|Budget Amount *help
¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1998: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1997: ¥700,000 (Direct Cost: ¥700,000)
Object and methods :
Nine children with short stature were tested. I classified them in two groups according to results of growth hormone (OH) stimulation tests and nocturnal growth hormone secretion. Four belonged to growth hormone deficiency group (GND), male! female = 3/1, aged 4 to 10 years-old and 5 to normal short group (NS) . male/ female = 3/2, aged 5 to 13 years-old. To collect sample saliva, gave 2 g of cotton in the mouth for 30 minutes and centrifuged for 5 minutes at 1,500rpm. Saliva OH were measured using highly sensitive enzyme immuno assay (EIA). serum GH were measured with 2 antibody beads method.
Dilution test :
50mu1 of sample saliva was used for HA.Sensitivity of measurement system was 0.03pg/ tube. According the result detection limit of OH in saliva was O.6pg/ml.
Basal value of saliva OH was 5.2+/-4.5 pg/nd (mean+/-SD) in NS and 3.8+/-4.6pg/ml in GHD, respectively. Patterns of serum and saliva OH changed parallely. From these results, it considered that saliva OH concentration depended on serum GH concentration, and that evaluation of OH secretion ability was possible by OH measurement in saliva.
Good correlation between peak serum OH and peak saliva OH was observed( y=1.93x-3.31, r=0.84, p<0.001 , n= 22) . Then I studied IgG in saliva as standardization material better correlation between peak serum OH and peak saliva GH/IgG was observed( y=0.18x-0.24, r=0.86, p<0.001 , n= 17).