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MECHANISM AND PATHOPHYSIOLOGICAL IMPORTANCE OF THE APOPTOSIS IN ATHEROSCLEROSIS-STUDY WITH LDL KNOCK-OUT MOUSE-

Research Project

Project/Area Number 09670707
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Circulatory organs internal medicine
Research InstitutionGIFU UNIVERSITY

Principal Investigator

TAKATSU Hisato  GIFU UNIV.SCHOOL OF MED.ASSIST.PROF., 医学部・附属病院, 講師 (20187975)

Co-Investigator(Kenkyū-buntansha) FUJIWARA Hisayoshi  GIFU UNIV.SCHOOL OF MED.PROFESSOR, 医学部, 教授 (80115930)
TAKEMURA Genzou  GIFU UNIV.SCHOOL OF MED.ASSISTANT., 医学部・附属病院, 助手 (40283311)
Project Period (FY) 1997 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 1998: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1997: ¥2,100,000 (Direct Cost: ¥2,100,000)
KeywordsAPOPTOSIS / LDL RECEPTOR KNOCK OUT MOUSE / ATHEROSCLEROSIS / LDLレセプターノックアウトマウス
Research Abstract

To investigate the occurrence of apoptosis in the atherosclerotic lesion, ascending aortas of the low density lipoprotein receptor knock out (LDL KG) mice were stained with TUNEL methods after the feeding with high cholesterol diet for 6 or 9 months. TUNEL positive cells were observed in the thickened intimas increasing in their number as the lesion progressed. In addition, electron microscopic study confirmed the occurrence of apoptotic change in the foam cells in the atherosclerotic lesions.
To investigate the expression of apoptosis-related proteins in this model, anti-ICE, anti-Bax and anti-Bcl-2 were used to stain the aortas of the LDL KG mice. Although the expression of apoptosis-accelerating proteins, ICE and Bax was unchanged with the disease progression, Bcl-2 was less strongly expressed in the advanced stage than in the earlier stage.
LDL KG mice were given both an accelerator of apoptosis, galic acid, and a inhibitor of apoptosis, ZVAD-fmk intraperitoneally for 8 weeks. However, these systemic administration of these drugs did not significantly increase or decrease the size of atheroma or the number of TUNEL positive cells in the lesions.
As a conclusion, the apoptosis, a programmed cell death acts in the formation of atherosclerosis in this model, and the decrease of the expression of apoptosis-inhibiting protein, Bcl-2 may control the cell death and contribute the progression of atheroma. However, systemic administration of apoptosis controlling agents failed to influence the size or cell components of atherosclerotic lesion.

Report

(3 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • Research Products

    (11 results)

All Other

All Publications (11 results)

  • [Publications] 国島 明久: "LDLノックアウトマウスの粥状動脈硬化病変におけるICE Baxについて" 動脈硬化. 25. 131 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 国島 明久: "粥状動脈硬化におけるアポトーシス及びその関連蛋白の発現" Japanese Circulation Journal. 62. 290 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Yukihiro Hayakawa: "Opoptosis and Overexpression of Bax and mRNA in sumoth" Arterioscler.Throml.Vasc Biol. 19(in press). (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] KUNISHIMA A et al.: "Expression of ICE and Bax in the atherosclerotic lesion of LDL receptor knockout mouse" DOMYAKUKOKA. (25). 131 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] KUNISHIMA A et al.: "A poptosis and the expression of apoptosis related protein in the atherosclerosis" Jap Circ J. (62). 290- (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] HAYAKAWA Y,et al.: "A poptosis and overexpression of Bax and mRNA in smooth muscle cells within intimal hyperplasia of human radial arteries." Arterioscler Thromb Vasc Biol. (in press). (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 国島 明久: "LDLレセプターノックアウトマウスの粥状動脈硬化病変におけるICE.Boxについて" 動脈硬化. 25. 131-131 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] 国島 明久: "粥状動脈硬化におけるアポトーシス及びその関連蛋白の発現" Japanese Circulation Journal. 62. 290-290 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Yukihiro Hayakawa: "Apoptosis and overexpression of Bax and suRNA in smooth muscle" Anterioscler Thromb Vasc Biol. 19 (in press). (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] 国島明久 他: "LDLレセプターノックアウトマウスの粥状動脈病変におけるICE、Bax Bcl-2の発現について" 動脈硬化. 25. 131 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 鷹津久登 他: "Cardiac sympathetic nerve function assere by [^<131>Z]-metaiods" J Nucl Cordial. 4. 33-41 (1997)

    • Related Report
      1997 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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