• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

IDENTIFICATION OF NUCLEAR PROTEIN EXPRESSED IN TRANSACTIVATED ITO CELLS

Research Project

Project/Area Number 09670554
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Gastroenterology
Research InstitutionKOCHI MEDICAL SCHOOL

Principal Investigator

SAIBARA Toshiji  Kochi Medical School, First Department of Internal Medicine, Assistant Professor, 医学部附属病院, 講師 (60145125)

Co-Investigator(Kenkyū-buntansha) UETA Hiroshi  Kochi Medical School, First Department of Internal Medicine, Research Associate, 医学部, 助手 (30284433)
Project Period (FY) 1997 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 1998: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1997: ¥2,000,000 (Direct Cost: ¥2,000,000)
KeywordsITO CELLS / LIVER / RAT / STELLATE CELLS / MYOFIBROBLAST / NITRIC OXIDE / CATIONIC AMINO ACID TRANSPORTER
Research Abstract

Development of liver fibrosis results in hepatic failure, hepatocellular carcinoma, esophageal varices, and spontaneous peritonitis, which are life-threatening complications in liver cirrhosis. Induction of apoptosis in transformed Ito cells maybe a promising way of treatment of chronic liver disease. A new insight into the changes of nuclear protein expressed in the process of transformation may provide an important information for the treatment of liver fibrosis.
Progress in purification of Ito cells enabled us to obtain highly-purified cells and we investigate the process of transformation precisely. In this study, we developed monoclonal antibody for purifying nuclear protein transiently expressed in transformed Ito cells during the process of transformation and the analysis of amino acid sequence revealed it as emelin commonly expressed in myocytes. We need further investigation to recognize the role of transient expression of emelin in Ito cells during transformation.

Report

(3 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • Research Products

    (24 results)

All Other

All Publications (24 results)

  • [Publications] Saibara T.et al: "Tanroxifen in early breast cancer" Lancet. 352. 404 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Ono M.et al.: "Transactivation of transforoning growth factor. α gene by hepatetis B virus pre SI." Cancer Res. 58. 1813-1816 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] K.Ookawauchi,et al.: "Characterization of cationic amino acid transporter and its gene expression in rat hepatic stellate cells in relation to nitric oxide production." J Hepatol. 29. 923-932 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Okumiya T.et al: "Sensitive enzymatic assay for erythrocyte creatine with production of methylene blue." Clin Chem. 44. 1489-1496 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Okumiya T.et al.: "Abnormally decreased HbA1c can be asses-sed with erythrocyte creatine in patients with a shortened erythrocyte age." Diabetes Care. 21. 1732-1735 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Jiao.Y,et al: "An enzymatic assay for erythrocyte creatine as an index of the erythrocyte life time" Clin Biochem. 31. 59-65 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Enzan H,et al: "Liver Diseases and Hepatic Sinusoidal Cells" Tanikawa K ,Ueno T eds.Springer, 347 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Saibara T, et al.: "Tamoxifen in early breast cancer" Lancet. 352. 404 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Ono M, et al.: "Transactivation of transforming growth factor-alpha gene by hepatitis B virus preS1." Cancer Res. 58. 1813-1816 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Ookawauchi K, et al.: "Characterization of cationic amino acid transporter and its gene expression in rat hepatic stellate cells in relation to nitric oxide production." J Hepatol. 29. 923-932 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Okumiya T, et al.: "Sensitive enzymatic assay for eruythrocyte creative with production of methylene blue." Clin Chem. 44. 1489-1496 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Okumiya T, et al.: "Abnormally decreased HbA1c can assessed with erythrocyte creatine in patients with a shortened erythrocyte age." Diabetes Care. 21. 1732-1735 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Jiso Y, et al.: "An enzymatic assay for erythrocyte creatine as an index of the erythrocyte life time." Clin Biochem. 31. 59-65 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Enzan H, et al.: Morphological aspects of hepatic fibrosis and Ito cells (Hepatic stellate cells), with special reference to their myofibroblastic transformation.Liver Diseases and Hepatic Sinusoidal cells (Tanikawa K, Ueno T eds.) Springer, (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Saibara T: el al: "Tanwxifen in early breast cancen." Lancet. 352. 404 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Ono M.et al.: "Transactivation of transforsning growth factor,α gene by hepatetis B virus presl." Cancer Res. 58. 1813-1816 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] K.Ookawauchi,et al.: "Characterization of cationic amino acid transporter and its gene expression in rat hepatic stellate cells in relation to nitric oxide production." J Hepatol. 29. 923-932 (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Okumiya T.el al.: "Sensitive enzymatic assay for erythrocyte creatine with production of methlene blue." Clin Chem. 44. 1489-1496 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Okumiya T.et al.: "Abnormally decreased HbA1c can be asses-sed with erythrocyte creatine in patients with a shortened erythrocyte age." Diabetes Care. 21. 1732-1735 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Jiao,Y.el al: "An enzymatic assay for erythrocyte creatine as an index of the erythrocyte life time" Clin Biochem. 31. 59-65 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Enzan H,et al: "Liver Diseases and Hepatic Sinusoidal Cells" Tanikawa K,Ueno T eds.Springer, 347頁 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 林芳弘 他: "分離・培養ラット肝伊東細胞の経時的微細構造変化" 肝類洞壁細胞研究の進歩. 10. 70-74 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 林芳弘 他: "肝線維化単内の間質細胞の微細構造" 肝類洞壁細胞研究の進歩. 9. 59-63 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Yamashita K et al: "A dimeric form of solecble recombinant sheep LFA3(CDS8)inhibits human T-cell proliferation by generating regulatory T cell S" Immunopharmacology. 37. 209-220 (1997)

    • Related Report
      1997 Annual Research Report

URL: 

Published: 1997-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi