| Project/Area Number |
09670496
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
内科学一般
|
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| Research Institution | St.Marianna University School of Medicine |
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Principal Investigator |
KATO Tomohiro St.Marianna University School of Medicine Institute of Medical Science Associate professor, 難病治療研究センター, 講師 (80233807)
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| Project Period (FY) |
1997 – 1998
|
| Project Status |
Completed (Fiscal Year 1998)
|
| Budget Amount *help |
¥2,900,000 (Direct Cost: ¥2,900,000)
Fiscal Year 1998: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 1997: ¥1,600,000 (Direct Cost: ¥1,600,000)
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| Keywords | systemic lupus erythematosus / Tcell / antigen-specific reaction / auto antigen |
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| Research Abstract |
Many lines of studies suggested that T cells play pivotal roles in the pathogenesis of systemic lupus erythematosus (SLE). We reported oligoclonal T cell proliferation in peripheral blood of patients with the disease previously. To identify the antigen peptides for the accumulated T cell clonotypes, we developed a T cell receptor cloning method from a single cell for the reconstruction of TCR molecules. Further, we successfully expressed HLA class I molecules with beta-2-microglobulin on the filamentous phages as a single chain for constructing an antigen peptide library. Screening of the library with the reconstructed TCR would determine candidate antigens for the proliferating T cells in SLE.
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