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Analysis of Fibrillogenesis for Inactivation of TGF-beta by Decorin

Research Project

Project/Area Number 09670866
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Dermatology
Research InstitutionChiba University School of Medicine

Principal Investigator

SHINKAI Hiroshi  Chiba Univ., Sch.Med., Professor, 医学部, 教授 (90030957)

Co-Investigator(Kenkyū-buntansha) ENDO Hideji  Chiba Univ., Sch.Med., Assistant, 医学部, 助手 (50282489)
KURODA Kei  Chiba Univ., Sch.Med., Assistant, 医学部, 助手 (90225300)
HATAMOCHI Astushi  Chiba Univ., Sch.Med., Associato Professor, 医学部, 助教授 (90172923)
築藤 玲子  千葉大学, 医学部・附属病院, 講師 (70218048)
Project Period (FY) 1997 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 1998: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1997: ¥2,000,000 (Direct Cost: ¥2,000,000)
KeywordsTGF-beta / interleukin-4 / hypertrophic scar / systemic sclerosis ; / skin fibrosis. / デコリン / デルマトポンチン
Research Abstract

Dermatopont in is a recently discovered extracellular matrix protein with proteoglycan and cell binding properties and is assumed to play important roles in cell-matrix interactions and matrix assembly. En this study we examined the expression of dermatopont in mRNA and protein in skin fibroblast cultures from patients with hypertrophic scar (HS) and patients with systemic sclerosis (SSc). Dermatopont in mRNA and protein levels were reduced in fibroblast cultures from HS lesional skin compared to fibroblasts from normal skin of the same HS patient. Fibroblast cultures from SSc patient involved skin also showed significant reduction of dermatopontin expression compared to normal skin fibroblasts from healthy individuals. We investigated further the effects of cytokines and matrix collagen on dermatopontin expression in normal cultured fibroblasts. Transforming growth factor-beta1 increased dermatopontin mRNA and protein levels, while interleukin-4 reduced dermatopontin expression. Substrate coated with type I collagen reduced dermatopontin mRNA levels, the reduction being more prominent in three-dimensional collagen matrices. Our results suggest that the decreased expression of dermatopontin is associated with the parhogenesis of fibrosis in HS and SSc, and that the effect of the cytokines and matrix collagen on dermatopont in may have important implications for tissue remodeling or fibrosis.

Report

(3 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • Research Products

    (22 results)

All Other

All Publications (22 results)

  • [Publications] Tsukifuji R. Sakai Y. Hatamochi A. Shinkai H: "Geno expression of matrixmetallproteinasel (interstitial collagenase) and-3 (stromelysin-1) in basal cell carcinoma by in situ hybrization using chondroition ABC lyase." Histochem J. 29. 401-407 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kuroda, K.and Shinkai, H.: "Differential regulations of matrix metalloproteinases and tissue inhibitors of metalloproteinases in dermal fibroblasts by dibutyril cyclic AMP" J.Dermatol. Sci.,. 15. 95-100 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kuroda, K.and Shinkai, H.: "Downregulation of decorin expression in dermal fibroblasts by interleukin-4" Arch. Dermatol. Res. 289. 476-480 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kuroda, K.and Shinkai, H.: "Decorin and glycosaminoglycan synthesis in skin fibroblasts from patients with systemic sclerosis," Arch. Dermatol. Res. 289. 481-485 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] K.Kuroda, H.Shinkai: "Gene expression of type I and III collagen, decrin, matrix metalloproteases and tissue in-hibitors of metalloproteases in skin fibroblasts from patients with systemic sclerosis" Arch. Dermatol. Res. 289. 567-572 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kuroda. K., Tsukifuji. R., Shinkai. H: "Increased expression of heat-shock protein 47 is associated with overproduction of type I procollagen in systemic selerosis skin fibroblasts" J.Invest. Dermatol.111. 1023-1028 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Tsukifuji R,Sakai Y,Hatamochi, A,Shinkai H.: "Gene expression of matrix metall proteinase l (interstitial collagenase) and-3 (stromelysin-1) in basal cell carcinoma by in situ hybridization using chondroitin ABC lyase." Histochem J.29. 401-407 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kuroda, K.and Shinkai, H.: "Differential regulations of matri7 metalloproteinases and tissue inhibitors of metalloproteinases in dermal fibroblasts by dibutyril cyclic AMP." J.Dermatol.Sci.15. 95-100 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kuroda, K.and Shinkai, H.: "Downregulation of decorin expression in dermal fibroblasts by interleukin-4" Arch, Dermatol.Res.289. 475-480 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kuroda, K.and Shinkai, H.: "Decorin and glycosaminoglycan synthesis in skin fibrpblasts from patients with systemic sclerosis" Arch, Dermatol.Res.289. 481-485 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] K.Kuroda, H.Shinkai: "Gene expression of type I and III collagen, decorin, matrix metalloproteases and tissue inhibitors of metalloproteases in skin fibroblasts from patients with systemic sclerosis" Arch, Dermatol.Res.289. 567-572 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kuroda, K., Tsukifuji, R., Shinkai, H: "Increased expression of heat-shock protein 47 is associated with overproduction of type I procollagen in systemic sclerosis skin fibroblasts" J.Invest.Dermatol.111. 1023-1108 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Hatamochi A,Kuroda K,Shinkai H,Kohma H,Oishi Y,Inoue S.: "Regulation of matrix metalloproteinase (MMP) expression in cutis laxa fibroblasts : upregulation of MMP-1, MMP-3 and MMP-9 genes but not of the MMP-2 gene." Br.J.Dermatol.138. 757-762 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Tsukifuji R.Sakai Y,Hatamochi,A.Shinkai H: "Gene expression of matrixmetallproteinasel(intersitial collagenase)and -3(stromelysin-1)in basal cell carcinoma by in situ hybridization using chondroitin ABC lyase." Histochem J. 29. 401-407 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] Kuroda,K.and Shinkai,H.: "Differential regulations of matrix metalloproteinases and tissue inhibitors of metalloproteinases in dermal fibroblasts by dibutyril cyclic AMP" J.Dermatol.Sci.15. 95-100 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] Kuroda,K and Shinkai,H.: "Downregulation of decorin expression in dermal fibroblasts by interleukin-4" Arch.Dermatol.Res. 289. 476-480 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] Kuroda K.and Shinkai,H.: "Decorin and glycosaminoglycan synthesis in skin fibroblasts from patients with systemic sclerosis" Arch,Dermatol.Res. 289. 481-485 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] K.Kuroda,H.Shinkai: "Gene expression of type I and III collagen,decorin,matrix metalloproteases and tissue in-hibitors of metalloproteases in skin fibroblasts from patients with svstemic sclerosis" Arch,Dermatol.Res. 289. 567-572 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] Kuroda,K.,Tsukifuji,R.,Shinkai,H: "Increased expression of heat-shock protein 47 is associated with overproduction of type I procollagen in systemic sclerosis skin fibroblasts" J.Invest.Dermatol.111. 1023-1028 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Kuroda,K., Shinkai,H.: "Gene expression of type I and III collagen,decorin,matrix metalloproteinases and tissue inhibitors of metalloproteinases in skin fibroblasts from patients with systemic sclerosis" Arch.Dermatol.Res.289. 567-572 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Kuroda,K., Shinkai,H.: "Downregulation of decorin expression in dermal fibroblasts by interleukin- 4" Arch.Dermatol.Res.289. 476-478 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Kuroda,K., Shinkai,H.: "Decorin and glycosaminoglycan synthesis in skin fibroblasts from patients with systemic sclerosis" Arch.Dermatol.Res.289. 481-485 (1997)

    • Related Report
      1997 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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