| Project/Area Number |
09670255
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
寄生虫学(含医用動物学)
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| Research Institution | Gifu University School of Medicine |
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Principal Investigator |
TAKAHASHI Yuzo Department of Parasitology, Gifu University School of Medicine, Professor, 医学部, 教授 (80094580)
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| Co-Investigator(Kenkyū-buntansha) |
NAGANO Isao Department of Parasitology, Gifu University School of Medicine, ProfessorGifu Un, 医学部, 講師 (40283296)
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| Project Period (FY) |
1997 – 1998
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| Project Status |
Completed (Fiscal Year 1998)
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| Budget Amount *help |
¥2,900,000 (Direct Cost: ¥2,900,000)
Fiscal Year 1998: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 1997: ¥1,400,000 (Direct Cost: ¥1,400,000)
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| Keywords | Trichinella / ES products / stichosome / peptide / 筋肉細胞 / 分化 |
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| Research Abstract |
Mass and pure production of peptide using cDNA library of Trichinella was achieved for the two reasons. One is to obtain antigen for immunodiagnostic purposes and/or immunoresponse analysis. The other is to obtain and analysis protein that may alter gene expression of host muscle cells, known as nurse cell formation. We have constructed cDNA library of T spiralis and T pseudospiralis, and produced some of possibly important peptides. The method includes isolation of mRNA from muscle larvae, cDNA library construction, immunoscreening of E coli colony against infected sera. Ten-positive clones were obtained, 5 out of which were sequenced. Homology analysis showed Clone 111 has homology to protenase inhibitor, and Clone 132 seemed to be identical to one reported by Despommier. Other clones were unique showing no similarity to previously reported sequences. Western blot analysis of synthesized peptides reacted against anti ES antibody or infected sera, or failed to react.
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