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Differential effects of dominant negative mutants of desmoglein, desmocollin, and E-cadherin on cell-cell adhesion of keratinocytes.

Research Project

Project/Area Number 09670883
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Dermatology
Research InstitutionEhime University

Principal Investigator

SAYAMA Koji  EHIME UNIVERSITY,UNIVERSITYHOSPITAL,ASSISTANT PROFESSOR, 医学部附属病院, 講師 (80187286)

Project Period (FY) 1997 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 1998: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1997: ¥2,800,000 (Direct Cost: ¥2,800,000)
KeywordsKeratinocytes / Adenovirus Vector / Cre / loxP / Desmoglein-3 / Desmocollin-3 / Cadherin / Desmosome / Adherens junction / アデノウィルスベクター / アデノウイルスヘクター / クラチノカイト
Research Abstract

Epithelial cell-cell adhesion is considered to be important in development and maintenance of tissue integrity of stratified squamous epithelium. The purpose of this study is to investigate the roles of classic and desmosomal cadherins in the maintenance of keratinocyte cell-cell adhesion. With adenovirus vector (Ad), we studied the effect of mutants of E-cadherin, desmoglein 3 , desmocollin3 which were constructed by deleting a large part of the extracellular domain (E-cadDELTA EC,Dsg3DELTA EC, Dsc3DELTA EC) on HaCaT cell. First, we tried to make Ad that directly express the mutant. But due to the toxicity or other unknown mechanism, we could not make Ad. Then we used Ad Cre-loxP system. LoxP flanked mutant vector does not express itself, but co-infection of AdCre switches on the mutant transcription. Under high calcium condition, Dsg3DELTAEC expression disrupted only desmosome, Dsc3DELTA ECor E-cadDELTAEC expression disrupted desmosome and also adherens junction in inimunofluorescence staining. By 2h after calcium elevation, all the mutants inhibited desmosome formation. But only Dsg3DELTAEc could not inhibit adherens junction formation. These results suggest that desmoglein and desniocollin may play a different role in cell-cell adhesion.

Report

(3 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • Research Products

    (3 results)

All Other

All Publications (3 results)

  • [Publications] Yasushi Hanakawa et al.: "Differential effects of dominant negative mutants of desmoglein,desmocollin,and E-cadherin on cell-cell adhesion of keratinocytes." Journal of Investigative Dermatology(abst). (in press). (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Yasushi Hanakawa, Masayuki AmagaiYuji Shirakata, Koji Sayama and Koji Hashimoto: "Differential effects of dominant negative mutants of desmoglein, desmocollin, and E-cadherin on cell-cell adhesion of keratinocytes." Journal of Investigative Dermatology. (in press), abst. (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Yasushi Hanakawa: "Differential effects of dominant negative mutants of desmoglein, desmocollin, and E-cadherin on cell-cell adhesion of keratinocytes." Journal fo invetigative Dermatology,abst,. (in press). (1999)

    • Related Report
      1998 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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