| Project/Area Number |
09670899
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Dermatology
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| Research Institution | St.Marianna Medical University |
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Principal Investigator |
HIGAKI Megumu St.Marianna Medical University Institute of Mediucal Science, Associate Professor, 難病治療研究センター, 助教授 (70156553)
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| Co-Investigator(Kenkyū-buntansha) |
MIZUSHIMA Yutaka St.Marianna Medical University Institute of Mediucal Science, Professor, 難病治療研究センター, 教授 (40010409)
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| Project Period (FY) |
1997 – 1998
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| Project Status |
Completed (Fiscal Year 1998)
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| Budget Amount *help |
¥2,600,000 (Direct Cost: ¥2,600,000)
Fiscal Year 1998: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1997: ¥1,400,000 (Direct Cost: ¥1,400,000)
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| Keywords | Psoriasis vulgaris / apaptosis / vitamine D3 / keratinocytes / bcl-x / proliferation / Immunohistochemistry / RT-PCR / 尋常性乾癖 |
|---|
| Research Abstract |
Psoriasis is a chronic skin disease characterized by epidermal hyperproliferation in addition to inflammatory cell infiltration. Hyperproliferation in psoriasis is regulated by various mediators including growth factors and/or apoptotic mechanism. In psoriatic lesional skin, innumerable apoptotic cells has been shown by TUNEL(TdT-mediated dUTP nick end labeling) method. However, the factors which regulate apoptosis of keratinocytes are unknown. In this study, we analyzed the expression of Bcl-x in normal and psoriatic skin immunohistochernically, as Bcl-x, one of the proteins in Bcl-2 family, is known to prevent apoptosis. Furthermore, to clarify the action mechanisms of vitamine D3, we compared the expression of Bcl and DNA fragmanetation in psoriatic lesion aftertopical application of vitamin D3 to those before the treatment.
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