| Project/Area Number |
09670823
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Pediatrics
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| Research Institution | Sapporo Medical University |
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Principal Investigator |
KUDOH Tooru Sapporo Medical University, School of Medicine, Asso. Pro., 医学部, 助教授 (30117618)
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| Project Period (FY) |
1997 – 1999
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| Project Status |
Completed (Fiscal Year 1999)
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| Budget Amount *help |
¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 1999: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1998: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1997: ¥2,100,000 (Direct Cost: ¥2,100,000)
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| Keywords | Parvovirus / B19 |
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| Research Abstract |
The genetic diversity in the VP1 unique region, which is important for the neutralizing response to human parvovirus B19 was examined by the mismatch detection method using Non-isotopic RNase Cleavage Assay (NIRCAィイD1TMィエD1) and direct nucleotide sequencing. Twenty three B19 strains derived from various sources between 1986 and 1997 were examined. Three electrophoresis patterns were observed by NIRCAィイD1TMィエD1. The nucleotide sequence showed that there were 14 nucleotide changes and 4 amino acid substitutions in comparison with Au strains employed as a standard strain. The nucleotide variability of all samples ranged from 0.3% to 2.7% and the amino acid variability ranged from 1.0% to 3.0%. They were classified into three types according to NIRCAィイD1TMィエD1. Type 1 and 3 had similar sequences, but the type 2 sequence was quite different. Although there were some nucleotide variations in the same NIRCAィイD1TMィエD1 type, these were silent. However, there was no relationship between the clinical features and NIRCAィイD1TMィエD1 types or between clinical features and nucleotide sequence. All samples obtained before 1987 were NIRCAィイD1TMィエD1 type 2. On the other hand, 19 for 20 samples obtained after 1989 were NIRCAィイD1TMィエD1 type 1. The other sample obtained in 1992 was type 3. The results suggest that the B19 strain of type2 disappeared by 1988 and changed to other B19 strains such as type 1 and type 3 after 1988, indicating a correlation between genome type and prevalence. NIRCAィイD1TMィエD1 is a convenient method for screening mutations due to its simplicity and quickness.
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