| Project/Area Number |
09670474
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
内科学一般
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| Research Institution | The University of Tokyo |
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Principal Investigator |
HONDA Zenichiro Univ.of Tokyo, faculty of Medicine, Associate Professor, 医学部・附属病院, 講師 (70238814)
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| Co-Investigator(Kenkyū-buntansha) |
SHIMIZU Takao Univ.of Tokyo, Assistant Professor, 医学部・生化学教室, 教授 (80127092)
KUME Kazuhiko Univ.of Tokyo, Assistant Professor, 医学部・生化学教室, 助手 (30251218)
HIROSE Naoto Univ.of Tokyo, faculty of Medicine, Assistant Professor, 医学部・附属病院, 助手 (60261971)
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| Project Period (FY) |
1997 – 1998
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| Project Status |
Completed (Fiscal Year 1998)
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| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 1998: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 1997: ¥2,100,000 (Direct Cost: ¥2,100,000)
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| Keywords | Fc receptor / Src fanuly protein tyrosine kinase / C-Sre / Lyn / Fyn / C-terminel Src Kinase / Plapocytosin / Chemotaxis / Fe受容体 / Lun / c粒tinsreキナーゼ / 貪食 / Src型キナーゼ / C末端srcキナーゼ / CsK / 顆粒分泌 / 接着 |
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| Research Abstract |
We have analysed the mechanisms, from the view of the relevance of Src family kinases, of base inflemmetory processes of 1)Fc receptor-mediated ^-Ca^<++> inflax and degranulation 2)Fc receptor-mediated phagocytosis, and of 3)Integrin-mediated cell adhesion and locomotion. By using a model mact cell live, and a model macrophage line variously expressing Src family kinases, e-terminal Src kinases, and their mertated forms in various combinations, we have revealsd.
1)Fc-reeptor mediated Ca^<++> inflax and degranulation require Src family kinases.
2)Lyn, and Fyn can transmit the signal, but C-Be connot.
3)Both SH_4, and SH_2 domains of Lyn are required, but SH_3 domain is not required for the functions.
4)Fc-receptor mediated particle ingestion requires Src family kinases.
5)HCK and Lyn can catalyse the reaction, C-Snc cannot.
6)Integrin-mediated podosome formation of medicated both by Lynand C-Src.
7)Only Lyn catalyze Integrin-medicated chemotaxis.
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