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A study on rapid diagnosis of septicemia.

Research Project

Project/Area Number 09670810
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Pediatrics
Research InstitutionYamaguchi University

Principal Investigator

TSUKAHARA Masato  School of Allied Health Sciences, Yamaguchi University, Professor, 医療技術短期大学部, 教授 (20136188)

Co-Investigator(Kenkyū-buntansha) IINO Hidechika  School of Allied Health Sciences, Yamaguchi University, Assistant Professor, 医療技術短期大学部, 助手 (20284276)
Project Period (FY) 1997 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 1998: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1997: ¥2,200,000 (Direct Cost: ¥2,200,000)
KeywordsSepticemia / Cat scratch disease / Bartonella henselae / PCR / 16SリボゾームDNA
Research Abstract

1. Polymerase chain reaction (PCR) method was developed to identify septicemia caused by staphylococcus, group B streptococcus, yersinia, mycoplasma and mycobacterium.
2. Serological method for identification of Bartonella henselae infection was developed.
1) A total of4l patients out of 119 patients with having suspicion of cat scratch disease were serologically diagnosed as cat scratch disease. Of these 41 patients, 14 had systemic Bartonella henselae infection including fever of unknown origin, granuloma of the liver and/or spleen.
2) The prevalence of antibodies against Bartonella henselae among Japanese was 6.4% (17/267).
3) The prevalence of antibodies against Barlonella henselae was 30% (6/20) in domestio cats, and 7.6% (4/52) in domestic dogs.
4) Bartonella henselae infections caused by domestic dogs were identified in two patients, suggesting that domestic dog as well as cat can serve as a potential reservoir of Bartonella henselae in man, and the owner of such dog might be at risk to have Bartonella henselae infections.
3. PCR method to detect Barionella henselae Bartonell was developed.
1) The prevalence of Bartonella henselae DNA in the peripheral blood in cats and dogs were 6.2% (1/16), and 5.7% (3/52), respectively.
2) The prevalence of Bartonella henselae DNA in the oral swabs in cats and dogs were 12.5% (2/16), and 55.5% (5/9), respectively.

Report

(3 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • Research Products

    (39 results)

All Other

All Publications (39 results)

  • [Publications] 塚原正人 他: "猫ひっかき病の血清診断" 医学と生物学. 134(4). 113-116 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 塚原正人 他: "猫ひっかき病" 化学療法の領域. 13(9). 1635-1638 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 藤井玲子 他: "受身赤血球凝集反応によるBartonella henselae抗体の検査" 医学と生物学. 135(5). 211-214 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 飯野英親 他: "アナムネーゼに“ペット飼育歴"が必要な時代" 看護実践の科学. 22(12). 82 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 常岡英弘 他: "間接蛍光抗体法によるBartonella henselae抗体価測定-B.henselae単独抗原とVero細胞共培養抗原との比較-" 感染症学雑誌. 72(8). 801-807 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 常岡英弘 他: "健常人の血清抗Bartonella henselae IgG抗体保有率" 感染症学雑誌. 73(1). 90-91 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Tsukahara M et al.: "Bartonella henselae infection from a dog" Lancet. 352(9141). 1682 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Sugio Y et al.: "Translocationt(X ; 21)(913.3 : P11.1)in a girl with Menkes disease" Am J Med Genet. 79. 191-194 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Tsukahara M et al: "New dominant syndrome of microcephaly, facial abnormalities, micromelia and-mental retardation" J Hum Genet. 43(4). 224-227 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Hagiwara K et al: "An epidemic of whooping cough-like illness due to chlamydia pneumonine in a junior high school" Padiatr Infect Dis J. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Matsuda M et al: "Familial isolated noncompaction of ventricular myocardium" J Hum Genet. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Tsukahara M et al: "Congenital heart defects in Sotos syndrome" Am J Med Genet. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Tsukahara M,Yamamoto K,Tsuneoka H,Uchida M: "Serological Diagnosis of cat scratch disease." Medicine and Biology. 134 (4). 113-116 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Fujii R,Fujisawa K,Yamamoto K,Tsuneoka H,Tsukahara M: "detection of antibodies to Bartonella henselae by the passive hemagglutination test." Medicine and Biology. 135 (5). 211-214 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Tsuneoka H,Fujii R,Yamamoto K, Fujisawa K,Iino H,Matsuda M,Tsukahara M: "determination of anti-Bartonella henselae antibody by indirect fluorescence antibody test-Comparison of two types of antigen : Non-cocultivated B.henselae and cocultivated B.henselae with Vero cells." J Jpn Assoc Infec Dis. 72. 801-807 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Tsukahara M,Tsuneoka H,Iino H,Ohno K,Murano I: "Bartonella henselae infection from a dog." Lancet. 352. 1682 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Sugio Y,Sugio Y,Kuwano A,Miyoshi O,Yamada K,Niikawa N,Tsukahara M: "Translocation t(X ; 21) (q13.3 ; p11.1) in a girl with Menkes disease." Am J Med Genet. 79. 191-194 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Tsukahara M,Sugio Y: "New dominant syndrome of microcephaly, facial abnormalities, micromelia and mental retardation." J Hum Genet. 43 (4). 224-227 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Tsuneoka H,Fujii R,Yamamoto K,Fujisawa K,Iino H,Tsukahara M: "Prevalence of serum IgG antibody against Bartonella henselae in an asymptomatic Japanese population." J Jpn Assoc Infec Dis. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Murano I,Yoshii H,Kurashige H,Sugio Y,Uchida M,Shinohara T,Tsuneoka H,Yamamoto Y,Iino I,Fujii R,Fujisawa K,Tsukahara M: "Three children with systemic cat scratch disease." J Jpn Assoc Infec Dis. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Hagiwara K,Ouchi K,Tashiro N,Azuma M,Tsukahara M: "An epidemic of whooping cough-like illness due to chlamydia pneumoniae in a junior high school." Pediatr Infec Dis J. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Matsuda M,Tsukahara M,Kondoh O,Mito H: "Familial isolated noncompaction of ventricular myocardium." J Hum Genet. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Tsukahara M,Murakami K,Iino H,Tateishi H,Fujita K,Uchida M: "Congenital Heart Defects in Sotos Syndrome." Am J Med Genet. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 塚原正人他: "猫ひっかき病の血清診断" 医学と生物学. 134(4). 113-116 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] 塚原正人他: "猫ひっかき病" 化学療法の領域. 13(9). 1635-1638 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] 藤井玲子他: "受身赤血球凝集反応によるBartonella henselae抗体の検査" 医学と生物学. 135(5). 211-214 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] 飯野英親他: "アナムネーゼに“ペット飼育歴"が必要な時代" 看護実践の科学. 22(12). 82 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] 常岡英弘他: "間接蛍光抗体法によるBartonella henselae抗体価測定-B.henselae単独抗原とVero細胞共培養抗原との比較-" 感染症学雑誌. 72(8). 801-807 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 常岡英弘他: "健常人の血清抗Bartonella henselae IgG抗体保有率" 感染症学雑誌. 73(1). 90-91 (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Tsukahara M etal.: "Bartonella henselae infection from a dog" Lancet. 352(9141). 1682 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Sugio Y etal.: "Translocation t(X;21)(913.3:P11.1) in agirl with Menkes disease" Am J Med Genet. 79. 191-194 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Tsukahara M.etal.: "New dominant syndrome of microcephaly,facial abnormalities,micromelia and mental retardation" J Hum Genet. 43(4). 224-227 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Hagiwara K.etal: "An epidemic of whooping cough-like illness dueto chlanydia pneumoniae in a:junior high school" Padiatr Intect Dis J. (in press).

    • Related Report
      1998 Annual Research Report
  • [Publications] Matsuda M etal: "Familial isolated noncompaction of ventricular myocardium" J Hum Genet. (in press).

    • Related Report
      1998 Annual Research Report
  • [Publications] Tsukahara M etal: "Congenital heart defects in Sotos Syndrome" Am J Med Genet. (in press).

    • Related Report
      1998 Annual Research Report
  • [Publications] Hagiwara K, 他: "An epldemic of whooping cough-like illness caused by chlarmydia pneumoniae in a junior high school" Pediatr.Inf.Dis. (発表予定).

    • Related Report
      1997 Annual Research Report
  • [Publications] 塚原正人, 他: "猫ひっかき病の血清診断" 医学と生物学. 134(4). 113-116 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 藤井玲子, 他: "受身赤血球凝集反応によるBartonella heuselae抗体の検査" 医学と生物学. 135(5). 211-214 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 塚原正人, 他: "猫ひっかき病" 化学療法の領域. 13(9). 1635-1638 (1997)

    • Related Report
      1997 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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