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Analysis of experimental autoimmune myositis and its immunotherapy

Research Project

Project/Area Number 09670682
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Neurology
Research InstitutionTokyo Metropolitan Institute for Neuroscience

Principal Investigator

KOJIMA Takashi  Tokyo Metropolitan Institute for Neuroscience, 神経病理研究部門, 主事研究員 (30225429)

Co-Investigator(Kenkyū-buntansha) KAWAZOE Yoko  Tokyo Metropolitan Institute for Neuroscience, 神経病理研究部門, 主事研究員 (60281705)
TANUMA Naoyuki  Tokyo Metropolitan Institute for Neuroscience, 神経病理研究部門, 研究員 (00281676)
MATSUMOTO Yoh  Tokyo Metropolitan Institute for Neuroscience, 神経病理研究部門, 副参事研究員 (90173921)
Project Period (FY) 1997 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥2,800,000 (Direct Cost: ¥2,800,000)
Fiscal Year 1998: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 1997: ¥1,500,000 (Direct Cost: ¥1,500,000)
Keywordspolymyositis / myositogenic T cells / T cell receptor / CDR3 spectrayping / DNA vaccine
Research Abstract

In order to know the pathogenesis of human polymyositis, experimetal autoimmune myositis (EAM) was induced in Lewis rats by immunization with partially purified myosin and the nature of T cell receptor (TCR) of EAM-inducing T cells was analyzed by CDR3 spectratyping. It was revealed that TCR Vbeta8.2, 10 and 12 of muscle-infiltrating T cells was oligoclonally expanded during the course of EAM.We are currently investigating whether pretreatment with DNA vaccines which are prepared by inserting full length of these Vbetas into the expression vector suppresses the development of EAM.
We have also tried to identify the major myositogenic antigen in the partially purified myosin preparation. For this purpose, partially purified myosin was further purified by the chromatographic techniques into purified myosin and C-protein. Immunization with t C-protein induced severe EAM, whereas that with purified myosin produced only mild lesions in the muscle. This finding indicates that C-protein, but not myosin, is the major myositogenic antigen.

Report

(3 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • Research Products

    (27 results)

All Other

All Publications (27 results)

  • [Publications] T.Kohji et al.: "Interaction between apoptotic cells and reactive brain cells in the central nervous system of rats with autoimmune encephalomyelitis" J.Neuroimmunol.82. 168-174 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] G.Kim et al.: "CDR3 size spectratyping and sequencing of spectratype-derived T cell receptor of spinal cord T cells in autoimmune encephalomyelitis" J.Immunol.160. 509-513 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] K.Kogure et al.: "Quantitative analysis of pro-and anti-inflammatory cytokine mRNA in neural graft rejection" J.Neurommunol.87. 114-120 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Y.Aikawa et al.: "A new anti-rheumatic agent,3-formylamino-7-methylsulfonylamino-6-phenoxy-4H-1-benzopyran-4-one(t-614),effectively suppresses the development of autoimmune encephalomyelitis" J.Neurommunol.89. 35-42 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Y.Matsumoto et al.: "Role of natural killer cells and TCRγδ T cells in acute autommune encephalomyelitis" Eur.J.Immunol.28. 1681-1688 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] G.Kim et al.: "Persistent expression of autoimmune encephalomyelitis (EAE)-specific Vβ8.2 TCR spectratype in the central nervous system of rats with chronic relapsing EAE" J.Immunol.161. 6993-6998 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] G.Kim, K.Kohyama, N.Tanuma, H.Arimto, Y.Matsumoto: "Persistent expression of autoimmune encephalomyelitis (EAE)-specific Vbeta8.2 TCR spectratype in the central nervous system of rats with chronic relapsing EAE." J.Immunol.161. 6993-6998 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] T.Shin, J.Jin. N.Tanuma, K.Kohyama, K.Kim, Y.Matsumoto. B.Hyun: "Inhibition of inducible nitric oxide synthase ameliorates experimental autoimmune myocarditis in Lewis rats." J.Neuroimmunol.28. 2751-2759 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] G.Kim, K.Kohyama, N.Tanuma, Y.Matsmoto: "Stage-dependent usage of TCR Valpha chain with different CDR3 motifs by spinal cord T cells in autoimmune encephalomyelitis." J.Neuroimmunol.(in press). (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] G.Kim, N.Tanuma, T.Kojima, K.Kohyama, Y.Suzuki, Y.Kawazoe, Y.Matsumoto: "CDR3 size spectratyping and sequencing of spectratype-derived T cell receptor of spinal cord T cells in autoimmune encephalomyelitis." J.Immunol.160. 509-513 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] K.Kogure, N.Tanuma, A.Teramoto, Y.Matsumoto.: "Quantitative analysis of pro- and anti-inflammatory cytokine mRNA in neural graft rejection." J.Neuroimmunol.87. 114-120 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] G.Kim, K.Kohyama, N.Tanuma, Y.Matsumoto: "Diagnosis and assessment of preclinical and clinical autoimmune encephalomyelitis using peripheral blood TCR." Eur.J.Immunol.28. 2751-2759 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] T.Kohji, N.Tanuma, Y.Aikawa, T.Kojima, Y.Matsumoto: "Interaction between apoptotic cells and reactive brain cells in the central nervous system of rats with autoimmune encephalomyelitis." J.Neuroimmunol.82. 168-174 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Y.Aikawa, N.Tanuma, T.Shin, T.Kojima, S.Makino, K.Tanaka, Y.Matsumoto: "A new anti-rheumatic agent, 3-formylamino-7-methylsulfonylamino-6-phenoxy-4H-1-benzopyran-4-one (T-614) , effectively suppresses the development of autoimmune encephalomyelitis." J.Neuroimmunol.89. 35-42 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Y.Matsumoto, Y.Kohyama, Y.Aikawa, T.Shin, Y.Kawazoe, Y.Suzuki, N.Tanuma: "Role of natural killer cells and TCRUPSILONdelta T cells in acute autoimmune encephalomyelitis." Eur.J.Immunol.28. 1681-1688 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] T.Kohji et al.: "Interaction between apoptotic cells and reactive brain cells in the central nervous system of rats with autoimmune encephalomyelitis" J.Neuroimmunol.82. 168-174 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] G.Kim et al.: "CDR3 size spectratyping and sequencing of spectratype-derived T cell receptor of spinal cord T cells in autoimmune encephalomyelitis" J.Immunol.160. 509-513 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] K.Kogure et al.: "Quantitative analysis of pro- and anti-inflammatory cytokine mRNA in neural graft rejection" J.Neuroimmunol.87. 114-120 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Y.Aikawa et al.: "A new anti-rheumatic agent, 3-formylamino-7-methylsulfonylamino-6-phenoxy-4H-1-benzopyran-4-one(T-614), effectively suppresses the development of autoimmune encephalomyelitis" J.Neuroimmunol.89. 35-42 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Y.Matsumoto et al.: "Role of natural killer cells and TCRγδ T cells in acute autoimmune encephalomyelitis" Eur.J.Immunol.28. 1681-1688 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] G.Kim et al.: "Persistent expression of autoimmune encephalomyelitis(EAE)-specific Vβ8.2 TCR spectratype in the central nervous system of rats with chronic relapsing EAE" J.Immunol.161. 6993-6998 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] T.Kohji et al.: "Interaction between apoptotic cells and reactive brain cells in the central nervous system of rats with autoimmune encephalomyelitis" J.Neuroimmunol.(in press). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] G.Kim et al.: "CDR3 size spectratyping and sequencing of spectratype-derived T cell receptor of spinal cord T cells in autoimmune encephalomyelitis" J.Immunol.160. 509-513 (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] T.Kojima et al.: "Myosin-induced autoimmune polymyositis in the rats." J.Neurol.Sci.151. 141-147 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Matsumoto,Y.et al.: "Characterization of CD4-CD8- TCRab+ T cells appearing in the subarachnoid space of rats with autoimmune encephalomyelitis." Eur.J.Immunol.26. 1328-1334 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] Tanuma,N.et al.: "Pretreatment with T cell receptor peptide using conventional immunization protocol does not induce effective protection against autoimmune" Cell.Immunol.168. 85-90 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] Shin,T.et al.: "The subarachnoid space as a site for precursor T cell proliferation and effector T cell selection in experimental autoimmune encephalomyelitis." J.Neuroimmunol.56. 171-177 (1995)

    • Related Report
      1997 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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