| Project/Area Number |
09670865
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Dermatology
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| Research Institution | Tohoku University |
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Principal Investigator |
TERUI Tadashi Tohoku University hospital Lecturer, 医学部・附属病院, 講師 (30172109)
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| Co-Investigator(Kenkyū-buntansha) |
TABATA Nobuko Tohoku University hospital, Research Associate, 医学部・附属病院, 助手 (00270835)
田中 美佐子 東北大学, 医学部, 助手 (90271907)
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| Project Period (FY) |
1997 – 1998
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| Project Status |
Completed (Fiscal Year 1998)
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| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 1998: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1997: ¥2,300,000 (Direct Cost: ¥2,300,000)
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| Keywords | complement / C3 / psoriasis / ultraviolet / UVB / UVA / PUVA / UVB erythemq |
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| Research Abstract |
The present study was designed to investigate by using ELISA and RT-PCR methods whether PUVA or UVB treatment affects C3 production byIFNgamma-stimulated keratinocytes cultured in serum-free medium. The results showed that PUVA and UVA reduced the C3 production by IFNgamma-stimulatedepidermal keratinocytes dose-dependently, although the effect of PUVA was stronger than that of UVA alone. Interestingly, UVB induced an enhancement of the C3 production at lower doses ranging from 10 to 50 mJ/cm^2, while it reduced the production at higher doses of 75 and 100 mJ/cm^2. This phenomenon was found at both the protein and mRNA level. In every experiment, changes in C3 mRNA levels preceded those of its protein levels. In our experimental system, PUVA, UVA, or UVB treatment did not affect theC3 production without IFNgamma-stimulation. The observations of our present study suggest that a reduction of the C3 production by PUVA or UVA treatment may explain in part why PUVA is effective in the treatment of inflammatory dermatoses such as psoriasis and other sterile pustular dermatoses, and the results of the UVB experiments may explain why there are both pro-inflammatory and anti-inflammatory effects.
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