| Project/Area Number |
09670682
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Neurology
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| Research Institution | Tokyo Metropolitan Institute for Neuroscience |
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Principal Investigator |
KOJIMA Takashi Tokyo Metropolitan Institute for Neuroscience, 神経病理研究部門, 主事研究員 (30225429)
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| Co-Investigator(Kenkyū-buntansha) |
KAWAZOE Yoko Tokyo Metropolitan Institute for Neuroscience, 神経病理研究部門, 主事研究員 (60281705)
TANUMA Naoyuki Tokyo Metropolitan Institute for Neuroscience, 神経病理研究部門, 研究員 (00281676)
MATSUMOTO Yoh Tokyo Metropolitan Institute for Neuroscience, 神経病理研究部門, 副参事研究員 (90173921)
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| Project Period (FY) |
1997 – 1998
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| Project Status |
Completed (Fiscal Year 1998)
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| Budget Amount *help |
¥2,800,000 (Direct Cost: ¥2,800,000)
Fiscal Year 1998: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 1997: ¥1,500,000 (Direct Cost: ¥1,500,000)
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| Keywords | polymyositis / myositogenic T cells / T cell receptor / CDR3 spectrayping / DNA vaccine |
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| Research Abstract |
In order to know the pathogenesis of human polymyositis, experimetal autoimmune myositis (EAM) was induced in Lewis rats by immunization with partially purified myosin and the nature of T cell receptor (TCR) of EAM-inducing T cells was analyzed by CDR3 spectratyping. It was revealed that TCR Vbeta8.2, 10 and 12 of muscle-infiltrating T cells was oligoclonally expanded during the course of EAM.We are currently investigating whether pretreatment with DNA vaccines which are prepared by inserting full length of these Vbetas into the expression vector suppresses the development of EAM.
We have also tried to identify the major myositogenic antigen in the partially purified myosin preparation. For this purpose, partially purified myosin was further purified by the chromatographic techniques into purified myosin and C-protein. Immunization with t C-protein induced severe EAM, whereas that with purified myosin produced only mild lesions in the muscle. This finding indicates that C-protein, but not myosin, is the major myositogenic antigen.
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