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Localization of host sequences in parasitic schistosomes

Research Project

Project/Area Number 09670270
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 寄生虫学(含医用動物学)
Research InstitutionIbaraki Prefectural University of Health Sciences

Principal Investigator

IWAMURA Yukio  Ibaraki Prefectural University of Health Sciences, Prof., 保健医療学部, 教授 (90110486)

Project Period (FY) 1997 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1999: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1998: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1997: ¥1,000,000 (Direct Cost: ¥1,000,000)
Keywordsin situ PCR / Schistosoma mansoni / Schistosoma japonicum / type 2 Alu / IAP / env / MHC / B2 / enV / ミトコンドリアDNA
Research Abstract

DNA sequences homologous to repetitive sequences in the host genome such as mouse type 1 Alu sequence (B1), type 2 (B2), the mouse retrovirus related sequences such as mouse intracisternal A-particle (IAP) and endogenous type C retrovirus sequence detected in the DNA of schistosomes. Furthermore, sequences of the mouse major histocompatibility complex (MHC) were also detected in the DNA of schistosomes. Localization of these sequences were examined by in situ polymerase chain reaction (in situ PCR) in schistosomes. The signals were detected in the cytoplasm of the tegumental membrane and in the nuclei of the mesenchymal, testicular, ovarian and vitelline cells of 8-week Schistosoma japonicum. In contrast, it was difficult to detect any signals of this sequence in 8-week S. mansoni, whereas in 24-week male S. mansoni the signals were observed in the cytoplasm of the tegumental tubercles and in the nuclei of the mesenchymal and testicular cells. On the other hand, in 24-week female S.mansoni the signals were found in the nuclei of the mesenchymal, ovarian and vitelline cells but not found in the tegument. Moreover, sequences of IAP and MHC were detected in the cercarial DNA of schistosomes. These nucleotide sequences were the same as those of the host (mouse).

Report

(4 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • 1997 Annual Research Report

Research Products

(30 results)

All Other

All Publications (30 results)

  • [Publications] Atsuko Imase: "Horizonatla and vertical transfer of mouse endogenous retoroviral DNA sequences in schistosomes"Parasitology. (in press). (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Atsuki Imase: "Localization of mouse type 2 Alu sequence in Schistosomes"Parasitology. 119. 315-322 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Yasutomo Sakai: "Acute exercise causes mitochondrial DNA deletion in rat skeletal muscle"muscle & Nerve. 22. 258-261 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Kotoyo Isobe: "Nuclear-recessive mutations of factors involved in mitochondrial translation ore responsible for age-related respiration deficiency of human skin fibroblasts"Journal of biological Chemistry. 273. 4601-4606 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Nobuo Kubota: "Induction of particular deletion in mitochondrial DNA by X rays depends on the inherent radiosensitivity of the cells"Radiation Research. 148. 395-398 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Yasutomo Sakai: "Hypokinesia causes oxidative damage and mitochondrial DNA deletion in rat skeletal muscle"Journal of Physicak Therapy Science. 9. 103-109 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] IMASE,Atsuko, et al.: "Horizontal and vertical transfer of mouse endogenous retroviral DNA sequences in schistosomes"Parasitology. in press. (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] IMASE,Atsuko, et al.: "Localization of mouse type 2 Alu sequences in schistosomes"Parasitology. 119(3). 315-322 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] SAKAI,Yasutomo, et al.: "Acute exercise causes mitochondrial DNA deletion in rat skeletal muscle"Muscle & Nerve. 22(2). 258-261 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] ISOBE,Kotoyo, et al.: "Nuclear-recessive mutations of factors involved in mitochondrial translation are responsible for age-related respiration deficiency of human skin fibroblasts"Journal of Biological Chemistry. 273(8). 4601-4606 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] KUBOTA,Nobuo, et al.: "Induction of a particular deletion in mitochondrial DNA by X rays depends on the inherent radiosensitivity of the cells"Radiation Research. 148(4). 395-398 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] SAKAI,Yasutomo, et al.: "Hypokinesia causes oxidative damage and mitochondrial DNA deletion in rat skeletal muscle"Journal of Physical Therapy Science. 9(1). 103-109 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Atsuko Imase: "Horizontal and vertical transfer of mouse endogenous retorovial DNA sequences in schistosomes"Parasitology. (in press). (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] Atsuko Imase: "Localization of mouse type 2 Alu sequence in Schistosomes"Parasitology. 119・3. 315-322 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Yasutomo Sakai: "Acute exercise causes mitochondrial DNA deletion in rat skeletal muscle"Muscle & Nerve. 22・2. 258-261 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Kotoya Isobe: "Nuclear-recessive mutations of factors involved in mitochondrial translation are responsible for age-related respiration deficiency of human skin fibroblasts"Journal of Biological Chemistry. 273・8. 4601-4606 (1998)

    • Related Report
      1999 Annual Research Report
  • [Publications] Nobuo Kubota: "Induction of a particular deletion in mitochondrial DNA by X rays depends on the inherent radiosensitivity of the cells"Radiation Research. 148・4. 395-398 (1997)

    • Related Report
      1999 Annual Research Report
  • [Publications] Yasutomo Sakai: "Hypokinesia causes oxidative damage and mitochondrial DNA deletion in rat skeletal muscle"Journal of Physical Therapy Science. 9・1. 103-109 (1997)

    • Related Report
      1999 Annual Research Report
  • [Publications] Yasutomo Sakai: "Acute exercise causes mitochondrial DNA deletion in rat skeletal muscle" Muscle & Nerve. 22・2. 258-261 (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] 山本和央: "PCR-SSCP分析法を用いたCharcot-Marie-Tooth病の遺伝子異常スクリーニング" 茨城県立医療大学紀要. 3巻. 67-72 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Kotoyo Isobe: "Nuclear-recessive mutations of factors involved in mitochondrial translation are responsible for age-related respiration deficiency of human skin fibroblasts" Journal of Biological Chemistry. 273・8. 4601-4606 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Nobuko Kubota: "Induction of a particular deletion in mitochondrial DNA by X rays depends on the inherent radiosensitivity of the cells" Radiation Research. 148・4. 395-398 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] 今瀬敦子: "In situ PCR法を用いたマウス肝臓組織標本上での高度反復配列B2の検出" 茨城県立医療大学紀要. 2巻. 5-9 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] Atsuko Imase: "Abnormal Structure and Expression of the K-rev Gene in Neuroblastoma Cell Lines" ASVPI. 2. 1-4 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] Yasutomo Sakai: "Hypokinesia causes oxidative damage and mitochondrial DNA deletion in rat skeletal muscle" Journal of Physical Therapy Science. 9. 103-109 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] Kotoyo Isobe: "Nuclear-recessive mutations of factors involved in mitochondrial translation are responsible for age-related respiration deficiency of human skin fibroblasts" Journal of Biological Chemistry. 273・8. 4601-4606 (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Nobuo Kubota: "Induction of a particular deletion in mitochondrial DNA by X rays depends on the inherent radiosensitivity of the cells" Radiation Research. 148・4. 395-398 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 今瀬敦子: "In situ PCR法を用いたマウス肝臓組織標本上での高度反復配列B2の検出" 茨城県立医療大学紀要. 2巻. 5-9 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Atsuko Imase: "Abnormal Structure and Expression of the K-rev Gene in Neuroblastoma Cell Lines" ASVPI. 2. 1-4 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Yasutomo Sakai: "Hypokinesia causes oxidative damage and mitochondrial DNA deletion in rat skeletal muscle" Journal of Physical Therapy Science. 9(in press). (1997)

    • Related Report
      1997 Annual Research Report

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Published: 1997-03-31   Modified: 2016-04-21  

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