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Establishment of nitric oxide measurement in exhaled air and its clinical application.

Research Project

Project/Area Number 09670700
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Circulatory organs internal medicine
Research InstitutionUniversity of Tokyo

Principal Investigator

HIRATA Yasunobu (1998)  Univ. of Tokyo, Internal Med. , Lecturer, 医学部・附属病院, 講師 (70167609)

百村 伸一 (1997)  東京大学, 医学部附属病院, 助手 (10190985)

Co-Investigator(Kenkyū-buntansha) MOMOMURA Shin-ichi  Toranomon Hospital, Internal Med. , Chief, 沖中記念研究所, 部長 (10190985)
TAKENAKA Katsu  Univ. of Tokyo, Internal Med. , Lecturer, 医学部・附属病院, 講師 (20188204)
SUGIURA Seiryo  Univ. of Tokyo, Internal Med. , Assist. Prof., 医学部・附属病院, 助手 (10272551)
AOYAGI Teruhiko  Univ. of Tokyo, Internal Med. , Assist. Prof., 医学部・附属病院, 助手 (10251240)
MATSUMOTO Akihiro  Univ. of Tokyo, Internal Med. , Clin. fellow, 医学部・附属病院, 医員
横山 郁夫  東京大学, 医学部附属病院, 医員
平田 恭信  東京大学, 医学部附属病院, 講師 (70167609)
Project Period (FY) 1997 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥2,800,000 (Direct Cost: ¥2,800,000)
Fiscal Year 1998: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 1997: ¥1,500,000 (Direct Cost: ¥1,500,000)
KeywordsNitric Oxide / Exhaled gas / plasma NOx / Exercise test / hypertension / hyper lipidemia / diabetes mellitus / renal disease / 慢性心不全 / 慢性閉寒性肺疾患
Research Abstract

This study was performed to establish nitric oxide(NO) measurement iii the exhaled air in humans and to assess whether endogenous NO production of the lungs is abnormally regulated at rest amid during exercise in patients with various kinds of diseases such as congestive heart failure (CHF), hypertension (HT), hypercholesteremia (HL), diabetes mellitus (DM), lung diseases, chronic glomerular nephritis (CGN), and chronic renal failure (CRF). Plasma concentrations or nitrite plus nitrate (NOX) were measured by lime Griess method as indicators of systemic NO production.
1) Patients with CHF (n=14) had higher exhaled NO concentration and output than controls. Exercise increased NO output about 3 times in controls, but the increase was smaller in the patients. These findings suggest that abnormal regulation of NO production may cause the attenuated exercise-induced vasodilation iii heart failure.
2) Patients with HT, HL, and DM had the similar levels of exhaled NO concentration amid output co … More mpared with normal controls at rest. Although exercise increased NO output in both groups, the increase was less in these patients than was normals. Plasma NOX concentrations in the normals increased during exercise, but not in patients with HT.These findings suggested that exercise-induced increase of NO production, most likely as a consequence of increased shear stress, was impaired in these patients.
3) Patients with lung diseases such as idiopathic pneumonitis and collagen diseases, had a higher exhaled NO concentration amid output. These results suggest that endogenous NO production is increased in the lungs of patients with mining imflamation of collagen diseases, and it may play some pathophysiological roles in these patients.
4) Patients with CGN (n=21) exhaled higher concentrations of NO and output than did controls. Plasma NOX concentrations were also significantly greater in the patients than in the controls. In patients with CGN, exhaled NO output correlated negatively with creatinine clearance. These findings suggested that endogenous NO production is increased in patients with CGN.Increased endogenous NO production may play sonic pathophysiological role in these patients.
5) Patients with CRE (n=40) had a higher exhaled NO concentration and output, and a higher plasma NOX concentration than did controls. These values did not differ between patients on hemodialysis amid those on continuous ambulatory peritoneal dialysis. The increase in exhaled NO with the simultaneous increase in circulating cytokines suggests that NO synthase seems to be induced significantly in these patients. increased endogenous NO production may have sonic pathophysiological role in uremic patients. Less

Report

(3 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • Research Products

    (24 results)

All Other

All Publications (24 results)

  • [Publications] Nakatsubo N,Kojima H,他: "Direct evidence of nitric oxide production from bovine aortic endothelial cells using new fluorescence indicators : diaminofluoresceins." FEBS Lett. 427. 263-266 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kojima H,Nakatsubo N,他: "Detection and imaging of nitric oxide with novel fluorescent indicators : diaminofluoresceins." Anal Chem. 70. 2446-2453 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Matsumoto A,Hirata Y,他: "Increased excretion of nitric oxide in exhaled air of patients with chronic renal failure." Clin Sci. 96. 67-74 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Matsumoto A,Momomura S,他: "The effect of nitric oxide on gas exchange in patients with congestive heart failure." Ann Intern Med. 130. 40-44 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kakoki M,Hirata Y,他: "Effects of vasodilatory β-adrenoceptor antagonists on endothelium-derived nitric oxide release in rat kidney." Hypertension. 33. 467-471 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kakoki M,Matsumoto A,他: "Analysis of nitric oxide in the exhaled air of patients with chronic glomerulonephritis." Clin Nephr. in press.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Nakatsubo N,Kojima H,Kikuchi K,Nagoshi H,Hirata Y,Maeda D,Imai Y,Irimura T,Nagano T: "Dircct evidence of nitric oxide production from bovine aortic endothelial cells using new fluorescence indicators : diaminofluoresceins." FEBS Lett. 127. 263-266 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kojima H,Nakatsubo N,Kikuchi K,Kawahara S,Kirino Y,Nagoshi H,Hirata Y,Nagano T: "Detection and imaging of nitric oxide with novel fluorescent indicators : diaminofluoresceins." Anal Chem. 70. 2446-2453 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Matsumoto A,Hirata Y,Kakoki M,Nagata D,Momomura S,Sugimoto T,Tagawa H,Omata M: "Increased Excretion of Nitric Oxide in Exhaled Air of Patients With Chronic Renal Failure" Clin Sci. 96. 67-64 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Matsumoto A,Momomura S,Sugiura S,Fujita H,Aoyagi T,Sala M,Omata M,Hirata Y: "The effect of nitric oxide on gas exchange in patients with congestive heart failure." Ann Intern Med. 130. 40-44 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kakoki M,Hirata Y,Hayakawa H,Nishimatsu H,Suzuki Y,Nagata D,Suzuki E,Kikuchi K,Nagano T,Omata M: "Effects of vasodilatory beta-adrenoceptor antagonists on endothelium-derived nitric oxide relcasc in rat kidncy" Hypertension. 33. 467-471 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kakoki M,Matsumoto A,Nagata D,Kobayakawa N,Kimura K,Momomura S,Omata M,Hirata Y: "Analysis of nitric oxide in the exhaled air of patients with chronic glomerulonephritis. Clin Nephr(in press)" Clin Nephr. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Nakatsubo N,Kojima H 他: "Dircct evidence of nitric oxide production from bovine aortic endothelial cells using new fluorescence indicators: diaminofluoresceins." FEBS Lett. 427. 263-266 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Kojima H,Nakatsubo N,他: "Detection and imaging of nitric oxide with novel fluorescent indicators: diaminofluoresceins." Anal Chem. 70. 2446-2453 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Matsumoto A,Hirata Y,他: "Increased excretion of nitric oxide in exhaled air of patients with chronic renal failure." Clin Sci. 96. 67-74 (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Matsumoto A,Momomura S,他: "The effect of nitric oxide on gas exchange in patients with congestive heart failure." Ann Intern Med. 130. 40-44 (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Kakoki M,Hirata Y,他: "Effects of vasodilatory β-adrenoceptor antagonists on endothelium-derived nitric oxide release in rat kidney." Hypertension. 33. 467-471 (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Kakoki M,Matsumoto A,他: "Analysis of nitric oxide in the exhaled air of patients with chronic glomerulonephritis." Clin Nephr. (in press).

    • Related Report
      1998 Annual Research Report
  • [Publications] Matsumoto A, Momomura S, 他: "Inhaled nitric oxide and exercise capacity in congestive heart failure." Lancet. 349・9057. 999-1000 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Hirata Y, Hayakawa H, 他: "Receptor subtype for vasopressin-induced release of nitric oxide from rat kidney." Hypertension. 29. 58-64 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Kojima H, Hirata Y, 他: "Development of fluorescent indicator for the bioimaging of nitric oxide." Biol Pharma Bull. 20. 1229-1232 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Kojima H, Hirata Y, 他: "Development of a fluorescent indicator for nitric oxide based on the fluorescein chromophore." Chem Pharma Bull. 44. 373-375 (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Bremer V, Hirata Y, 他: "Role of nitric oxide in rat nephrotoxic nephritis : Comparison between inducible and constitutive nitric oxide synthase." J Am Soc Nephrol. 8. 1712-1721 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Ikenouchi H, Hirata Y, 他: "Negative inotropic effect of adrenomedullin in isolated adult rabbit cardiac ventricular myocytes." Circulation. 95. 2318-2314 (1997)

    • Related Report
      1997 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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