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Cloning of Neuron Specific Protein, MxR

Research Project

Project/Area Number 09670680
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Neurology
Research InstitutionFukuoka University

Principal Investigator

TSUBOI Yoshio  Fukuoka Univ., Sch. of Medicine. Assist. Prof., 医学部, 助手 (90291822)

Co-Investigator(Kenkyū-buntansha) TOYODA Tetsuya  Kurume Univ., Sch. of Medicine. Assist. Professor, 医学部, 教授 (00197972)
YAMADA Tatsuo  Fukuoka Univ., Sch. of Medicine. Assist. Professor, 医学部, 教授 (60159217)
Project Period (FY) 1997 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 1999: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1998: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1997: ¥2,000,000 (Direct Cost: ¥2,000,000)
KeywordsEndoplasmic reticulum / Neuron / Neuroendocrine specific protein / Reticulon / cDNA cloning / FISH / Mx-1 / マウス / 核 / 軸原 / シナクロス / 軸策 / シナプシス
Research Abstract

We cloned a cDNA encoding mouse neuroendocrine specific protein/reticulon (RTN) 3 by screening mice brain cDNA library. The cDNA of mRTN3/mMxR1 was 1745nts and contains 711nts or open reading frame (ORF). It has 832nls of long 3' noncoding region (NCR), and poly A signal (AATAAA) is located at l694. The sequence analysis indicated that mRTN3/mMxR1 has strong homology with human RTN in its C-terminus. Northern blot analysis indicated that mRTN3/mMxR1 was expressed in brain. Its expression was not controlled embryonic-developmentally. We expressed the ORF as a fusion protein with green fluorescent protein in COS 7 or HeLa cells. The expressed fusion protein was localized in the endoplasmic reticulum. When the unique N-terminal sequence was removed, the truncated fusion protein remained in the ER though the intensity of the protein was reduced. We are now trying to determine the topology of mRTN3/mMxR1 to the ER membrane. We are also trying to make a specific antibodies by immunizing rabbits with synthetic peptides.

Report

(4 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • 1997 Annual Research Report
  • Research Products

    (4 results)

All Other

All Publications (4 results)

  • [Publications] Kawaguchi et al.: "Rare tevelency of catching cold in Parkinson's disease"Parkinsonism and Related disordes. 4. 207-209 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Kawaguchi et al.: "Expression of MxA mRNA in peripheal blood cells in Parkinson's disease"Parkinsonism and Related disorders. 5. 43-47 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Kawaguchi et al: "Rare tendency 'of catching cold in Parkinson's disease."Parkinsonism and Related Disorders. 4. 207-209 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Kawaguchi et al: "Expression of MxA mRNA in peripheral blood mononuclear cells in Parkinson's disease."Parkinsonism and Related Disorders. 5. 43-47 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary

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Published: 1997-04-01   Modified: 2016-04-21  

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