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Intercellular action between human lung mast cells and eosinophils

Research Project

Project/Area Number 09670465
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 内科学一般
Research InstitutionGunma University

Principal Investigator

KANAZAWA Tsugio  Gunma University, School of Health Sciences, 医学部・保健学科, 教授 (70008316)

Project Period (FY) 1997 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥2,600,000 (Direct Cost: ¥2,600,000)
Fiscal Year 1998: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1997: ¥2,100,000 (Direct Cost: ¥2,100,000)
KeywordsHuman lung mast cell / eosinophil / IL-3 / IL-5 / TNF-alpha(tumor necrosis factor alpha) / GM-CSF(granulocyte-macrophage colony stimulating factor) / TNF-α / GM-CSF / eosinophil cationic protein
Research Abstract

Recent works suggest that network of intercellular reactions among inflammatory cells including lymphocytes, mast cells and eosinophils contribute to production of chronic allergic inflammation. Aim of this study is to search intercellular action between mast cell and eosinophils in human lung. By using RT-PCA, in site hybridization, enzyme immunoassay and immunocytometry, we studied the production of GM-CSF(granulocyte-macrophage colony stimulating factor), IL-3, IL-5 and TNF- alpha (tumor necrosis factor alpha) in human lung mast cells and the localization of mRNA of these cytokines, and studied their effect to eosinophils.
Purified human lung mast cells challenged with anti-IgE produced inflammatory cytokines such as GM-CSF,IL-3, IL-5 and TNF- alpha protein 4-8 hours after the activation. The mast cells supernatant induced ECP(eosinophil cationic protein) release from eosinophils and the release was significantly inhibited by blocking monoclonal antibodies against GM-CSF, IL-5, and TNF- alpha.
Corticosteroid (beta methasone) suppressed dose-dependently suppressed the release of GM-CSF from human lung mast cells. TNF- alpha was produced by stimulation of substance P.It suggests that the cytokine is also released by non-IgE stimulation.
These findings indicate that human mast cells are an important cellular source of various cytokines and may contribute to chronic eosinophil-mediated inflammation.

Report

(3 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • Research Products

    (9 results)

All Other

All Publications (9 results)

  • [Publications] Okayama Y et al: "Human lung mast cells are enriched in the Capacity to produce gronulocyte-macropkage Colony stimulating factor in response to lgE stimulation" Eur J Immunol. 28. 708-715 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Okayama Y et al: "Human skin mast cells produce TNFd by substance P" Int Arch Allergy Immunol. 11. 48-51 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Nakazawa T et al: "Pathological features of the lung obtained from fatality of occupational astiwa sensitized wita wheat flour" Advances in the prevention of occupational sespiratory diseases. 9. 790-792 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Nakazawa T: "Occupatioral hypersensitivity pneumutis lue to mushroom spores" Advances in the prevention of occupational sespiratory diseases. 9. 1206-1208 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Okayama Y,Kobayashi H,Ashman LK,Dobashi K Nakazawa T,Holgate ST,Church MK,Mori M: "Human lung mast cells are enriched in the capacity to produce granulocyte-macrophage colony-stimulating factor in response to IgE dependent stimulation" Eur J Immunol. 28. 708-715 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Okayama Y,Ono Y,Nakazawa T,Church MK Mori M: "Human skin mast cells produce TNF-alpha by substance P" Int Arch Allergy Immunol. 117. 48-51 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Okayama Y: "Human lung most cells are enriched in the capacity to produce granulocyte-Nacrophage colony-Stimulation Factor in vesponse to IgE-dependent Stimulation" Eur J Immunol. 28. 708-715 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Okayama Y: "Human Skin mast cells produce TNF-α by substance P" Int Arch Allergy Immunol. 117. 48-51 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Y.Okayama, H.Kobayashi L.Asnman, K.Dobashi, T.Nakazawa et al: "Human lung mast cells are enriched in the capacity to produce granulocyte-macrophage colony-stimulating factor in response to IgE-dependent" Eur,J.Immunol. 28. 708-715 (1998)

    • Related Report
      1997 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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