Mechanism of growth stimulatory effect of vascular endothelial cells by Bartonella henselae.
| Project/Area Number |
09670290
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Bacteriology (including Mycology)
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| Research Institution | Kagoshima University |
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Principal Investigator |
ODA Hiroshi Faculty of Medicine Kagoshima University, Professor, 医学部, 教授 (40107868)
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| Co-Investigator(Kenkyū-buntansha) |
MAENO Nobuaki Faculty of Medicine Kagoshima University, Research Associate, 医学部, 助手 (20305113)
YOSHIIE Kiyotaka Faculty of Medicine Kagoshima University, Associate Professor, 医学部, 助教授 (70174886)
モハマッド レズワヌル・ 鹿児島大学, 医学部, 助手 (60284866)
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| Project Period (FY) |
1997 – 1998
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| Project Status |
Completed (Fiscal Year 1998)
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| Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1998: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1997: ¥1,700,000 (Direct Cost: ¥1,700,000)
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| Keywords | Bartonella henselae / Bartonella infection / bacillary angiomatosis / endothelial cells / growth factor / angiogenesis / Richettsia / angiogenic factor / angiomatosis |
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| Research Abstract |
We studied on the mechanism of growth stimulatory effect of vascular endothelial cells by Bartonella henselae, and obtained the following results ;
1. Interaction between B, henselae and human umbilical vein endothelial cells (HUVECs) : B.henselae ATCC 49882 (10^7 CFU/ml) was cocultivated with HUVECs, and the growth of HUVECs was estimated by MTT assay. The proliferation of HUVECs cocultivated with live B.henselae was enhanced in inoculated-bacterial dose dependent manner, and the stimulatory effect was specific for vascular endothelial cells. Similar growth enhancement effect was also observed in endothelial cells from rabbits or dogs.
2. Active component and mechanism of growth enhancement : B.henselae killed by UV irradiation or heat treatment abolished its stimulatory activity, suggesting that the effect was exhibited by some action of live bacteria, not only by the existence of bacterial cells. To investigate the role of direct contact, live B.henselae were separated from HUVECs by membrane filter (Millicell-CM). Even under this condition, the HUVECs proliferation stimulating effect was observed. However, the morphological changes of HUVECs were not apparent compared to B.henselae infected cells. Furthermore, we isolated a nonpiliated B.henselae strain which was unable to attach to and entry into endothelial cells. The nonpiliated strain possessed the ability to stimulate the proliferation of HUVECs same as piliated strain. Our results suggested that B.henselae might produce some angiogenic factor(s) and secrete it. Further study is currently under way to purify and characterize this angiogenic factor.
3. In vivo study : B.henselae was inoculated into chorioallantoic membrane of embryonated eggs, mice and guinea pigs (subcutaneous or intradermal routs), however, no angiogenic lesion was observed in the animals.
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Report
(3 results)
Research Products
(3 results)
