| Project/Area Number |
09670421
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Public health/Health science
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| Research Institution | Kobe Gakuin University |
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Principal Investigator |
OKAMOTO Tadashi Kobe Gakuin University, Faculty of Pharmaceutical Sciences, Lecturer, 薬学部, 講師 (80194398)
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| Co-Investigator(Kenkyū-buntansha) |
TAKAHASHI Takayuki Kobe Gakuin University, Faculty of Pharmaceutical Sciences, Research Assistant, 薬学部, 助手 (40216726)
KISHI Takeo Kobe Gakuin University, Faculty of Pharmaceutical Sciences, Professor, 薬学部, 教授 (70028843)
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| Project Period (FY) |
1997 – 1998
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| Project Status |
Completed (Fiscal Year 1998)
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| Budget Amount *help |
¥2,400,000 (Direct Cost: ¥2,400,000)
Fiscal Year 1998: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1997: ¥1,400,000 (Direct Cost: ¥1,400,000)
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| Keywords | Ubiquinone / Maximal oxygen uptake / Cardiac myocytes / Antioxidants / Oxidative stress / Selenium / glutathione peroxidase / Ergometer / heart / selenium / glutathione / cultured cardiac myocytes / γ-glutamylcysteinylethyl ester / cell injury / oxidative stress / ubiquinone / coenzyme Q / 骨格筋 / 酸化的ストレス / 運動負荷 / 血清 |
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| Research Abstract |
1)Decreased serum ubiquinol-lO levels in healthy subjects during exercise at maximal oxygen uptake
We studied the changes of serum ubiquinol-lO (UQH_2-1O, a reduced form of ubiquinone) levels during exercise on a bicycle ergometer at maximal oxygen uptake (VO_<max>) In response to the exercise, serum level of lactate at VO_<max> was S times higher than that before the exercise. Among serum antioxidants, the ratio of the UQH_2-1O to the total UQ-1O (UQH_2-1O/total UQ-1O (UQH_2-1O + UQ-1O)) at VO_<max> was significantly lower than that before the exercise. Serum levels of ascorbate but not alpha-tocopherol at VO_<max> were 1.2 times higher than those before the exercise.
This study demonstrated that in the response to aerobic exercise on a bicycle ergometer at VO_<max>, serum levels of UQH_2-1O and ascorbate changed, and also showed that such antioxidants may play beneficial role for the elimination of exercise-derived oxygen radicals.
2)Protective effect of gamma-glutamylcysteinylethyl ester on dysfunction of the selenium-deficient rat heart
Selenium (Se) deficiency induced a 62% incidence of electrocardiographic abnormalities, a 63% reduction in dP/dt in the left ventricle, and an increase in thiobarbituric acid reacting substances (TBARS). Administration of gamma-glutamylcysteinylethyl ester (gamma-GCE)(a membrane-permeating GSH precursor) increased the intracellular GSH concentration ([GSH]i) and prevented the cardiac dysfunction and the TBARS increment. The V*, but not the K_m, for GSH of Se-dependent GSH peroxidase (Se-Gpx) activity in Se-deficient rat heart homogenates was one-third that of normal rat heart homogenates. Although gamma -GCE did not affect the Se-Gpx V_<max> and K_m, forGSH, it did induce a substantial and significant increase in [GSH]_i.
These data suggest that the increase in [GSH]_i may have played a role in preventing the TBARS increase and cardiac dysfunction in Se-deficient rats.
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