| Project/Area Number |
09670847
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Pediatrics
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| Research Institution | Fujita Health University |
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Principal Investigator |
SUGA Sadao Fujita Health University, School of Medicine, Lecturer, 医学部, 講師 (70257616)
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| Co-Investigator(Kenkyū-buntansha) |
YOSHIKAWA Tetsushi Fujita Health University, School of Medicine, Associate Professor, 医学部, 助教授 (80288472)
ASANO Yoshizo Fujita Health University, School of Medicine, Professor, 医学部, 教授 (40131180)
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| Project Period (FY) |
1997 – 1999
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| Project Status |
Completed (Fiscal Year 1999)
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| Budget Amount *help |
¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 1999: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1998: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1997: ¥1,400,000 (Direct Cost: ¥1,400,000)
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| Keywords | heat-labile enterotoxin / VZV / experimental model / mouse / humoral immunity / cellular immunity / 細胞性免疫 / アジュバント / 感染病理 |
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| Research Abstract |
The invasion of varicella-zoster virus (VZV), cellular and humoral immune responses to VZV were investigated in mouse model. A live varicella vaccine, Oka strain was administered to BALB/c or ICR mice with heat-labile enterotoxin mutant (LT mutant) prepared from Escherichia coli as an adjuvant via nasal route. Loss of body weight was observed in most mice after administration of the mixture of LT mutant and VZV one. VZV DNA was infrequently detected in brain tissues of a small number of the mice. One group of mice was immunized with VZV and mutant toxin 3 times in an interval of 2 months. Four weeks after last immunization, the mice showed positive reaction by the foot pad evaluation, suggesting the induction of cellular immune response to VZV. An another group of mice was immunized with the mixture one or 3 times in an interval of 2 weeks, 2 months and 6 months. Four weeks after last immunization, antibody activities to VZV were examined by the neutralization test. The antibody activities were not detected in the mice immunized one and 3 times in an interval of 2 weeks. However, those immunized 3 times in an interval of 2 months and 6 months had high antibody activities to VZV. These data suggest that LT mutant has strong adjuvant activity and may be useful for providing specific immunities to VZV and preventing development of herpes foster in future.
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