• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Immunohistochemical and Ultrastructual Study of Brain Injury Produced by Repeated Impacts.

Research Project

Project/Area Number 09670450
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Legal medicine
Research InstitutionIwate Medical University

Principal Investigator

AOKI Yasuhiro  School of Medicine, Iwate Medical University, Professor, 医学部, 教授 (90202481)

Co-Investigator(Kenkyū-buntansha) NAKAYAMA Yumi  School of Medicine, Iwate Medical University, Research Associate, 医学部, 助手 (10295967)
Project Period (FY) 1997 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 1999: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1998: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1997: ¥2,400,000 (Direct Cost: ¥2,400,000)
Keywordsbrain injury / axonal injury / immunohistochemistry / animal experiment / cell culture / ultramicrostructure / 免疫組織化学 / fluid percussion モデル / 三叉神経節 / fluid percussionモデル
Research Abstract

To investigate mechanical effect of repeated minor impact to the pathobiology of the brain, 2 experimental head injury models were designed. In vivo, adult rats were injured utilizing weight-drop device consisting of brass weight(450g) falling through a acrylic guide tube(1m). After 3 insults at an interval of 24 hours, the brain were perfused with 4% Paraformaldehyde in phosphate buffer solution and removed. Histopathological and immunohistochemical studies disclosed neuronal injury in the cerebral cortex, and brain edema in the corpus callosum. Despite the lack of diffuse axonal changes, this model would be suitable for studying neuronal, changes associated with minor head trauma. In vitro model of axonal injury using cultured cells was designed to introduce traumatic alterations on neuronal processes and to identify mechanisms responsible for the formation of focal swellings by observation with phase-contrast and transmission electron microscopes. The culture dishes were oscillated … More one-dimensionally and horizontally 1, 2 or 3 times; repeating at intervals of an hour, 5 seconds a time; on a heat controlled shaker. The amplitude and frequency of the oscillation was 40 mm and 1 Hz, respectively. The injured processes showed two forms: the terminal increase in diameter of the processes and beading depending on injured portions. The microscopic finding suggests that the stress would destroy a cytoskeletal network, and then cause the spherical deformation of the processes. Ultramicroscopically, the beads contained compactly and sparsely misaligned cytoskeletons and prominently gathered densecore vesicles. The cytoskeletal reconstruction within the regenerated growth cones was also noted in the terminal swellings and beads. The destruction of these linkers would be responsible to initiate the cytoskeletal destruction. The initiating point of the cytoskeletal alterations, however, must be confirmed using three dimensional imaging of samples without soluble proteins and immunohistochemical study. Less

Report

(4 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • 1997 Annual Research Report
  • Research Products

    (8 results)

All Other

All Publications (8 results)

  • [Publications] Nakayama Y, Niitsu H, Aoki Y.: "The ultrastractural alterations of processes of the trigeminal ganglion cell in culture by repeated acceleration and decceleration"6th Indo Pacific Congress on Legal Medicine and Forensic Sciences. 991-994 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Nakayama Y, Aoki Y.: "Mechanism responsible for formation of focal swelling on injured neuronal processes: Using a novel in vitro model of axonal injury"Forensic Science International(Suppl.). (in press). (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Nakayama Y, Aoki Y.: "tudies on the mechanisms responsible for formation of focal swelling on neuronal processes using a novel in-vitro model of axonal injury."Journal of Neurotrauma. (in press). (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Nakayama Y, Niitsu H, Aoki Y: "The ultrastructural alterations of processes of the trigeminal ganglion cell in culture by repeated acceleration and decceleration."6th Indo Pacific Congress on Legal Medicine and Forensic Sciences. 991-994 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Nakayama Y, Aoki Y: "Mechanism responsible for the formation of focal swelling on injured neuronal processes: Using a novel in vitro model of axonal injury."Forensic Science International (Suppl.). (in press). (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Nakayama Y, Niitsu H, Aoki Y: "Studies on the mechanisms responsible for formation of focal swelling on neuronal processes using a novel in-vitro model of axonal injury."J Neurotrauma. (in press). (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Nakayama Y,Niitsu H,Aoki Y.: "The ultrastractural alterations of processes of the trigeminal ganglion cell in culture by repeated acceleration and decceleration"6th Indo Pacific Congress on Legal Medicine and Forensic Sciences. 991-994 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Nakayama Y,Aoki Y.: "Mechanism responsible for the formation of focal swelling on injured neuronal processes:Using a novel in vitro model of axonal injury"Forensic Science International(Suppl.). (in press). (2000)

    • Related Report
      1999 Annual Research Report

URL: 

Published: 1997-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi