| Project/Area Number |
09670897
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Dermatology
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| Research Institution | Juntendo University School of Medicine |
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Principal Investigator |
TSUBOI Ryoji Department of Dermatology, Juntendo University School of Medicine Associate Professor, 医学部, 助教授 (70221421)
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| Project Period (FY) |
1997 – 1998
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| Project Status |
Completed (Fiscal Year 1998)
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| Budget Amount *help |
¥2,900,000 (Direct Cost: ¥2,900,000)
Fiscal Year 1998: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 1997: ¥1,600,000 (Direct Cost: ¥1,600,000)
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| Keywords | Wound healing / MRSA / Animal model / Diabetes / Skin ulcer / purified sucrose-povidoneiodine / wound healing |
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| Research Abstract |
It is clinically well known that bacterial infection delays the healing of chronic skin ulcers. In this study, we succeeded in demonstrating the utility of hereditary diabetic mice as a model for observing the healing process in infectious wounds. infectious chronic cutaneous wounds were prepared by applying MRSA (methicillin-resistant Staphylococcus aureus) to two full-thickness wounds created in the dorsal skin of female G57BLKsJ db/db hereditary diabetic mice. MRSA was applied topically, and the wounds then covered by Cathereep film. Thirteen days after the application, the wounds were histologically evaluated by measuring re-epithelialization (%), area of granulation tissue (mm^2), capillary numbers and bacterial count. Results indicated that the diabetic mice had a high incidence of infectious ulcers and impaired healing compared with normal mice.
The following new findings were additionally made : (1) the covered wounds persisted longer and showed more severe manifestations than t
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he open wounds ; (2) a three-mm diameter wound was determined to be the appropriate experimental size for infectious wounds as larger wounds did not close after a one-month incubation period ; (3) 1 *10^6 was determined to be the optimum inoculum size ; (4) an average of four days are required to establish a MRSA infection ; (5) the MRSA-infected wounds remained unhealed after 13 days incubation ; (6) the methicillin-sensitive (MS)SA N315P and MRSA N315PZR strains had a similar pathogenicity with respect to the formation of infectious wounds in diabetic mice. When the mice with 3-mm size wounds, infected with 1 * 10^5 QS MRSA for 4 days, were treated with purified sucrose-povidone iodine every other day, their wounds closed earlier and had a lower bacterial count compared with wounds of the MRSA-infected and non-treated mice. Thus these results indicate that an infectious chronic ulcer prepared in the skin of diabetic mice is a good model for monitoring the healing process of infectious wounds and for evaluating the efficacy of agents. Less
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