• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Clarification of microbial agents in Crohn's disease by representational difference analysis

Research Project

Project/Area Number 09670506
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Gastroenterology
Research InstitutionAKITA UNIVERSITY

Principal Investigator

CHIBA Mitsuro  School of Medicine, Akita University, Associate Professor, 医学部, 助教授 (90150250)

Project Period (FY) 1997 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 1999: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1998: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1997: ¥2,300,000 (Direct Cost: ¥2,300,000)
KeywordsCrohn's disease / ulcerative colitis / lymph follicle / etiology / bacteria / ribosomal RNA / microbiology / PCR / クローン病Cnohn's disease / 16S rRNA / Staphylococcus aureus / 炎症性腸疾患 IBD / クローン病 Crohn's disease / クローン病 Crohn's diseace / Mycobacterium paratuberculosis / Listeria monocytogenes
Research Abstract

It has been suggested that microbial agents are involved in the onset of Crohn's disease. None of the candidates, however, have been unequivocally demonstrated to be causative agents. Based on clinical and immunological studies, we proposed the need of microbiology of the lymph follicle to identify causative agents in Crohn's disease. By polymerase chain reaction (PCR) using universal primers designed from conserved regions of bacterial 16S ribosomal RNA (rRNA) and subsequent DNA sequence analysis followed by a database homology search, we first confirmed the presence of bacterial 16S rRNA gene segments in human intestinal lymph follicles in 8 cases. Bacterial 16S rRNA gene segments were detected in the lymph follicle in 2 of 14 (14%) non-inflammatory bowel diseases (IBD) cases, 4 of 14 (28%) Crohn's disease cases, and in 2 of 5 (40%) ulcerative colitis cases. Nineteen 16S rRNA gene segments were recognized. Five segments showed 100% identity to known bacterial 16S rRNAs, namely staphylococcus species, Streptococcus sanguis, and Paracoccus marcusii.
However, the other 14 segments showed below 100% identity, indicating either the presence of unknown bacteria or of bacteria without known DNA data. No single identified or unidentified bacterium was characteristic for inflammatory bowel diseases. In order to clarify the etiologic significance of Mycobacterium paratuberculosis, and Listeria monocytogenes, Staphylococcus aureus (staphylococcal superantigens) in Crohn's disease, we investigated whether they could be detected in intestinal tissues, including Peyer's patches and colonic lymph follicles. Since none of tissues was positive for these bacteria, it was concluded that they were not involved in the etiology of Crohn's disease. During the process of studies, cases of intestinal infections of Listeria monocytogenes or Staphylococcus aureus was first confirmed in resected specimens of ulcerative colitis.

Report

(4 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • 1997 Annual Research Report
  • Research Products

    (28 results)

All Other

All Publications (28 results)

  • [Publications] Chiba M: "Listeria monocytogenes in Crohn's disease"Scandinavian J Gastoenterology. 33. 430-434 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Chiba M: "No detection of Mycobacterium paratuberculosis in intestinal tissue including Peyer's patches and lymph follicles in Crohn's disease"J Gastoenterology. 33(4). 482-487 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Chiba M: "Microbiology of the intestinal lymph follicle : A clue to elucidate causative microbial agent(s) Crohn's disease"Medical Hypotheses. 51. 421-427 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Chiba M: "Listeria monocytogens in the colon in a case of fulminant ulcerative colitis"Scandinavian J Gastoenterology. 33. 778-782 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Chiba M: "Presence of bacterial 16S ribosomal RNA gene segments in intestinal lymph follicles"Scandinavian J Gastoenterology. (in press). (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Chiba M: "Staphylococcus aureus in inflammatory bowe1 diseases"(submitted).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 千葉満郎: "炎症性腸疾患"消火器病セミナー・77 へるす出版. 320 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 千葉満郎: "炎症性腸疾患"医学書院. 301 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Chiba M, Komatsu M, Iizuka M, Masamune O, Hoshina S, Kono M.: "Microbiology of the intestinal lymph follicle. A clue to elucidate causative agent(s) in crohn's disease."Medical Hypotheses. 51. 421-427 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Chiba M, Fukushima T, Inoue S, Horie Y, Iizuka M, Masamune O.: "Listeria monocytogenes in Crohn's disease."Scand J Gastroenterol. 33. 430-434 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Chiba M, Fukushima T, Horie Y, Iizuka M, Masamune O.: "No Mycobacterium paratuberculosis detected in intestinal tissue, including Peyer's patches and lymph follicles, of Crohn's disease."J Gastroenterol. 33. 482-487 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Chiba M, Fukushima T, Koganei K, Nakamura N, Masamune O.: "Listeria monocytogenes in the colon in a case of fulminant ulcerative colitis."Scand J Gastroenterol. 33. 778-782 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Chiba M, Kono M, Hoshina S, Komatsu M, Kitagawa Y, Iizuka M, Watanabe S.: "Presence of bacterial 16S ribosomal RNA gene segments in human intestinal lymph follicles. (accepted for publication)"Scand J Gastroenterol. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Chiba M, Hoshina S, Kono M, Tobita M, Fukushima T, Iizuka M, Watanabe S.: "Staphylococcus aureus in inflammatory bowel diseases."(submitted).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Chiba M, Iizuka M.: "Intestinal microflora and viruses. Inflammatory bowel disease"

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Chiba M.: "Current topics."

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Bamba T. ed.: "Seminar in Gastroenterology No.77"Health Pub. Tokyo. 45-56 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Muto T, et al. Ed.: "Inflammatory bowel disease"Igaku-Shyoin Co, Tokyo. 83-85 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Chiba M,et al.: "Presence of bacterial 165 ribosomal RNA gene segments in human intestinal lymph follicles"Scandi J Gastroenterol. (in press). (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] 千葉満郎,飯塚政弘: "炎症性腸疾患 腸内細菌・ウイルス"消化器病セミナー・77 へるす出版 馬場忠雄(編). 320 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 千葉満郎: "炎症性腸疾患"医学書院(武藤徹一郎,八尾恒良,名川弘一,桜井敏弘(編). 301 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Chiba M: "Listeria monocytogenes in Crohn′s disease" Scandinavian J Gastroenterology. 33. 430-434 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Chiba M: "No detection of Mycobacterium paratuberculosis in intestinal tissue including peyer's patches and lymph follicles of Crohn′s disease" J Gastroenterology.33(4). 482-487 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Chiba M: "Microbiology of the intestinal lymph follicle-A clue to elucidate causative microbial gent(s) in Crohn′s disease" Medical Hypotheses. 51. 421-427 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Chiba M: "Listeria monocytogenes in the colon in a case of fulminant ulceratiue colitis" Scandinavian J Gastroenterology. 33. 778-782 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Chiba M: "Listeria monocytogenes in Crohn's diseace" Scandinavian J Gastroenterology. 33(印刷中). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Chiba M: "No detection of Mycobacterium paratubesculosis in intestinal tessue including Peyer's patches and lymph follicles of crohn's disease" J Gastroenterology. 33(4)(印刷中). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Chiba M: "Microbiology of the intestinal lymph follicle-A clue to elucidate causative microbial gents in crohn's disease" Medical Hypotheses. 47(印刷中). (1998)

    • Related Report
      1997 Annual Research Report

URL: 

Published: 1997-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi