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Physiological and pathological analyses of prostagland in receptor knockout mouse skin

Research Project

Project/Area Number 09670876
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Dermatology
Research InstitutionKYOTO UNIVERSITY

Principal Investigator

IKAI Kouichi  Kyoto University Graduate School of Medicine, Associate Profe, 医学研究科, 助教授 (00135568)

Co-Investigator(Kenkyū-buntansha) NISHIGORI Chikako  Kyoto University Graduate School of Medicine, Instructor, 医学研究科, 助手 (50198454)
Project Period (FY) 1996 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 1998: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1997: ¥2,100,000 (Direct Cost: ¥2,100,000)
KeywordsProstanoids / Prostanoid Receptor / Knockout mice / Ultraviolet / Contact Dermatitis / Wound healing / 創傷治療
Research Abstract

The prostaglandins (PGs) and thromboxanes (TXs) produced are then released outside the cells by various stimuli and bind individual receptor systems that are coupled to the G-protein system. Eight kinds of receptors for prostanoids such as PGE (including isoforms ; EP1, EP2, EP3 and EP4), PGD, PGF, PGI and TX, have been demonstrated. Recently, knockout mice lacking prostanoid receptors have also been developed. We characterized the skin of PG receptor knockout mice. Macroscopically, we observed slight abnormal hair growth in PGE receptor knockout mice, but did not find any histological difference in the skin between knockout and control mice. Moreover, we did not find any difference in the expression of erythema and swelling and the number of sunburn cells after UVB irradiation to the ears between knockout and control mice. There were also no significant difference in the expression of primary or allergic contract dermatitis induced by PMA or DNCB.Wound healing after experimental ulcer formation in the skin is slightly delayed in PGE and TX receptors knockout mice. These results suggest that even if one PG receptor is deficient, other PG receptors can compensate the function of lacked receptor and totally no serious defects are expressed in knockout mice skin. Abnormal hair growth in PGE receptor knockout mice are under investigation.

Report

(3 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • Research Products

    (30 results)

All Other

All Publications (30 results)

  • [Publications] Okano-Mitani, H: "Human melanoma cells generate Leukofriene B_4 and C4 from leuko friene A_4" Arch Dermatol Res. 289・6. 331-336 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Ikai, K: "Serum level of major basic protein indicates disease activity in a topic dermafitis" J Eur Acad Dermatol. 9・1. 54-56 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Ikai, K: "Induction of apoptosis, P53 and heme oxygenase (32KD heat shock proteln) by a cytotoxic prostag landin, 12-PGJ2 in transformed endothelial cells" pros Leuko Ess Fatty Acid. 58(4). 295-300 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Sawai, T: "Cyclic adenosine monophosphate phosphodiestarase activity in peripheral blood mononuclear leukocytes from pationts with atopic dermatitis : correlation with respiratory atopy" Brit J Dermatol. 138(5). 846-848 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Nishigori, C: "Hind III liposomes suppress delayed-type hypersensitivity responses in vivo and production of epidermal IL-10 in vitro" J Immunol. 161(6). 2684-2691 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Ikai, K: "Psoriasis and the arachidonic a cid cascade" J Dermatol Sci. in press. (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Ikai, K: "Biochemical modulation of skin reactions in dermal and transdermal delivery of drugs" CRC press in press, (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 錦織 千佳子: "光線過敏症診断治療マニュアル" 全日本病院出版会, 9-18 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Morita K,Nishigori C,Sasaki MS,Matsuyoshi N,Ohta K,Okamoto H,Ikai K,and Imamura S: "Werner's syndrome-chromosome analysis of cultured fibroblasts and mitogen-stimulated lymphocytes" Brit J Dermatol. 136 (4). 620-623 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Okano-Mitani H,Ikai K and Imamura S: "Human melanoma cells generate leukotriene B4 and C4 from leukotriene A4." Arch Dermatol Res. 289 (6). 331-336 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Ikai K,Nakajima N,Ozaki M,Furukawa I,Aoshima T,Mitani T,Sawami M,Sayama S,Omoto M,Uehara M and Imamura S: "Serum level of major basic protein indicates disease activity in atopic dermatitis." J Euro Acad Dermatol. 9 (1). 54-56 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Ikai K,Kudo H,Toda K and Fukushima M: "Induction of apoptosis, p53 and heme oxygenase-1 by a cytotoxic prostaglandin, DELTA_<12->PGJ_2, in transformed endothelial cells." Pros Leuko Ess Fatty Acid. 58 (4). 295-300 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Sawai T,Ikai K and Uehara M: "Cyclic adenosine monophosphate phosphodiesterase activity in peripheral blood mononuclear leukocytes from patients with atopic dermatitis : Correlation to respiratory atopy." Brit J Dermatol. 138 (5). 846-848 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Nishigori C,Yarosh D,O'Connor A,Shreedhar VK,Ullrich S,Cox P and Kripke ML: "HindIII liposomes suppress delayed-type hypersensitivity responses in vivo and induce epidermal IL-10 in vitro." J Immunol. 161 (6). 2684-2691 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Matsumura Y,Nishigori C,Yagi T,Imamura S and Takebe H: "Mutations of p16 and p15 tumor suppressor genes and replication errors contribute independently to the pathogenesis of sporadic malignant melanoma." Arch Dermatol Res. 290 (4). 175-180 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Matsumura Y,Nishigori C,Yagi T,Imamura S and Takebe H: "Characterization of molecular defects in xeroderma pigmentosum group F in relation to its clinically mild symptoms." Hum Mol Genet. 7 (6). 969-974 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Yagi T,Matsumura Y,Sato M,Nishigori C,Mori T,Sijbers AM and Takebe H: "Complete restoration of normal DNA repair characteristics in group F xeroderma pigmentosum cells by over-expression of transfected XPF cDNA." Carcinogenesis. 19 (1). 55-60 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Ikai K: "Psoriasis and the arachidonic acid cascade." J Dermatol Sci. 20 (in press). (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Ikai,K: "Induction of apoptosis,p53 and heme oxygenase(32KD heat shock protein)by a cytotoxic prostaglandin,12-PGJ2 in transformed endothelial cells" Pros Leuko Ess Fatty Acid. 58(4). 295-300 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Sawai,T: "Cyclic adenosine monophosphate phosphodiestarase activity in peripheral blood mononucleat leukocytes from pationts with atopic dermatitis:correlation with respiratory atopy" Brit J Dermatol. 138(5). 846-848 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Yagi,T.: "Complete restoration of normal DNA repair characteristics in Group F xeroderma pigmentosum cells by over-expression of transfected XPF cDNA" Carcinogenesis. 19(1). 55-60 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Matsumura,Y.: "Characterization of molecular defects in xeroderma pigmentosum group F in relation to its clinically mild symptoms" Hum Mol Genet. 7(6). 969-974 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Nishigori,C.: "Hind III liposomes suppress delayed-type hypersensitivity responses in vivo and production of epidermal IL-10 in vitro" J Immunol. 161(6). 2684-2691 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Ikai,K: "Psoriasis and the arachidonic acid cascade" J Dermatol Sci. (in press). (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Ikai,K: "Biochemical modulation of skin reactions in dermal and transdermal delivery of drugs" CRC press (in press), (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Morita,K: "Werner's syndeone-chromosome analysis of cultured f-broblasts and mitogen-stimulated lymphocytes" Brit J Dermafol. 136・4. 620-623 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] OKano-Mitani,H: "Human melanoma cells generate Leukotriene B_4 and C_4 from leuko friene A_4" Arch Dermatol Res. 289・6. 331-336 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Ikai,K: "Serumlevel of majon basic protein indicates disease activity in atopic dermatitis" J Eur Acad Dermatol. 9・1. 54-56 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Ikai,k: "Induction of apoptosis,P^<53> and heme oxyginase (32 kD heatshck protein) by a cytofoxic prostaglandin,Δ^<12>-PGJ_2 in transfoued endothelial cells" Pros Leuko Ess Fatty Acid. (印刷中). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Sawai,T: "Cyclic adenosine mono phosphate phosphodiesterase activity in periphnol blood mononudear leukoajtes from patoents with atopic dermafifis" Brint J Dermatol. (印刷中). (1998)

    • Related Report
      1997 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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