| Project/Area Number |
09670674
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Neurology
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| Research Institution | Tokyo Women's Medical School |
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Principal Investigator |
OHA Kohei Tokyo Women's Medical University, Dept. of Neurology, 医学部, 講師 (00152132)
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| Co-Investigator(Kenkyū-buntansha) |
AKIYAMA Naoko Tokyo Women's Medical School, Dept. of Neurology, 医学部, 助手 (00256588)
SHIMIZU Yuki Tokyo Women's Medical School, Dept. of Neurology, 医学部, 助手 (20246507)
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| Project Period (FY) |
1997 – 1999
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| Project Status |
Completed (Fiscal Year 1999)
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| Budget Amount *help |
¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 1999: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1998: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1997: ¥1,500,000 (Direct Cost: ¥1,500,000)
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| Keywords | Vα24+T cell / Natural killer T cell / Th1 cells / Th2 cells / Multiple sclerosis / Chronic inflammatory demyelinating polyneuropathy / Gullain-Barre Syndrome / Immunoadsorption therapy / multiple sclerosis / immune regulation / NKT cell / leulcocyte activation / immunoadsorbent therapy / chronic inflammatory demyelirating polyneuropathy / T helper 1 cell / T helper 2 cell / immuno adosorbent therapy / Mac-1 / L-selectin / myelin basic protein / anti-ergotypic T cell / anti clouotypic T cell / immune vegulation |
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| Research Abstract |
1) Vα24+ regulatory T cell in inflammatory demyelinating disease
Vα+24T cells produced IFN-γ and IL-4 may have regulary mechanism, we therefore measured positive percentage of peripheral blood Vα24+T cells in patient with neuroimmunological disorders. A positive percentage of Vα24+T cells in multiple sclerosis(MS), chronic inflammatory demyelinating polyneuropathy (CIDP) and Gullain-Barre syndrome was not significant however fine analysis by double stain of anti- Vα24 and Vβ11 antibodies and further examinations considered the stage or treatment of a patient may necessary to determine the role of Vα24+T cells in inflammatory demyelinating diseases.
2) Immunoregulatory cytokine producing T cell in inflammatory demyelinating disease
We Examined IFN-γ and IL-4+T cells in inflammatory demyelinating diseases because the regulatory mechanism between Th1 and Th2 cells takes important role in experimental auto immune encephalomyelitis. The positive percentage of IFN-γ+CD4 cell in the exacerbation
… More
of MS showed a tendency of increase and decrement of IFN-γ+CD4 cell was noted in some MS patients after steroid therapy. It was suggested the regulatory mechanism between Th1 and Th2 cells might be changed by the condition of a MS patient.
3) IL-2 reactive natural killer (NK) γδT cell in MS
Peripheral blood γδT cells associated with CD56 may play a regulatory role in the disease because they are sensitive and to IL-2 and sometimes take a high value in MS patiensts. When we examined dynamics of NKγδT cells in MS patients to determine their importance in the immune response, the variation of positive percentage and cytotoxicity of NKγδT cells was recognized by a stage of a disease. Thus, the possibility that NKγδT cell might influence to the pathogenesis of MS was suggested.
4) Neutrophil activation in immunoadsorption
To define the influence of Immunoadsorption therapy (IAT) on leukocytes, we determined leukocytes L-selectin and Mac-1 during CIDP during IAT. Expression of neutophils (PMN) L-selctin was significantly decreased and PMN Mac-l was markedly increased by IAT, while expression of mononuclear cells (MNC) L-selectin slightly increased during IAT and MNC Mac-1 was not changed during IAT. L-selectin downregulation and Mac-1 upregulation on PMN suggested that activation of PMN associated with changes in peripheral leukocytes distribution occurred during IAT and might play some role in modulating immune system. Less
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