| Project/Area Number |
09670617
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Respiratory organ internal medicine
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| Research Institution | Kagawa Medical university |
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Principal Investigator |
FUJITA Jiro Kagawa Medical University, 1st Dept Int Med, Research Associate, 医学部, 助手 (80209056)
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| Co-Investigator(Kenkyū-buntansha) |
OKADA Hiroki Kagawa Medical University, University Hospital, Lecturer, 医学部・附属病院, 講師 (00243775)
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| Project Period (FY) |
1997 – 1999
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| Project Status |
Completed (Fiscal Year 1999)
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| Budget Amount *help |
¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 1999: ¥100,000 (Direct Cost: ¥100,000)
Fiscal Year 1998: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1997: ¥2,000,000 (Direct Cost: ¥2,000,000)
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| Keywords | Arbitrarily-primed PCR / MRSA / Typing / Nosocomial infection |
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| Research Abstract |
In 1997, we established the typing method for methicillin-resistant Staphylococcus aureus (MRSA) by an arbitrarily-primed polymerase. Chain reaction. In 1998, we applied this method to several strains of MRSA isolated in Kagawa Medical University Hospital. Usefulness of the typing method by AP-PCR was compared to other typing methods: phenotypic characteristics (production of enterotoxin and TSST-1, antimicrobial susceptibity) and molecular typing procedure (plasmid DNA profile, pulsed-field gel electrophoresis [PFGE] . Although traditional epidemiological methods for example, coagulase typing plays a central role in hospital infection control, combination of plasmid DNA profile, AP-PCR, and PFGE may prove to be a particularly informative means of tracking the nosocomial spread of MRSA. Using AP-PCR, we could prove the nosocomial infection caused by cagulase VII positive MRSA strain .
In 1999, we applied this method to other bacteria : Pseudomonas cepacia, Pasteurella multocida, and Mycobacterium avium intracellulare complex, and we demonstrated that AP-PCR could be a useful and simple method to investigate the transmission of several bacteria.
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