| Project/Area Number |
09670602
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Respiratory organ internal medicine
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| Research Institution | University of Tokyo |
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Principal Investigator |
OKABE Taturo University of Tokyo Faculty of Medicine Assistant, 医学部・附属病院, 助手 (80169135)
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| Project Period (FY) |
1997 – 1998
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| Project Status |
Completed (Fiscal Year 1998)
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| Budget Amount *help |
¥2,900,000 (Direct Cost: ¥2,900,000)
Fiscal Year 1998: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1997: ¥1,800,000 (Direct Cost: ¥1,800,000)
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| Keywords | endothelial cell / growth factor / insulin-like growth factor-II / lung cancer / tumor / leukomia |
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| Research Abstract |
It is well recognized that tumor growth is dependent on the development of new blood vessels (angiogenesis). Many tumor cells produce growth factors of vascular endothelial cells. Such growth factors include basic fibroblast growth factor (FGF), acidic FGF, vascular endothelial growth factor (VEGF), and transforming growth factor-alpha (TGF-alpha). Acidic FGF, basic FGF and VEGF have affinity for heparin and bind to heparin sepharose affinity columns. We observed that the conditioned medium from a human lung cancer cell line T3M-11 was mitogenic for endothelial cells derived from porcine aorta. The activity did not bind to a heparin sepharose affinity column and was different from TGF-alpha immunologically. These findings led us to analyze the growth factor produced by T3M-11 cells.
A cDNA encoding the growth factor was isolated through functional expression cloning in COS-1 cells from a cDNA library prepared from T3M-11 cells. The nucleotide sequence encoded by the cDNA proved to be identical with insulin-like growth factor II (IGF-II). The result suggests the involvement of IGF-II in the angiogenesis induced by the malignant tumor cells.
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