| Project/Area Number |
09670643
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Neurology
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| Research Institution | Tokyo Medical and Dental University |
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Principal Investigator |
FURUKAWA Tetsuo Tokyo Medical and Dental University School of Medicine Professor, 医学部, 教授 (80134667)
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| Co-Investigator(Kenkyū-buntansha) |
YAMADA Masahito Tokyo Medical and Dental University Graduate School Associate professor, 大学院・医歯学総合研究科, 助教授 (80191336)
鎌田 智幸 東京医科歯科大学, 医学部, 助手
和田 義明 東京医科歯科大学, 医学部, 助手 (50182986)
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| Project Period (FY) |
1997 – 1999
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| Project Status |
Completed (Fiscal Year 1999)
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| Budget Amount *help |
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1999: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1998: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1997: ¥1,000,000 (Direct Cost: ¥1,000,000)
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| Keywords | Malignant lymphoma / Peripheral neuropathy / Peripheral nerve myelin / Major myelin protein zero / Monoclonal anti-P0 antibody / Isoform / 末梢性ミエリン / ゲル電気泳動 / ミエリン / 馬尾 / ウエスタンブロット / 可溶化 / 界面活性剤 / 二次元電気泳動 / 坐骨神経 / mRNA / RACE / ノーザンブロットハイブリダイゼーション / cDNAライブラリー / プラークハイブリダイゼーション |
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| Research Abstract |
We tried to characterize a 35-kd antigen recognized by the serum IgG of a patient with malignant lymphoma and peripheral neuropathy. On western blotting, the serum IgG reacted with a 35-kd antigen in human, bovine and mouse peripheral nerves (PN) but not with other neural and non-neural tissues. Immunohistochemical analysis showed immunoreactivity for the IgG in the compact myelin of PN. We constructed a human sciatic nerve cDNA library and screened it using the patient's IgG. We identified three independent clones. A homology search of the inserts of these clones revealed that the inserts were homologous to P0 cDNA. However, all the inserts corresponded to the 3'-untranslational region of P0 cDNA. Then, to analyze the 35-kd antigen biochmically, myelin fractions of human and bovine sciatic nerve were prepared. Using SDS-polyacrylamide gel electrophresis, the 35-kd antigen was purified from the crude myelin fraction. When the immunoreactivities of the 35-kd antigen for the patient's IgG and monoclonal anti-P0 antibody were compared with those of protein P0 for these antibodies, the 35-kd antigen reacted with both the antibodies, but P0 reacted with only monoclonal anti-P0 antibody. These results indicate the possibility of the 35-kd antigen being an isoform of P0. However, the presence of these autoantibodies against the 35-kd antigen seems to be of little pathological significance, because circulating autoantibodies against the antigen were also found in the sera of patients with malignant lymphoma without associated peripheral neuropathy.
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