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Role of the rhoptry proteins of sporozoites during hepatocyte invasion by Plasmodium

Research Project

Project/Area Number 09670262
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 寄生虫学(含医用動物学)
Research InstitutionEhime University

Principal Investigator

TORII Motomi  Ehime University School of Medicine, Department of Parasitology, Professor, 医学部, 教授 (20164072)

Co-Investigator(Kenkyū-buntansha) TSUBOI Takafumi  Ehime University School of Medicine, Department of Parasitology, Associate Profe, 医学部, 助教授 (00188616)
Project Period (FY) 1997 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥2,700,000 (Direct Cost: ¥2,700,000)
Fiscal Year 1998: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1997: ¥1,700,000 (Direct Cost: ¥1,700,000)
Keywordsmalaria / sporozoite / plasmodium berghei / rhoptry / ロプトリ-
Research Abstract

All invasive forms of Apicomplexa possess a complex of organelles located at the anterior end of the parasite. The organelles comprise rhoptries, dense granules and micronemes. The rhoptries are pear shaped, electron-dense, membrane bound organdies and are considered to have roles in host cell attachment, invasion and formation of parasitophorous vacuole membrane (PVM). Membranous materials are secreted from the rhoptries during the invasion. These materials are thought to be incorporated into the expanding PVM.Several proteins of varying molecular weight have been identified in P.falciparum merozoite rhoptries, but apical organelles of sporozoite have not been investigated. To analyze the rhoptry proteins of sporozoites, we try to produce monoclonal antibodies against sporozoite rhoptry proteins of rodent malaria. P.berghei sporozoites were enriched and were disrupted by nitrogen decompression method in order to release the organelles in a mild condition. Organelles were then, purified by sucrose density gradient centrifugation. Subcellular fractions were observed by the electron microscopy. We immunized BALB/c mice with these fractions or disrupted native sporozoites. We are trying to clone the hybridoma which produce the monoclonal antibody against P.berghei sporozoite apical organelles by using ELISA or IFA test.

Report

(3 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • Research Products

    (10 results)

All Other

All Publications (10 results)

  • [Publications] Y-M.Cao et al.: "Nitric Oxide inhibits the development of Plasmodium yoelii gametocytes into gametes" Parasitol.Int.47(2). 157-166 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Y-M.Cao et al.: "Infected host serum blocks transmission of Plasmodium yoelii via a nitric oxide-dependent mechanism" Parasitol.Int.47(3). 225-232 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] T.Tsuboi et al.: "Sequence pclymorphism in two novel Plasmodium vivax ookinete surface proteins,Pvs25 and Pvs28,that are malaria transinission-blocking vaccine candidates" Mol.Med.(In Press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Y-M.Cao, T.Tsuboi, M.Torii: "Nitric oxide inhibits the development of Plasmodium yoelii gametocytes into gametes." Parasitol. Int.47 (2). 157-166 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Y-M.Cao, T.Tsuboi, Y-J.Liu, M.Torii: "Infected host serum blocks transmission of Plasmodium yoelii via a nitric oxide-dependent mechanism." Parasitol. Int.47 (3). 225-232 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] T.Tsuboi, D.C.Kaslow, M.M.G.Gozar, M.Tachibana, Y-M.Cao, M.Torii: "Sequence polymorphism in two novel Plasmodium vivax ookinets surface proteins, Pvs25 and Pvs28, that are malaria transmission-blocking vaccine candidates." Mol.Med.(In press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Y-M.Cao et al.: "Nitric oxide inhibits the development of Plasmodium yoelii gametocytes into gametes" Parasitol.Int.47(2). 157-166 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Y-M.Cao et al.: "Infected host serum blocks transmission of Plosmodium yoelii via a nitric oxide-dependent mechanism." Parasitol.Int.47(3). 225-232 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] T.Tsuboi et al.: "Sequence polymorphism in two novel Plasmodium vivax ookinete surface proteins, Pvs25 and Pvs28, that are malaria transmission-blocking vaccine candidates." Mol.Med.In Press.

    • Related Report
      1998 Annual Research Report
  • [Publications] Ya-Ming Cao et al.: "Nitric oxide inhibits the development of Plasmodium yoelii gametocytes in to gametes." Parasitology International. 47(in press). (1998)

    • Related Report
      1997 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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