• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Coronary angiogenesis induced by VEGF gene: Comparison of angiogenic vessels and gene expression

Research Project

Project/Area Number 09670756
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Circulatory organs internal medicine
Research InstitutionTokai University

Principal Investigator

MORI Hidezo  Tokai University School of Medicine, Associate Professor, 医学部, 助教授 (90146598)

Co-Investigator(Kenkyū-buntansha) ABE Sumihisa  Tokai University School of Medicine, Assistant Professor, 医学部, 講師 (50159430)
ANDO Kiyoshi  Tokai University School of Medicine, Assistant Professor, 医学部, 講師 (70176014)
Project Period (FY) 1997 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 1999: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1998: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1997: ¥2,600,000 (Direct Cost: ¥2,600,000)
KeywordsVEGF / ASO / angiogenesis / synchrotron radiation / angiography / microsphere / adeno-virus / アデノヴィールス / アデノウイルス / マイクロスフェア-
Research Abstract

In this term of the project, we revealed that Lac-Z could be introduced into ischemic myocardial tissue by using adeno-virus vector, that VEGF165 containing adeno-virus relieved the ischemic myocardium and that new drug delivery system: gelatin hydrogel enhanced the efficiency of gene therapy without using adenovirus vector. In H-9, microvascular ischemia was created in 30 rabbits. One hour after the ischemia, gene delivery was performed. Four weeks later, changes in myocardial function and myocardial blood flow were compared with those in the acute pahse of ischemia. In H-10, we tested whether efficiency of gene (VEGF) delivery can be enhanced by the use of gelatin hydrogel in rabbit ASO model. In H-11, supplemental experiments were added and scientific manuscript was written.

Report

(4 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • 1997 Annual Research Report
  • Research Products

    (26 results)

All Other

All Publications (26 results)

  • [Publications] E, Tanaka et al: "Amelioration of microvascular myocardial ischemica by gene transfer of vascular endothelial growth factor in rabbits"J. Thorac. Cardior. Sur.. (印刷中). (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Y. Nakajima et al: "An analysis of interamyocardial vascular volume distribution studied by synchrotron radiation-excited x-ray fluorescence"Am. J. of Physiol. 277. H2353-H2362 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] K. Ito et al: "A microanglographic technique using synchrotron radiation to visualize dermal circulation in vivo"Plast Reconst Surg.. 102. 1128-1133 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] S. Takeshita et al: "Endo-thelium-dependent relaxation of collateral microvessels following intramuscular gene transfer of vascular endothelial growth factor"Circulation. 98. 1261-1263 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] S. Takeshita et al: "Microangiographic assessment of collaterial vessel formation following direct gene transfer of vascular endothelial growth factor in rats."Cardiovascular Research. 35. 547-552 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] S. Takeshita et al: "Use of synchrotron radiation microangiography to assese development of small collateral arteries in a rat model of hindlimb ischemia"Circulation. 95. 805-808 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Y. Tanaka et al: "Medical Applications of Syncrotron radiation"Springer-Verlag. 200 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] E. Tanaka, et al.: "Amelioration of microvascular myocardial ischemia by gene transfer of vascular endothelial growth factor in rabbits"J. Thorac. Cardiov. Sur.. (in press). (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Y. Nakajima, et al.: "An analysis of intramyocardial vascular volume distribution studied by synchrotron radiation-excited x-ray fluorescence"Am j.of physiol.. 277. H2353-H2362 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] K. Ito, et al.: "A microangriographic technique using synchrotron radiation to visualize dermal circulation in vivo."Plast Reconst. Surg.. 102. 1128-1133 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] S.Takeshita, et al.: "Endothelium-dependent. Relaxation of collateral microvessels following intramuscular gene transfer of vascular endothelial growth factor."Circulation. 98. 1261-1263 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] S.Takeshita, et al.: "Microangiographic assessment of collaterial vessel formation following direct gene transfer of vascular endothelial growth factor in rats"Cardiovascular Research. 35. 547-552 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] S.Takeshita, et al.: "Use of synchrotron radiation microangiography to assess development of small collateral arteries in a rat model of hindlimb ischemia."Circulation. 95. 805-808 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Y. Tanaka, et al.: "Medical Applications of Synchrotron Radiation"Springer-Verlag. 200 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] E.Tanaka.etal: "Amelioration of microvascular myocardial ischemia by gene transfer of vascular endothelial growth factor in rabbits"J.Thorac.Cardiov.Sur.. (印刷中). (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] Y.Nakajima.etal: "Ananalysis of intramyocardial vascular volume distribution studied by synchrotron radiatim-excited x-ray fluorescence"Am.J of Physiol. 277. H2353-H2362 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] A.Tanaka.etal: "Branching patterns of intramural coronary vessels determined by microangiography using syhchrotron radiation"Am.J of Physiol. 276. H2262-H2267 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] H.Mori.etal: "Synchrotron microaugingraphy reveals configurational changes and to-and-fro flow in intramyocardial vessels"Am.J of Physiol. 276. H429-H437 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] E.Tanaka.etal: "Digitized cerebral synchrotron radiation augiography:Quantitatine evaluation of the canine circle of willis and its langeand small branches"Am.J Neuroradiol. 20. 801-806 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] S.Takechita,et al: "Endothelium depenpent relaxation of cullaleral microvessels after inframural gene transfer" circulation. 98. 1261-1263 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] K.Ito,et al.: "A microangiographic cechnigve using synchrction rediation to visualizedermal circulution in vivo" Plastic and Reconstructive Surgery. 102. 1128-1134 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] H.Mori,et al: "Synchrotron microangiography roveals confifurational changes and to and fro flow in intromyocardial vessels." American Journal of Physiology. 276. H429-H437 (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Takeshita,S.et.al.: "Microangiographic assessment of colluteral vessel formation folloowing direct gene transfer of VEGF" Cardiovascular Research. 35. 547-552 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Takeshita,S.et.al.: "Use of SR Microangiography to assess development of snall collateral arteries in a rat model" Circulation. 95. 805-808 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 盛英三,他: "微小血管撮影" BME. 11. 29-36 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Y.Tanaka,H.Mori,et.al: "Haga 97'Proceedings" Springer Verlag (in press),

    • Related Report
      1997 Annual Research Report

URL: 

Published: 1997-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi