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Study on cytological characteristics of myelodysplastic syndrome (MDS) in children.

Research Project

Project/Area Number 09670830
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Pediatrics
Research InstitutionDOKKYO UNIVERSITY SCHOOL OF MEDICINE

Principal Investigator

EGUCHI Mitsuoki  Dokkyo University School of Medicine, Medicine, Professor, 医学部, 教授 (60020799)

Project Period (FY) 1997 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 1998: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1997: ¥1,000,000 (Direct Cost: ¥1,000,000)
Keywordsmyelodysplastic syndrome / electron microscope / peroxidase / megakryoblasts / Down's syndrome / Fanconi's anemia / blast cell / apoptosis / MDS
Research Abstract

Erytliroblast, granulocyte and megakaryocyte series of cells from MDS in children and adults were studied by electron microscopic and cytochemical methods. Fourty three patients of child, adult and secondary cases of MDS were included in this study. Major abnormal finding of granulocytes was nuclear pocket, which was most frequently observed in the cells from patients with Down's syndrome (4 out of 7 cases). Other abnormal findings in granulocytes were deformation of nucleus, Auer body-like elongated granules, and Chediak-Higashi-like large granules. In erythroblasts, sponge-like deformation of nuclear chromatin was predominant in children. Polynudear and deformed nuclear erytliroblasts were also noted. Megakaryocyte and platelet changes, such as microinegakaryocytes and giant platelets, 'were not frequently observed. Refractory anemia revealed predominant erythroblastic changes, that is, iron-loaded mitochondda was observed in 5 out of 11 patients, and sponge-like change of nucleus in 4 out of 11 cases. RAEB-T showed most predominant granulocytic changes. Nuclear pockets were obseved in 8 out of 11 cases in this sub-type of MDS.Apoptosis was observed in erythroblast and granulocyte series of cells in all MDS sub-types.
Cytochemically, the number of myeloperoxidase-posilive granules were decreased in MIDS.Some of immature cells in MDS showed platelet peroxidase reaction, suggesting that the blasts had megakaryocyte characteristics. Electron microscopic and cytochemical methods were useful to find abnormalities in MDS cells which could not detect by lightmicroscopic techniques, and MiDS of children was not identical to that of adults.

Report

(3 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • Research Products

    (9 results)

All Other

All Publications (9 results)

  • [Publications] Komatsu, N: "GATA-1 and erythropoietin receptor genes are highly expressed in erythroleukemia." Exp.Hematol.26. 1148-1154 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 江口光興: "小児のMDSの細胞学的特性" 日本小児科学会雑誌. 103(5) in press. (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] N.Komatsu et al.: "GATA-1 and erythropoietin receptor genes are highly expressed in erythroleukemia." Exp.Hematol.26. 1148-1154 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] M.Eguchi: "Cytological characteristics of MDS in children. (in Japanese)" J.Jpn.Pediatr.Soc.103 (5) (in press.).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Komatsu,N: "GATA-1 and erythropoietin receptor genes anr highly expressed in erythroleukemia." Exp.Hematol.26. 1148-1154 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 江口光興: "小児のMDSの細胞学的特性" 日本小児科学会雑誌. in press.

    • Related Report
      1998 Annual Research Report
  • [Publications] 杉田憲一: "小児骨髄異形成症候群(MDS)の治療経験" 小児科診療. 60(8). 1371-1374 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Komatsu N.: "In vitro development of erythroid and megakaryocytic cells from UT-7 subline UT-7/GM." Blood. 89(11). 4021-4033 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 江口光興: "骨髄異形成症候群の項 今日の小児治療指針11版、矢田純一他編、分担執筆 p373" 医学書院, 688 (1997)

    • Related Report
      1997 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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