| Project/Area Number |
09670445
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Legal medicine
|
|---|
| Research Institution | Kagoshima University |
|---|
Principal Investigator |
TSUGANEZAWA Osao Faculty of Medicine Kagoshima University, Professor, 医学部, 教授 (80064517)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
AGO Mihoko Faculty of Medicine Kagoshima University, Research Associate, 医学部, 助手 (70041340)
AGO Kazutoshi Faculty of Medicine Kagoshima University, Assistant Professor, 医学部, 講師 (20102056)
OGATA Mamoru Faculty of Medicine Kagoshima University, Associate Professor, 医学部, 助教授 (10152373)
|
|---|
| Project Period (FY) |
1997 – 1998
|
| Project Status |
Completed (Fiscal Year 1998)
|
| Budget Amount *help |
¥2,400,000 (Direct Cost: ¥2,400,000)
Fiscal Year 1998: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1997: ¥1,700,000 (Direct Cost: ¥1,700,000)
|
|---|
| Keywords | medicolegal autopsy / compression of the neck / mechanical asphyxia / compression marks on the skin / Histological staining / collagen fibers / elastic fibers |
|---|
| Research Abstract |
In this study, an attempt was made to devise a more reliable staining technique for showing evidence of compression marks on the skin. For this purpose, 40 dyestuffs were examined on their staining capacity for distinguishing compressed skin from normal. Neck skin specimens were taken from autopsy cases with mechanical asphyxia, ligature and manual strangulation. Specimens were fixed in 20% neutral buffered formalin in a condition of being flatwise stretched, embedded in paraffin and cut parallel to the surface of the skin. Negative control specimens were taken from area without any lesions. As a result of tests, it was found the following two combinations of dyes gave alike good results :
(1) Chromotrope 2R, Light green SF and Phosphotungstic acid (Gomori technique), (2) Orange G, Light green SF and Phosphotungstic acid (referred to as OL technique). These techniques were combined with elastic fiber staining by orcein methods. Results of the combination of orcein and Gomori technique (1) were as follows : In the compressed area, dermal collagen stained red and elastic fibers were in disarray and/or severed ; On the other hand, dermal collagen stained green and elastic fibers were in array without severance in the normal area. Results of the combination of orcein and OL technique (2) were as follows : In the compressed area dermal collagen stained orange-yellow and elastic fiber were in disarray and/or severed ; In the normal area, dermal collagen stained yellowish green and elastic fibers were in array without severance. When the compression was inflicted post mortem, compressed dermis gave the same reaction as dermis compressed ante mortem. The post mortem compression was able to cause severing of elastic fibers, but not disarray of them. Therefore, it was concluded that the technique proposed here is valuable when seeking evidence of compression marks.
|
