| Project/Area Number |
09670574
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Gastroenterology
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| Research Institution | Keio University |
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Principal Investigator |
IWAO .yasushi Keio University, School of Medicine, Assistant Professor, 医学部, 講師 (40168547)
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| Co-Investigator(Kenkyū-buntansha) |
MAKABE Toshiaki Keio University, Faculty of Science and Technology, Professor, 理工学部, 教授 (60095651)
HIBI Toshifumi Keio University, School of Medicine, Professor, 医学部, 教授 (50129623)
KUMAI Koichiro Keio University, School of Medicine, Associate Professor, 医学部, 助教授 (30101984)
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| Project Period (FY) |
1997 – 1999
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| Project Status |
Completed (Fiscal Year 1999)
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| Budget Amount *help |
¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 1999: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1998: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1997: ¥1,500,000 (Direct Cost: ¥1,500,000)
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| Keywords | autofluolescence / early colon cancer / LASER / 異型上皮 |
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| Research Abstract |
This study was designed to develop of a new method for detection of flat colon cancer and colitic cancer in which the recognition is difficult in the usual observation and contic cancer. 442nm He-Cd laser beam is irradiated to colonic mucosa under endoscopic observation using LIFE-Lung(Olympus Co.) as a laser scanning device, and then the autofluorescence was detected and amplified by a supersensitive camera. The change of energy metabolism and cellular structure was converted into the fluorescence spectrum. Colon cancer tissue show adarker red-brownish appearance in comparison to the bright green-yellow fluorescence of the normal mucosa on the monitor. Though there was a difference in adenoma and hyperplasia division at the fluorescent spectrum, the differentiation was not always easy. Furthermore, we examined mRNA expression specific to the chronic inflammation of ulcerative colitis and colon cancer, using the differential display method. Sixty of cDNA fragment was isolated, and subcloning and sequence were done on 34. The cDNA with homology in the IFN inducible gene 1-8U was isolated, and a fragment of about 150bp was isolated. However there was the expression of this gene both in colonic cancer and sporadic cancer, there was no expression of this gene in the normal or quiescent mucosa. It may become a marker which estimates the carcinogenic risk of ulcerative colitis.
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