RADIOSENSITIVITY AND APOPTOSIS OF SPERMATOGONIA
| Project/Area Number |
09670917
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Radiation science
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| Research Institution | GUNMA UNIVERSITY |
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Principal Investigator |
HASEGAWA Masatoshi GUNMA UNIVERSITY SCHOOL OF MEDICINE,DEPARTMENT OF RADIOLOGY AND RADIATION ONCOLOGY,ASSISTANT PROFESSOR, 医学部, 講師 (50251111)
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| Co-Investigator(Kenkyū-buntansha) |
HAYAKAWA Kayoko GUNMA UNIVERSITY SCHOOL OF MEDICINE,DEPARTMENT OF RADIOLOGY AND RADIATION ONCOLO, 医学部, 教務員 (60182523)
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| Project Period (FY) |
1997 – 1998
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| Project Status |
Completed (Fiscal Year 1998)
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| Budget Amount *help |
¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1998: ¥500,000 (Direct Cost: ¥500,000)
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| Keywords | apoptosis / spermatogonia / radiosensitivity / p53 / knockout mice / TUNEL / 精粗細胞 / p53 |
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| Research Abstract |
The purpose of this study is to identify the mode of radiation-induced cell death in the tesitis and other tissue.
Male C57BL or BALB/cA Jcl-nu mice were irradiated with single doses of 0.5, 2.5 or 5.0 Gy to investigate the morphological features of radiation-induced cell death. Male p53 knockout mice, homozygously null p53(-/-) and heterozygous p53(+I-) mice (CBA/OYC), and wild type mice (CBA/OYC) were irradiated with a single dose of 5.0 Gy to determine whether the radiation- induced cell death was p53-dependent.
The radiation-induced cell death of spermatogonia reached a peak at 12 hours after irradiation, but that of other tissue was at 6 hours after irradiation. Nuclei of many abnormal spermatogonia were condensed and TUNEL-positive, though margination of nuclear chromatin and nuclear fragmentation was rare. The incidence of radiation-induced cell death in testis was markedly increased in p53(+I+) mice, but there was little change in p53(-/-) mice. Apoptosis of lymphocytes and fibrosarcoma cells inceased markedly following irradiation in the p53(+/+) or p53(+/-) genotype, but that increased just a little in the p53(-/-) genotype.
Spermatogonial cell death induced by irradiation is compatible with p53-dependent apoptosis, though the morphological features are not always typical.
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Report
(3 results)
Research Products
(20 results)
