD8 and TCR antigens like the normal rat thymocytes do. The T cell population of the rat origin emerged to the peripheral lymphoid organs, and B cells of rat origin which can produce immunoglobulins emerged there at the same time. These evidences show that the immune system of rat origin can grow sufficiently in the scid mouse. On the other hand, when transplanting the thymic lobes of the 15th of embryonic rats under the kidney capsule of scid mouse, the lobes grow. The immature T cell population which existed in the grafts developed there and emerges to the periphery as the functional T cells. Then, this mouse falls into GVHD after several months of the rat thymus graft and dies. At the rat BMT scid mouse, neither GVHD nor localized autoimmune diseases developed and the long-range survival is possible. We gave the rat BMT scid mouse a foreign antigen (SRBC) and examined immune function but the reaction was very weak. This phenomenon is peculiar, because both the expression patterns of TCR of T cells and IgG of B cells in rat BMT scid mouse are as those of normal rat. When injecting the spleen cells of the rat BMT scid mouse into nude rat, the decrease of the weight is remarkable from 3 months to 5 months after the treatment and the GVHD-like disease occurred. The rat BMT scid mouse is the model which is extremely useful to study education effects to the T cell of the thymus.

Report

(4 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • 1997 Annual Research Report
  • Research Products

    (34 results)

All Other

All Publications (34 results)

  • [Publications] Doi, T. et al.: "NF-κB relA deficient lymphocytes : Normal developmen of T cells and B cells, impaired production of IgA and IgG1 and reduced proliferative responses"J. Exp. Med.. 185. 953-961 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Igakura, T. et al.: "A null mutation in basigin, an immunoglobulin superfamily mumber, indicates its importans roles in peri-implantation development and spermatogenesis"Dev. Biol.. 194. 152-165 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Takeuchi, M. et al.: "Suppression of spontaneous uveoretinitis development by non-immunopathogenic peptide immunization"Eur. J. Immunol.. 28. 1578-1586 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Kagami, Y. et al.: "Establishment of an IL-2-dependent cell line derived from 'nasal-type'NK/T-cell lymphoma of CD2+, sCD3-,CD3_ε+, CD56+ phenotype and associated with the Epstein-Barr virus"Brit. J. Haematol.. 103. 669-677 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Ohno, K. et al.: "Successful transfer of localized autoimmunity with positively selected CD4^+ cells to scid mice lacking functional B cells"Autoimmunity. 29. 103-110 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Yasumura, M. et al.: "Protective effects of 5, 6, 7, 8-tetrahydroneopterin against x-ray radiation injury in mice"Biochim. Biophys. Acta.. 1453. 378-384 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Ishiguro, K. et al.: "Syndecan-4 expression is associated with follicular atresia in mouse ovary"Histochem. Cell. Biol.. 112. 25-33 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Nishigaki-Maki, K. et al.: "Autoimmune diseases developed in athymic nude mice grafted with embryonic thymus of xenogeneic origin"Eur.J.Immunol.. 29. 3350-3359 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Hayashi, Y. et al.: "A binding site for transcription factor Grainyhead/NTF-1 contributes to the regulation of the Drosophila proliferation cell nuclear antigen gene promoter"J. Biol. Chem.. 274. 35080-35088 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Morimoto, T. et al.: "Transplatation of xenogeneic embryonic thymus to athymic nude mice induces acquisition of distorted immunity"Transpl. Proc.. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Doi, T., Takahashi, T., Taguchi, O., Azuma, T. and Obata, Y.: "NF-ィイD2kィエD2B relA deficient lymphocytes: Normal development of T cells and B cells, impaired production of IgA and IgG1 and Reduced proliferative responses."J. Exp. Med.. 185. 953-961 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Igakura, T., Katomatsu, K., Kaname, T., Muramatsu, H., Fan, Q.-W., Miyaushi, T., Toyama, Y., Kuno, N., Yuasa, S., Takahashi, M., Senda, T., Taguchi, O., Yamamura, K., Arimura, K. and Muramatsu, T.: "A null mutation in basigin, an immunoglobulin superfamily member, indicates its important roles in peri-implantation development and spermatogenesis."Dev. Biol.. 194. 152-165 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Takeuchhi, M., Kezuka, T., Inoue, H., Sakai, J., Usui, M., Takahashi, T. and Taguchi, O.: "Suppression of spontaneous uveroretinitis development by non-immunopathogenic peptide immunization."Eur. J. Immunol.. 28. 1578-1586 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Kagami, Y., Nakamura, S., Suzuki, R., Iida, S., Yatabe, Y., Okada, Y., Kobayashi, T., Tsurumi, T., Seto, M., Ogura, M., Taguchi, O. and Morishima, Y.: "Establishment of an IL-2-dependent cell line derived from 'nasal-type' NK/T-cell lymphoma of CD2+, sCD3-, CD3ε+, CD56+ phenotype and associated with the Epstein-Barr virus."Brit. J. Haematol.. 103. 669-677 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Ohno, K., Takahashi, T., Maki, K., Ueda, M. and Taguchi, O.: "Successful transfer of localized autoimmunity with positively selected CD4ィイD1+ィエD1 cells to scid mice lacking functional B cells."Autoimmunity. 29. 103-110 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Yasumura, M., Morimoto, T., Takagi, M., Hirose, H. and Taguchi, O.: "Protective effects of 5,6,7,8-tetraphydroneopterin against x-ray radiation injury in mice."Biochim. Biophys. Acta.. 1453. 378-384 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Ishiguro, K., Kojima, T., Taguchi, O., Saito, H., Muramatsu, T. and Kadomatsu, K.: "Syndecan-4 expression is associated with follicular atresia in mouse ovary."Histochem. Cell. Biol.. 112. 25-33 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Nishigaki-Maki, K., Takahashi, T., Ohno, K., Morimoto, T., Ikeda, H., Takeuchi, M., Ueda, M. and Taguchi, O.: "Autoimmune diseases developed in athymic nude mice grafted with embryonic thymus opf xenogeneic origin."Eur. J. Immunol.. 29. 3350-3359 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Hayashi, Y., Yamagishi, M., Nishimoto, Y., Taguchi, O., Matsukage, A. and Yamaguchi, M.: "A binding site for transcription factor Grainyhead/NTF-1 contributes to the regulation of the Drosophila proliferating cell nuclear antigen gene promoter."J. Biol. Chem.. 274. 35080-35088 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Morimoto, T., Nishigaki-Maki, K., Ohno, K., Ikeda, H., Takeuchi, M., Ueda, M., Takahashi, T. and Taguchi, O.: "Transplantation of xenogeneic embryonic thymus to athylmic nude mice induces acquisition of distorted immunity."Transp1. Proc.. (In press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Ohno, K. et al: "Successful transfer of localized autoimmunity with positively selected CD4^+ cells to scid mice lacking functional B cells"Autoimmunity. 29. 103-110 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Yasumura, M. et al: "Protective effects of 5, 6, 7, 8-tetrahydroneopterin against x-ray radiation injury in mice"Biochim. Biophys. Acta.. 1453. 378-384 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Ishiguro, K. et al: "Syndecan-4 expression is associated with follicular atresia in mouse ovary"Histochem. Cell. Biol.. 112. 25-33 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Nishigaki-Maki, K. et al: "Autoimmune diseases developed in athymic nude mice grafted with embryonic thymus of xenogeneic origin"Eur. J. Immunol.. 29. 3350-3359 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Hayashi, Y. et al: "A binding site for transcription factor Grainyhead/NTF-1 contributes to the regulation of the Drosophila proliferating cell nuclear antigen gene promoter"J. Biol. Chem.. 274. 35080-35088 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Morimoto, T. et al: "Transplantation of xenogeneic embryonic thymus to athymic nude mice induces acquisition of distorted immunity"Transpl. Proc.. (In press).

    • Related Report
      1999 Annual Research Report
  • [Publications] Igakura,T.et al: "A null mutation in basigin,an immunoglobulin superfamily member,indicates its important roles in peri-implantation development and spermataogenesis" Dev.Biol.194. 152-165 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Takeuchi,M.et al: "Suppression of spontaneous uveoretinitis development by non-immunopathogenic peptide immunization" Eur.J.Immunol.28. 1578-1586 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Ohno,K.et al: "Successful transfer of localized autoimmunity with positively selected CD4+cells to scid mice lacking functional B cells" Autoimmunity. (in press).

    • Related Report
      1998 Annual Research Report
  • [Publications] Yasumura,M.et al: "Protective effects of 5,6,7,8-tetrahydroneopterin against X-ray radiation injury in mice" Biochim.Biophys.Act. (in press).

    • Related Report
      1998 Annual Research Report
  • [Publications] Doi,T.et al.: "NF-κ B relA deficient lymphocytes:Normal development of T cells and B cells,impaired production of IgA and IgGl and reduced proliferative responses." Journal of Experimental Medicine. 185. 953-961 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Igakura,T.et al.: "A null mutation in basigin,an immunoglobulin superfamily member,indicates its important roles in peri-implantation development and spermatogenesis." Developmental Biology. (in press).

    • Related Report
      1997 Annual Research Report
  • [Publications] Takeuchi,M.et al.: "Suppression of spontaneous uveoretinitis development by non-immunopathogenic peptide immunization." European Journal of Immunology. (in press).

    • Related Report
      1997 Annual Research Report
  • [Publications] Ohno,K.et al.: "Successful transfer of localized autoimmunity with positively selected CD4+ cells to scid mice lacking functional B cells." Autimmunity. (in press).

    • Related Report
      1997 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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