| Project/Area Number |
09670431
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Legal medicine
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| Research Institution | The University of Tokyo |
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Principal Investigator |
NAGAO Masataka Graduate School of Medicine, Department of Forensic Medicine, Associate Professor, 大学院・医学系研究科, 助教授 (80227991)
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| Co-Investigator(Kenkyū-buntansha) |
TAKATORI Takehiko Graduate School of Medicine, Department of Forensic Medicine, Associate Professo, 大学院・医学系研究科, 教授 (30001928)
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| Project Period (FY) |
1997 – 1998
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| Project Status |
Completed (Fiscal Year 1998)
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| Budget Amount *help |
¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 1998: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 1997: ¥2,400,000 (Direct Cost: ¥2,400,000)
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| Keywords | Sarin / Acethylcholinesterase / Erythocyte / Carebellum / Aikaline Phosphatase / Isopropyl methylphosphonic acid / Methylpgosphonic acid / GC-MS / Sarin / Acthyl cholinesterase / Cerebellum / Alkaline Phosphatase / methylphosphoric acid / Acthylcholinesterase / Erythrocyte / isopropyl methylphosphonic acid / methylphosphonic acid |
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| Research Abstract |
A sarin-like organophosphorus agent, bis (isopropyl methyl) phosphonate (BIMP), was synthesized. This agent has the same phosphonate group as sarin and also has the same anti-acethylcholinesterase activity potency as sarin. After preparation of experimental BIMP-exposed human red blood cells, BIMP-bound acetylcholinesterase (AChE) was solubilized from erythrocyte membranes, purified by immunoaffinity chromatography, digested with trypsin, and the sarin hydrolysis products bound to AChE were released by alkaline phosphatasedigestion. The digested sarin hydrolysis products were subjected to trimethylsilyl (TMS) derivatization and detected by gas chromatography-mass spectrometry (GC-MS). Isopropyl methylphosphonic and methylphosphonic acids, which are the sarin hydrolysis products, were detected in experimental BIMP-exposed human red blood cells. Using this new method, we could detected the sarin hydrolysis products from erythrocytes and formalin-fixed cerebellums of victims of the Tokyo subway terrorist attack.
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