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Role of the gastric mucus on the Helicobacter pylori infection.

Research Project

Project/Area Number 09670572
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Gastroenterology
Research InstitutionKITASATO UNIVERSITY

Principal Investigator

HOTTA Kyoko  Kitasato University School of Medicine, Professor, 医学部, 教授 (10050402)

Co-Investigator(Kenkyū-buntansha) KOIZUMI Wasaburou  Kitasato University School of Medicine, Assistant Professor, 医学部, 講師 (00195645)
OKAYASU Isao  Kitasato University.School of Medicine, Professor, 医学部, 教授 (20014342)
ICHIKAWA Takafumi  Kitasato University School of Medicine, Assistant Professor, 医学部, 講師 (30245378)
GOSO Yukinobu  Kitasato University School of Medicine, Assistant Professor, 医学部, 講師 (20112659)
ISHIHARA Kazuhiko  Kitasato Univ.Sch.Allied Health Sci., Professor, 医療衛生学部, 教授 (10104530)
Project Period (FY) 1997 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥2,700,000 (Direct Cost: ¥2,700,000)
Fiscal Year 1998: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1997: ¥1,900,000 (Direct Cost: ¥1,900,000)
KeywordsHelicobacter pylori / Mucus gel / Monoclonal antibody / Gastric mucus / mucin / Gastric mucosa / Dot-blot / ドットブロット
Research Abstract

The effects of the gastric mucus on Helicobacterpylori (Hp) infection have been studied.
1. The gastric mucins were purified by Lysine-Sepharose chromatography from the gastric juice which were obtained from the individuals having blood group A, B, AB or 0. The inhibition assay by the mucins in the red blood cell agglutination caused by Hp showed that the gastric mucins were potent inhibitor. The results indicated that Np bound to the gastric mucins obtained from the gastric juice. The sialydase treatment of the mucins caused the loss of the binding of the mucins to Hp ; therefore, the sialic acid residue was needed for the binding. The blood type was not correlate with the binding.
2. The gastric mucins were purified from the human gastric mucosa. The methods were authorized from the experiments using the rat gastric mucosa. The purified human gastric mucins were separated to the surface and glandular mucosa-specific mucins using the gel-filtration and ion-exchange chromatography. The dot-blot analysis and ELISA using monoclonal antibody HIK1083 were used to detect the individual mucin. The mucins from the surface mucosa were different from those from the glandular mucosa on their size. Although the complete separation of both mucins have not yet been done, the crude mucins bound to Hp. The mucins from the antrum bound strongly to Hp compared to those from the corpus.
3. The gastric mucins were prepared from the rat and mouse stomach and they were compared to the human mucins on the binding capacity to Hp. The rat or mouse gastric mucins bound only slightly to Hp. The differences of the structure among the mucins have been characterized.

Report

(3 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • Research Products

    (26 results)

All Other

All Publications (26 results)

  • [Publications] Hirmo S.他9名: "Inhibition of Helicobacter pylori sialic acid-specific haemagglutination by human gastrointestinal mucins and milk glycoproteins" FEMS Immunol. Med.Microbiol.20. 275-281 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Nakamura N.他6名: "Histochemical reactivity of normal, metaplastic, and neoplastic tissues to alpha-linked N-acetylglucosamine residue-specific monoclonal antibody HIK1083" J. Histochem. Cytochem.46. 793-801 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Ota H.他6名: "New monoclonal antibodies against gastric gland mucous cell-type mucins : a comparative immunohistochemical study" Histochem. Cell Biol.110. 113-119 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] T.Ichikawa他7名: "Distinct effects of tetragastrin, histamine, and CCh on rat gastric mucin synthesis and contribution of NO" Am.J.Physiol. 274. G138-G146 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Koizumi W. 他7名: "A prospective randomized study of amoxycillin and omeprazole with and without metronidazole in the eradication treatment of Helicobacter pylori" J.Gastroenterol. Hepatol.13. 301-304 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Goso Y.他5名: "Immortalized gastric epithelial cell line GSM06 synthesizes hyaluronan under the influence of simian virus 40 large T-antigen" J.Biochem.122. 96-100 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 堀田恭子、石原和彦: "胃粘液の魅力の探る-最新手法によるムチンの解明" メジカルビュー社, 98 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Hirmo S.et al.: "Inhibition of Helicobacter pylori sialic acid-specific haemaggluti-nation by human gastrointestinal mucins and milk glycoproteins" FEMS Immunol.Med.Microbiol.20. 275-281 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Nakamura N.et al.: "Histochemical reactivity of normal, metaplastic, and neoplastic to alpha-linked N-acetylglucosamine residue-specific monoclonal antibody HIK1083" J.Histochem.Cytochem. 46 (tissues). 793-801 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Ota H.et al.: "monoclonal antibodies against gastric gland mucous cell-type mucins : a comparative immunohistochemical study" Histochem.Cell Biol.110 (New). 113-119 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] T.Ichikawa et al.: "Distinct effects of tetragastrin, histamine, and CCh on rat gastric mucin synthesis and contribution of NO" Am.J.Physiol.274. G138-G146 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Koizumi W.et al.: "A prospective randomized study of amoxycillin and omeprazole with and without metronidazole in the eradication treatment of Helicobacter pylori" J.Gastroenterol. Hepatol.13. 301-304 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Yukinobu Goso et al.: "Immortalized gastric epithelial cell line GSM06 synthesizes hyaluronan under the influence of simian virus 40 large T-antigen" J.Biochem. 122. 96-100 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Y.Komuro 他4名: "Distinct effects of tetragastrin in rat gastroduodenal mucosa on mucin content and mucosal protective action against histamine-induced injury" Dig.Dis.Sci.42. 1050-1056 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Kurihara M.他6名: "Comparison of four monoclonal antibodies reacting with gastric gland mucous cell-derived mucins of rat and flog" Comp.Biochem.Physiol.121. 315-321 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Hirmo S.他9名: "Inhibition of Helicobacter pylori sialic acid-specific haemagglutination by human gastrointestinal mucins and milk glycoproteins" FEMS Immunol.Med.Microbiol.20. 275-281 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Goso Y.他5名: "Immortalized gastric epithelial cell line GSM06 synthesizes hyaluronan under the influence of simian virus 40 large T-antigen" J.Biochem.122. 96-100 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] T.Ichikawa 他7名: "Distinct effects of tetragastrin,histamine,and CCh on rat gastric mucin synthesis and contribution of NO" Am.J.Physiol.274. G138-G146 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Koizumi W.他7名: "A prospective randomized study of amoxycillin and omeprazole with and without metronidazole in the eradication treatment of Helicobacter pylori" J.Gastroenterol.Hepatol.13. 301-304 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 堀田恭子、石原和彦: "胃粘液の魅力を探る一最新手法によるムチンの解明" メジカルビュー社, 98 (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Y.Komuro他4名: "Distinct effects of tetragastrin in rat gastroduodenal mucosa on mucin content and mucosal protective action against histamine-induced injury" Dig.Dis.Sci.42(in press). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] T.Sakai他3名: "Recovery of mucin content in surface layer of rat gastric mucosa after HCl-aspirin-induced mucosal damage" J.Gastroenterol.32. 157-163 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] S.Ohara他2名: "Comparative study of carbohydrate portion of gastrointestinal mucins using enzyme-linked lectin-binding assay" Comp.Biochem.Physiol.116B. 167-172 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Y.Goso他5名: "Immortalized gastric epithelial cell line GSM06 synthesizes hyaluronan under the influence of simian virus 40 large T-antigen expression" J.Biochem.122. 96-100 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] T.Ichikawa他7名: "Distinct effects of tetragastrin,histamine,and CCh on rat gastric mucin synthesis and contribution of NO" Am.J.Physiol.274(in press). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] T.Ichikawa他3名: "Structural requirements for roxatidine in the stimulant effect of rat gastric mucin synthesis and the participation of nitric oxide in this mechanism" Br.J.Pharmacol.122. 1230-1236 (1997)

    • Related Report
      1997 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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