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Analysis of Virus Genome in Sera from Patients with Silent Hepatitis B and Establishment of Detection Method for Antibodies against Virus

Research Project

Project/Area Number 09670580
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Gastroenterology
Research InstitutionJikei University School of Medicine

Principal Investigator

FUJISE Kiyotaka  Medical Department, Assistant Professor, 医学部, 助教授 (60057057)

Co-Investigator(Kenkyū-buntansha) NIIYA Minoru  Assistant, 医学部, 助手 (20198419)
Project Period (FY) 1997 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥2,900,000 (Direct Cost: ¥2,900,000)
Fiscal Year 1999: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1998: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1997: ¥1,600,000 (Direct Cost: ¥1,600,000)
KeywordsSilent hepatitis B / Hepatitis B virus / Hepatitis G virus / PCR method / Sequene analysis / Fulminant hepatitis / Precore mutation / PCR / シーケンス / HBVゲノム / プレコア領域 / 変異 / 非A-E型肝炎
Research Abstract

We studied 10 patients with acute hepatitis, including 2 cases of fulminant hepatitis, that was not drug-induced, alcoholic, or autoimmune and in which viral markers for hepatitis A-E were negative. Hepatitis G virus genome was not amplified in sera obtained from the 10 patients with RT-PCR at 5'-untranslted and helicase regions. Hepatits B virus (HBV) genome was amplified in 8 of the 10 patients, including 2 cases of fulminant hepatitis, with nested PCR at the precore region. Futhermore, we studied other 5 patients with fulminant hepatitis, in which viral markers for hepatitis A-E were negative. HBV genome was also amplified in 4 of the 5 patients with nested PCR at the same region. Amplified PCR products were analysed with the direct sequencing method. The point mutation that changes codon 28 (TGG) of the precore region to stop codon (TAG) was recognized in 4 of the 6 patiedts with fulminant hepatitis. Although HBV genome was not amplified with nested PCR at the X region in all patients,but amplified at the preS2 region in 3 of 6 patients with fulminant hepatitis. By using the precore and preS2 regions as a foothold, we tried to analyse the sequence of residual unknown region with the primer walking method. However, we cannot get the useful results so far to establish the detecction method for antibodies against the silent HBV.

Report

(4 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • 1997 Annual Research Report
  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] Kiyotaka FUJISE: "Hepatitis B virus variants in patients with acute hepatitis in whom various clinical forms develop"The Journal of the Japanese Association for Infections Diseases. 72. 67-74 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Kiyotaka FUJISE: "Detection of hepatitis B and G virus genomes in sera from patients with acute non-A, -B and-C hepatitis"Hepatology. 28. 218A (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 藤瀬清隆: "非A非B非C型劇症肝炎におけるSilent HBVの関与"日本消化器病学会雑誌. 96. A210 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 鈴木憲治: "B型急性肝炎劇症化例におけるHBV core promoter/pre-C, Core領域の変異に関する検討"東京慈恵会医科大学雑誌. 114. 497-506 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Kiyotaka FUJISE: "Hepatitis B virus variants in patients with acute hepatitis in whom various clinical forms develop"J Jpn Assn Infect Dis. 72-1. 67-74 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Kiyotaka FUJISE: "Detection of hepatitis B and G virus genomes in sera from patients with acute non-A, -B, and -C hepatitis"Hepatology. 28-4. 218 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Kiyotaka FUJISE: "Involvement of hepatitis B virus in fulminant non-A, -B, -C hepatitis"Jpn J Gastroenterol. 96-suppl.. 210 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Kenji SUZUKI: "Mutations in the core promoter, precore, and core regions of hepatitis B virus associated with fulminant hepatitis"Tokyo Jikeikai Med J.. 114-6. 497-506 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 藤瀬清隆: "非A非B非C型劇症肝炎におけるSilent HBVの関与"日本消化器病学会雑誌. 96・Suppl. 210 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 鈴木憲治: "B型急性肝炎劇症化例におけるHBV core promoter/Pre-C,Core領域の変異に関する検討"東京慈恵会医科大学雑誌. 114・6. 497-506 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] K.Fujise,et al.: "Detection of hepatitis B and G genomes in sera from patients with acute non-A,-B and -C hcpatitis" Hepatology. 28・4. 218A (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Kiyotaka FUJISE: "Hepatitis B virus variants in patients with acute hepatitis in whom various clinical forms develop" The journal of the japanese association for infections diseases. 72・1. 67-74 (1998)

    • Related Report
      1997 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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