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Immunohistochemical study on neuronal changes in brainstem nucleus of sudden infant death syndrome and various caouses of death

Research Project

Project/Area Number 09670442
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Legal medicine
Research InstitutionThe University of Tokushima

Principal Investigator

KUBO Shin-ichi  The Univ.of Tokushima, School of Med., Professor, 医学部, 教授 (10205122)

Co-Investigator(Kenkyū-buntansha) GOTOHDA Takako  The Univ.of Tokushima, School of Med., Assistant, 医学部, 助手 (50304506)
ORIHARA Yoshiyuki  Nagasaki Univ., School of Med., Assistant, 医学部, 助手 (70264215)
TOKUNAGA Itsuo  The Univ.of Tokushima, School of Med., Lecturer, 医学部, 講師 (30116842)
Project Period (FY) 1997 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 1998: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1997: ¥2,700,000 (Direct Cost: ¥2,700,000)
Keywordsneuropathology / immunohistochemistry / brainstem / cause of death / suuden infant death syndrome / asphyxia / HSP70 / c-Fos / 乳幼児急死症候群 / 窒素死
Research Abstract

Scvcral nuclei in brain stem are well known to play an important role in supporting human life. However, the connection between neural changes of brain stem and the cause of death is not yet fully understood. To investigate the correlation of brain stem damage with the cause of death in forensic autopsy cases, neural changes of several nuclei were examined using immunohistochemical technique. Based in the cause of death, the forensic autopsy cases were divides into 6 groups as follows. Group A : hanging, ligature strangulation and manual strangulation, Group B : smothering arid choking, Group C : drowning, Group D : sudden infant death syndrome(SIDS), Group E : respiratory failure, Group F : heat stroke and sun stroke. The brain-stem were stained with the antibodies against microtuble-associated protein 2 (MAP2), muscalinic acetycholine receptor (mACh-R), c-fos gene product (c-Fos) and 72 kD heat-shock protein (HSP70), and the immunostaining of the-se markers in the arcuate nucleus (ARC), the hypoglossal nucleus (HN) and inferior olive (I0) was examined. In ease of asphyxia, neurons in HN were positively stained with both HSP70 and c-Fos antibodies, although morphological changes of these neurons was not evident. This may indicate that the occlusion of upper airway results in the damage to neurons in HN without their morphological changes. Positive staining of HSP70 and c-Fos in IO was more frequently observed in Group C than other groups. Since IO is involved in maintaining body balance which is often disturbed by drowning, it seems possible that neuronal damage in IO observed in drowning may be related to the disturbance of body balance. Consistent with the finding reported previously, the number of neurons with positive staining of mAChR in Group D was less than that in other groups. These observations indicate that immnuohistochemical study on the damage to neurons in brain stein nuclei can provide useful information for determining the cause of death.

Report

(3 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • Research Products

    (6 results)

All Other

All Publications (6 results)

  • [Publications] 久保真一外: "法医剖検例における脳幹部神経核の免疫組織化学的検討 I.窒息および呼吸障害について" 日本法医学雑誌. 56(6)印刷中. (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 久保真一外: "法医剖検例における脳幹部神経核の免疫組織化学的検討 II.乳幼児突然死症候群について" 日本法医学雑誌. 56(6)印刷中. (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] S.Kubo, Y.Orihara, T.Gotohda, I.Tokunaga, R.Tsuda, K.Ikematsu, O.Kitamura, A.Yamamoto and I.Nakasono: "Immunohistochemical study on neuronal changes in brainstem nuclcus of forensic autopsy cases I.Various cases of asphyxia and respiratory disorder" Japanese J.Legal Medicine. 56 (6) (in press). (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] S.Kubo, Y.Orihara, T.Gotohda, R.Tsuda, K.Ikematsu, O.Kitamura, A.Yamamotoand I.Nakasono: "Immunohistochemical study on neuronal changes in brainstem nucleus of forensic autopsy cases H.Sudden infant death syndromc." Japanese J.Legal Medicine. 56 (6) (in press). (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 久保新一ら: "法医剖検例における脳幹部神経核の免疫組織科学的検討I.窒息及び呼吸障害について" 日本法医学雑誌. 56(6)(印刷中). (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 久保新一ら: "法医剖検例における脳幹部神経核の免疫組織科学的検討II.乳幼児突然死症候群について" 日本法医学雑誌. 56(6)(印刷中). (1998)

    • Related Report
      1998 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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