| Project/Area Number |
09670584
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Gastroenterology
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| Research Institution | St.Marianna University School of Medicine |
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Principal Investigator |
SUZUKI Michihiro St.Marianna University School of Medicine, The Medical Department, Lectures., 医学部, 講師 (50187770)
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| Co-Investigator(Kenkyū-buntansha) |
SUEMORI Shoiichi St.Marianna University School of Medicine, The Medical Department, Lectures., 医学部, 講師 (90235838)
SUZUKI Hiroshi St.Marianna University School of Medicine, The Medical Department, Assistant Pro, 医学部, 助教授 (60081692)
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| Project Period (FY) |
1997 – 1998
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| Project Status |
Completed (Fiscal Year 1998)
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| Budget Amount *help |
¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 1998: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1997: ¥2,300,000 (Direct Cost: ¥2,300,000)
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| Keywords | heaptocellular carcinoma / carbohydrate analysis / urinary levels of free L-fucose / glycoprotein / oligosaccharide / Fluorophore Assisted Carbohydrate Electrophoresis(FACE) / 肝癌細胞 / 複合糖蛋白 / 糖鎖配列 |
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| Research Abstract |
Glycolipides and antiglycolipid antibodies were measured in serum and tissue of hepatocellular carcinoma(HCC) . These results suggested that characteristic spectrum of glycolipides and antiglycolipid antibodies existed in HCC.
We studied that the efficiency of gangliosides for tumor cell adhesion and interaction. Gangliosides inhibited of extra cellular matrix (Laminin and Fibronectin) for tumor cell adhesion. But gangliosides were not clear effect in proliferation of tumor cell growth.
We measured urinary levels of free L-fucose(UFC) in HCC.Advanced stage of HCC had higher levels of UFC than early stage of HCC.However, the levels of UFC elevated with progression of clinical stage of HCC, also correlated well with serum albumin and choline esterase. These results suggested that UFC was not a tumor marker appropriate to diagnose for HCC.
We detected oiigosaccharides from glycoproteins in urine and tissue of HCC, using Fluorophore Assisted Carbohydrate Electrophoresis(FACE). The results of urinary electrophoretic profiles of normal, liver cirrhosis and HCC were compared. In HCC cases, there were some characteristic bands inlevel of upper G4 and strong intensity were hardly detected in normal regions(under G4). Also the electrophoretic profiles of cancerous tissue of HCC was characteristic, compared with non cancerous tissue These results suggested that some oligosaccharides might be specific to HCC.
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