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Reg-mediatedproliferative effect of gastrin on gastric epithelial cell and cancer cell

Research Project

Project/Area Number 09670548
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Gastroenterology
Research InstitutionShimane Medical University

Principal Investigator

KINOSHITA Yoshikazu  Shimane Medical University, Professor, 医学部, 教授 (30243306)

Co-Investigator(Kenkyū-buntansha) CHIBA Tutomu  Kyoto University Graduate School of Medicine Dept of Gastroentel and Hepatol, Pr, 大学院・医学研究科, 教授 (30188487)
Project Period (FY) 1997 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥2,800,000 (Direct Cost: ¥2,800,000)
Fiscal Year 1998: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1997: ¥1,600,000 (Direct Cost: ¥1,600,000)
Keywordsgstrin / gastric mucosa / growth factor / ECL cell
Research Abstract

The research was performed to test the hypothesis that gastrin Indirectly stimulates the proliferation of gastric epithelial cells through the increased production of Reg protein by entero chromaff in likecells (ECL cells) . For this purpose, in vivo and in vitro studies were done on rats as an experimental model. The chronic and acute elevation of plasma gastrin concentration in rats remarkably dugmented the Reg mRNA and Reg protein contents in gastric ECL cell. This effect of gastrin was almost completely blocked by the administration of a gastrin receptor antagonist. When the gene expression of Reg was stimulated, the profile ration of gastric epithelial cell in gland neck proliferation zone was also stimulated. Recombinant reg protein, when added in the cell culture of rat gastric proliferating epithelial cells, dose-dependently stimulated the proliferation of the cells. These data clearly showed that gastrin stimulates the gastric epithelial proliferation through the increased production of Reg protein by ECL cells.

Report

(3 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • Research Products

    (85 results)

All Other

All Publications (85 results)

  • [Publications] Kinoshita Y.et al: "Production and activation of hepatocyte growth factor" Digestion. 58. 225-231 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Ding M, et al: "Distribution of prostaglandin E receptors in the rat" Prostaglandins. 53. 199-216 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kawanami C, et al: "Regenerating gene expression in normal gastric mucosa" J.Gastroenterol. 32. 12-18 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Morimoto K, et al: "Cellular localization of mRNAs for the prostaglandin E" Am.J.Physiol. 272. G681-G687 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Wang HY, et al: "Developmental gene expression of gastrin receptor" Regul Pep. 70. 183-189 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Maekawa T, et al: "Helicobacter pylori induced proinflammatory cytokines" J Lob Clin Med. 130. 442-449 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kinoshita Y, et al: "Helicobacter pylori-independent chronological change of gastric acid" Gut. 41. 452-458 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Waki S, et al: "Sever muscle damage induced by high carbohydrate" J.Grastroenterol. 33. 121-124 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Waki S, et al: "Intragastric distribution of NSAID-related ulcers" J.Clin.Gastroenterology. 25. 592-594 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kishi K, et al: "Pepsinogen C gene expression in gastritis" Biochem Biophys Res Commun. 238. 17-20 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Waki S, et al: "Effect of aging on gastrin receptor gene expression in rat stomach" Peptides. 19. 225-229 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Yoshida M, et al: "Trichuris Trichiura infection" Am J Gastroenterol. 92. 719-720 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Hassan S, et al: "Expression of the proto-oncogene c-kit and its ligand" Dig.Dis.Sci. 43. 8-14 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kinoshita Y, et al: "Increased hepatocyte growth factor content in rat stomach during omeprazole" Digestion. 59. 102-109 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Okada A, et al: "Rat gastric mucosal cells express ICAM-1 and proinflammatory cytokines" J Lab Clin Med. 131. 538-547 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Hassan S, et al: "Geranylgeranylacetone(GGA), an anti ulcer drug, stimulates hexosamine" J.Gastroenterol Hepatol. 13. 809-815 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Maekawa T, et al: "Relation between severity and symptoms of reflux esophagitis in elderly" J.Gastroenterol Hepatol. 13. 927-930 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kinoshita Y, et al: "Gastric MAL Toma with M proteins in serum and gastric juice." Gastroenterology. 114. 1353-1354 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Aoyama N, et al: "Peptic ulcers after the Hanshin-Awaji earthquake : Increased incidence of" Am J Gastroenterol. 93. 311-316 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Maekawa T, et al: "Presence of atrial natriuretic peptide in rat thyroid wedullary carcinoma cell" Acta Oncologica. 37. 500-502 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Matsushima Y, et al: "Augmentation of water-immersion-stress induced gastric mucosal lesions" Digestion. 60. 34-40 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Okuda S, et al: "Fetal liver cirrhosis and esophageal variceal hemorrhage in a patients with" Internal Medicine. 37. 1055-1057 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kinoshita Y, et al: "Comparison of the signal transduction pathways activated by gastrin in" Gastroenterology. 115. 93-100 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Maekawa T, et al: "Reciprocal changes in progression of gastric MALT lymphoma and" Am J Med. 105. 539-541 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Fukui H, et al: "Reg protein may mediate gastric mucosal proliferation induced by" Gastroenterology. 115. 1483-1493 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Tanaka S, et al: "Laparoscopic fenestration in combination with ethanol sclerotherapy" Surg Laparosc Endosc. 8. 453-456 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kazumori H, et al: "Primary sclerosing pancreatitis and cholangitis." International Journal of Pancreatology. 24. 123-127 (1198)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kinoshita Y,et al.: "Production and activation of hepatocyte growth factor during the healing of rat gastric ulcers." Digestion. 58. 225-231 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Ding M.et al.: "Distribution of prostaglandin E receptors in the rat gastrointestinal tract." Prostaglandins. 53. 199-216 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kawanami C,et al.: "Regeneratinggene expression in norma gastric mucosa and indomethacin-inducedmucosal" Gastroenterol. 32. 12-18 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Morimoto K,et al.: "Cellular localization of mRNAs for the prostaglandin E receptor subtypes in the mouse" Am J Physiol. 272. G681-G687 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Wang HY,et al.: "Developmentalgene expression of gastrin receptor in rat stomach." Regul Pep. 70. 183-189 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Maekawa T,et al.: "Helicobacter pylori induced proinflammatory cytokines and major histocompatibility complex class II antigen in mouse gastric epithelial cells." J Lab Clin Med. 130. 442-449 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kinoshita Y,et al.: "Helicobacterpylori-independent chronological changeof gastric acid secretion in Japanese." Gut. 41. 452-458 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Waki S,et al.: "Intragastric distribution to NSAID-related ulcers-Study on subjects without collagen diseases-." J Clin Gastroenterology. 25. 592-594 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kishi K,et al.: "Pepsinogen C gene expression in gastritis." Biochem Biophys Res Common. 238. 17-20 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Yoshida M,et al.: "Trichuris Trichiura infection, abdominal pain, and colonoscopy." Am J Gastroenterol. 92. 719-720 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Waki S,et al.: "Sever muscle damageinduced by high carbohydrateintake from elemental diet in a patient with Crohn's disease." J.Gastroenterol. 33. 121-124 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Hassan S,et al.: "Expression of the proto-oncogenec-kit and its ligand stem cell factor (SCF) in gastric carcinoma cell lines." Dig.Dis.Sci.43. 8-14 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kinoshita Y,et al.: "Increasedhepatocyte growth factor content in rat stomach during omeprazole treatment." Digestion. 59. 102-109 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Waki S,et al.: "Effect of aging on gastrin receptor gene expression in rat stomach." Peptides. 19. 225-229 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Okada A,et al.: "Rat gastric mucosal cells express ICAM-1 amd proinflammatory cytokiness during indomethacin inducedmucosalinjury." J Lab Clin Med. 131. 538-547 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Hassan S,et al.: "Geranylgeranylacetone (GGA), an antiulcer drug, stimulates hexosamine production in a rat gastri mucosal cell line through binding to a specific cytosolic protein." J.Gastoroenterol Hepatol. 13. 809-815 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Maekawa T,et al.: "Relation between severity and symptoms of reflux esophaagitis in eldely patients in Japan." J.Gastroenterol Hepatol. 13. 927-930 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kinoshita Y,et al.: "Gastric MALToma with M proteins in serum and gastric juice." Gastroenterology. 114. 1353-1354 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Aoyama N,et al.: "Peptic ulcers after the Hanshin-Awaji earthguake : Increased incidence of bleeding gastric" Am J Gastroenterol. 93. 311-316 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Maekawa T,et al.: "Presence of atrial natriuretic peptide in rat thyroid wedullary carcinoma cell line rMTC6-23." Acta Oncologica. 37. 500-502 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Okuda S,et al.: "Fetal liver cirrhosis and esophagealvariceal hemorrhagein a patients with type (ki)a glycogen strage disease." Internal Medicine. 37. 1055-1057 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kinoshita Y,et al.: "Comparison of the signal transduction palthways activated by gastrin in Enterochromaffin-like and parietal cells." Gastroenterology. 115. 93-100 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Maekawa T,et al.: "Reciprocal changes in progression of gastric MALT lymphoma and pancreatic somatostationoma." Am J Med. 105. 539-541 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Fukui H,et al.: "Reg protein may mediate gastric mucosal proliferation induced by hypergastrinemiain rats." Gastroenterology. 115. 1483-1493 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Tanaka S.et al.: "laparoscopic fenestration in combination with ethanol slerotherapy prevents a recurrenceof symptomatic giant liver cyst." Surg Laparosc Endosc. 8. 453-456 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kazumori H,et al.: "Primary sclerosing pancreatitis and cholangitis." International Journal of Pancreatology. 24. 123-127 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Matsushima Y,et al.: "Augmentation of water-immersion-stress inducedgastric mucosal lesions in BALB/c mice infected with Helicobacterfelis." Digestion. 60. 34-40 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kinoshita Y,et al: "Increased hepatocyte growth factor content in rat stomach during omeprazole" Digestion. 59. 102-109 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Okada A,et al: "Rat gastric mucosal cells express ICAM-1and proinflammatory cytokines" J Lab Clin Med. 131. 538-547 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Hassan S,et al: "Geranylgeranylacetone (GGA), an anti ulcer drug,stimulates hexosamine" J.Gastroenterol Hepatol. 13. 809-815 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Maekawa T,et al: "Relation between severity and symptoms of reflux esophagitis in elderly" J.Gastroenterol Hepatol. 13. 927-930 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Kinoshita Y,et al: "Gastric MALToma with M proteins in serum and gastric juice." Gastroenterology. 114. 1353-1354 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Aoyama N,et al: "Peptic ulcers after the Hanshin-Awaji earthquake : Increased incidence of" Am J Gastroenterol. 93. 311-316 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Maekawa T,et al: "Presence of atrial natriuretic peptide in rat thyroid wedullary carcinoma cell" Acta Oncologica. 37. 500-502 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Matsushima Y,et al: "Augmentation of water-immersion-stress induced gastric mucosal lesions" Digestion. 60. 34-40 (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Okuda S,et al: "Fetal liver cirrhosis and esophageal variceal hemorrhage in a patients with" Internal Medicine. 37. 1055-1057 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Kinoshita Y,et al: "Comparison of the signal transduction pathways activated by gastrin in" Gastroenterology. 115. 93-100 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Maekawa T,et al: "Reciprocal changes in progression of gastric MALT lymphoma and" Am J Med. 105. 539-541 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Fukui H,et al: "Reg protein may mediate gastric mucosal proliferation induced by" Gastroenterology. 115. 1483-1493 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Tanaka S,et al: "Laparoscopic fenestration in combination with ethanol sclerotherapy" Surg Laparosc Endosc. 8. 453-456 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Kazumori H,et al: "Primary sclerosing pancreatitis and cholangitis." International Journal of Pancreatology. 24. 123-127 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Kinoshita Y.et al: "Production and activation of hepatocyte growth factor" Digestion. 58. 225-231 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Ding M, et al: "Distribution of prostaglandin E receptors in the rat" Prostaglandins. 53. 199-216 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Kawanami C, et al: "Regenerating gene expression in normal gastric mucosa" J.Gastroenterol. 32. 12-18 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Morimoto K, et al: "Cellular localization of mRNAs for the prostaglandin E" Am.J.Physiol. 272. G681-G687 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Wang HY, et al: "Developmental gene expression of gastrin receptor" Regul Pep. 70. 183-189 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Maekawa T, et al: "Helicobacter pylori induced proinflammatory cytokines" J Lob Clin Med. 130. 442-449 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Kinoshita Y, et al: "Helicobacter pylori-independent chronological change of gastric acid" Gut. 41. 452-458 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Waki S, et al: "Sever muscle damage induced by higy carbohydrate" J.Gastroenterol. 33. 121-124 (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Waki S, et al: "Intragastric distribution of NSAID-related ulcers" J.Clin.Gastroenterology. 25. 592-594 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Kishi K, et al: "Pepsinogen C gene expression in gastritis" Biochem Biophys Res Commun. 238. 17-20 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Waki S, et al: "Effect of aging on gastrin receptor gene expression in rat stomach" Peptides. 19. 225-229 (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Yoshida M, et al: "Trichuris Trichiura infection" Am J Gastroenterol. 92. 719-720 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Hassan S, et al: "Expression of the proto-oncogene c-kit and its ligand" Dig.Dis.Sci. (in press).

    • Related Report
      1997 Annual Research Report
  • [Publications] Kinoshita Y, et al: "Increased hepatocyte growth factor content in rat stomach" Digestion. (in press).

    • Related Report
      1997 Annual Research Report
  • [Publications] Okada A, et al: "Rat gastric mucosal cells express ICAM-1and proinflammatory cytokines" J Lob Clin Med. (in press).

    • Related Report
      1997 Annual Research Report
  • [Publications] Hassan S, et al: "GGA,antiulcer drug,stimulates hexosamine production in a rat" J Gastroenterol Hepatol. (in press).

    • Related Report
      1997 Annual Research Report
  • [Publications] Maekawa T, et al: "Relation between severity and symptons of reflux esophagitis in elderly" J Gastroenterol Hepatol. (in press).

    • Related Report
      1997 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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