| Project/Area Number |
09670645
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Neurology
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| Research Institution | Niigata University |
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Principal Investigator |
INUZUKA Takashi Niigata University Medical Hospital Lecturer, 医学部附属病院, 講師 (50184734)
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| Co-Investigator(Kenkyū-buntansha) |
NAKANO Ryouichi Niigata University Medical Hospital Assistant, 医学部附属病院, 助手 (00262444)
HOZUMI Isao Niigata University Medical Hospital Assistant, 医学部附属病院, 助手 (20242430)
TANAKA Keiko Niigata University Medical Hospital Assistant, 医学部附属病院, 助手 (30217020)
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| Project Period (FY) |
1997 – 1998
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| Project Status |
Completed (Fiscal Year 1998)
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| Budget Amount *help |
¥2,600,000 (Direct Cost: ¥2,600,000)
Fiscal Year 1998: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1997: ¥2,000,000 (Direct Cost: ¥2,000,000)
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| Keywords | anti-neural antibody / malignancy / paraneoplastic neurological syndrome / RNA recognition motif / neuronal cell death / anti-sense oligonucleotide / 腫瘍マーカー / 自己免疫 |
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| Research Abstract |
We established the detection system for anti-neural antibodies, using immunoblot, immunohistochemical approach and ELISA.Over 300 samples were examined in 1997-98. Thirty percent of those contained auto-antibodies, one-third of it reacted with neural antigens.
We could not find any characteristic band or pattern of immunostaining in some specific neurological disease except some known auto-antibodies such as anti-Hu, anti-Yo and anti MAG antibodies.
We studied clinical analysis and characterization of anti-nuclear antibodies in 21 patients of paraneoplastic neurological syndrome with anti-Hu antibodies. Clinical features were variable regarding anti-Hu positive PNS, such as sensory neuropathy. Small cell lung cancer was often associated with anti-Hu positive PNS.Detection of anti-Hu antibodies suggest some specific kinds of occult malignancy and are helpful when searching for malignancy. These patients often have nuclear antibodies in addition to anti-Hu antibodies. As recognized antigens by anti-Hu antibodies contain RNA recognition motif, we examined effects of anti-Hu antibodies using culture system of PC12 cell. Anti-Hu antibodies were added in culture medium or injected into the cell by micro injector, Significant cell injury were not observed. We found the inhibition effect of HuD anti-oligonucleotide but not anti-Hu antibodies on presentation of HuD protein and differentiation of PC12 to neuronal cell adding of NGF.HuD protein may relate to differentiation.
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