| Project/Area Number |
09670542
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Gastroenterology
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| Research Institution | Shiga University of Medical Science |
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Principal Investigator |
TSUJIKAWA Tomoyuki (1999) Shiga University of Medical Science, Department of Internal Medicine, Instructor, 医学部, 助手 (80273407)
藤山 佳秀 (1997-1998) 滋賀医科大学, 医学部, 助教授 (70111896)
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| Co-Investigator(Kenkyū-buntansha) |
辻川 知之 滋賀医科大学, 医学部, 助手 (80273407)
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| Project Period (FY) |
1997 – 1999
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| Project Status |
Completed (Fiscal Year 1999)
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| Budget Amount *help |
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 1999: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1998: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1997: ¥1,800,000 (Direct Cost: ¥1,800,000)
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| Keywords | SEB / DSS colitis / TCR α KO mice / Vβ8T-cell / oral torelance / TCRα鎖欠損マウス / コラーゲン誘導関節炎 / 自己免疫疾患 / 経口寛客 / スーパー抗原 / 炎症性腸疾患(IBD) |
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| Research Abstract |
We have been elucidated that oral SEB administration prevented the onset of DSS induced colitis, and influence of clonal deletion designated by Vβ8T -cells. We evaluated the effect of oral SEB administration on TCR α KO mice. (Experiment 1) Simultaneous administration of SEB ameliorated DSS induced colitis on TCR α KO mice, following prolonged survival time. Although serum Ig M of mice administered DSS increased more than that of control, the cell fractions of CD4ィイD1+ィエD1TCRVβィイD1dimィエD1T-cells and CD4ィイD1+ィエD1TCRVβィイD1dimィエD1T-cells did not change with or without SEB oral administration. (Experiment 2) Although TCR α KO mice have been given SEB for 9 weeks, the incidence of colitis did not decrease and the cell fractions of CD4ィイD1+ィエD1TCRVβィイD1dimィエD1T-cells also did not change. It was suggested that CD4ィイD1+ィエD1TCRVβィイD1dimィエD1T-cell may influence the onset of the colitis on TCR α KO mice. We elucidated that preventive effect of SEB on DSS induced colitis of TCR α KO mice. Therefore, it is suggested that TCRαィイD1-ィエD1βィイD1+ィエD1 cells which express the Vβ region corresponding SEB may play an important role on the onset of DSS induced colitis on TCR α KO mice. However, it was difficult to induce oral tolerance by SEB under condition of continuing local inflammation such as colitis of TCR α KO mice.
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