The Structure and Function of the 450-kDa Autoantigen Expressed in Keratinocytes
| Project/Area Number |
09670889
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Dermatology
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| Research Institution | Oita Medical University |
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Principal Investigator |
FUJIWARA Sakuhei Oita Medical University, School of Medicine, Associate Professor, 医学部, 助教授 (90181411)
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| Co-Investigator(Kenkyū-buntansha) |
OKAMOTO Osamu Oita Medical University, School of Medicine, Assistant, 医学部, 助手 (40284799)
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| Project Period (FY) |
1997 – 1998
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| Project Status |
Completed (Fiscal Year 1998)
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| Budget Amount *help |
¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 1998: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1997: ¥3,100,000 (Direct Cost: ¥3,100,000)
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| Keywords | Bullous Pemphigoid / plectin / hemidesmosome / basal lamina / keratinocyte / intermediate filament / cell attachment / ヘミデスモゾーム / 自己抗体 / X クロモソーム |
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| Research Abstract |
We previously reported an individual with subepidermal blistering disease that resembled bullous pemphigoid both clinically and pathologically. Immunoblot analysis revealed that the patient's serum did not react with the 230- or 180-kDa bullous pemphigoid antigens, but recognized exclusively a 450-kDa epidermal polypeptide. To elucidate the molecular structure of this antigen, we have cloned and characterized the overlapping cDNAs. About 13.8kb cDNAs, covering 90% of total length, were isolated. It has homology with plectin and it was named plectin 2. Using the cDNAs as probes, human genomic library was screened and four overlapping clones were isolated. At 3' end of 10kb in the gene, no intron was found. Combined with the information from the cDNAs and the gene, four highly homologous repeats, composed of 534 amino acids, located at the carboxy terminal end of this molecule. As a result, plectin 2 is the molecule derived from the unique gene which differ from that encodes pectin.
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Report
(3 results)
Research Products
(8 results)
