| Project/Area Number |
09670437
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Legal medicine
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| Research Institution | Mie University |
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Principal Investigator |
FUKUNAGA Tatsushige Mie University, Faculty of Medicine, Professor, 医学部, 教授 (70156800)
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| Co-Investigator(Kenkyū-buntansha) |
YAMAMOTO Hidetaka Mie University, Faculty of Medicine, Assistant, 医学部, 助手 (90158296)
TANEGASHIMA Akio Mie University, Faculty of Medicine, Lecture, 医学部, 講師 (70283520)
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| Project Period (FY) |
1997 – 1998
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| Project Status |
Completed (Fiscal Year 1998)
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| Budget Amount *help |
¥2,900,000 (Direct Cost: ¥2,900,000)
Fiscal Year 1998: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1997: ¥1,700,000 (Direct Cost: ¥1,700,000)
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| Keywords | Alcohol, ethyl / Acetaldehyde / ALDH / ADH / cDNA / mRNA / PT-PCR / Polymorphism / Alcohol,ethyl / Alchol,ethyl / Polymosphism |
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| Research Abstract |
It is well known that the Oriental flushers, homozygotically possessingALDH2*2, show dysphoric symptoms after intake of even small doses of ethanol. Heterozygotic subjects (ALDH2*2/*1) can, however, drink a small amount and then after a few hours drink a little more alcohol, with the dysphoric symptoms diminish somewhat to the second challenge. The main purpose of this study was to determine whether the levels of mRNA for ALDH2 are different before and after ethanol intake.
A method for determining mRNA levels for ALDH2 in peripheral blood was developed using an RT-PCR technique. DNA-free total RNA was extracted from peripheral lymphocytes from healthy Japanese volunteers. Purified RNA was reverse transcribed into cDNA.The cDNA was amplified with PCR using the primers, designed through exon 11 and 12-13. PCR DNA products were
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