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Development of Forensic Diagnosis of Acture Sarin Poisoning

Research Project

Project/Area Number 09670431
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Legal medicine
Research InstitutionThe University of Tokyo

Principal Investigator

NAGAO Masataka  Graduate School of Medicine, Department of Forensic Medicine, Associate Professor, 大学院・医学系研究科, 助教授 (80227991)

Co-Investigator(Kenkyū-buntansha) TAKATORI Takehiko  Graduate School of Medicine, Department of Forensic Medicine, Associate Professo, 大学院・医学系研究科, 教授 (30001928)
Project Period (FY) 1997 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 1998: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 1997: ¥2,400,000 (Direct Cost: ¥2,400,000)
KeywordsSarin / Acethylcholinesterase / Erythocyte / Carebellum / Aikaline Phosphatase / Isopropyl methylphosphonic acid / Methylpgosphonic acid / GC-MS / Sarin / Acthyl cholinesterase / Cerebellum / Alkaline Phosphatase / methylphosphoric acid / Acthylcholinesterase / Erythrocyte / isopropyl methylphosphonic acid / methylphosphonic acid
Research Abstract

A sarin-like organophosphorus agent, bis (isopropyl methyl) phosphonate (BIMP), was synthesized. This agent has the same phosphonate group as sarin and also has the same anti-acethylcholinesterase activity potency as sarin. After preparation of experimental BIMP-exposed human red blood cells, BIMP-bound acetylcholinesterase (AChE) was solubilized from erythrocyte membranes, purified by immunoaffinity chromatography, digested with trypsin, and the sarin hydrolysis products bound to AChE were released by alkaline phosphatasedigestion. The digested sarin hydrolysis products were subjected to trimethylsilyl (TMS) derivatization and detected by gas chromatography-mass spectrometry (GC-MS). Isopropyl methylphosphonic and methylphosphonic acids, which are the sarin hydrolysis products, were detected in experimental BIMP-exposed human red blood cells. Using this new method, we could detected the sarin hydrolysis products from erythrocytes and formalin-fixed cerebellums of victims of the Tokyo subway terrorist attack.

Report

(3 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report

Research Products

(9 results)

All Other

All Publications (9 results)

  • [Publications] Masataka Nagao et al.: "Detection of Sarin Hydrolysis Products from Sarin-like Organophosphorus Ogent-Exposed Human Erythrocytes" Journal of Chromatography Biomedical Applications. 701・1. 9-17 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Masataka Nagao et al.: "Definitive Evidence for the Acute Sarin Poisoning Diagnosis in the Tokyo Subway" Toxicology and Applied Pharmacology. 144・1. 198-203 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Yukimasa Matsuda et al.: "Detection of Sarin Hydrolysis Product in the Formalin-fixed Brain Tissue of Victims of the Tokyo Subway Terrorist Attack" Toxicology and Applied Pharmacology. 150・2. 310-320 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Nagao M et al.: "Detrection of sarin hydrolysis products from sarin-like organo-phosphorus agent-exposed human erythrocytes." J Chromatogr B. 701 (1). 9-17 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Nagao M et al.: "Definitive evidence for the acute sarin poisoning diagnosis in the Tokyo subway." Toxicol Appl Pharmacol. 144 (1). 198-203 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Matsuda Y et al.: "Detrection of sarin hydrolysis product in formalin-fixed brain tissues of victims of the Tokyo Subway Terrorist Attack." Toxicol Appl Pharmacol. 150 (2). 310-20 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Yukimasa Matsuda et al.: "Detection of the Sarin Hydrolysis Product in the Formalin-Fixed Brain Tissue of Victims of the Tokyo Subway Terrorist Attack" Toxicology and Applied Pharmacology. 150・2. 310-320 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Masataka Nagano et al.: "Detection of sarin hydrolysis proclucts from sarin-like organo phosphorus agent-exposed human erythrocytes" Journal of Chromatography Biomedical Applications. 701・1. 9-17 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Masatka Nagano et al.: "Definitive Evidence for the Acute Sarin Poisoning Diagnosis in the Tokyo Subway" Toxicology and Applied pharmacology. 144・1. 198-203 (1997)

    • Related Report
      1997 Annual Research Report

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Published: 1997-03-31   Modified: 2016-04-21  

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