| Project/Area Number |
09670949
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Radiation science
|
|---|
| Research Institution | Nara Medical University |
|---|
Principal Investigator |
YOSHIMURA Hitoshi Nara Medical University, Associate Professor, 医学部, 助教授 (60167012)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
MATSUO Yoshihiro Nara Medical University, Assistant, 医学部, 助手 (10244716)
IWATA Kazuro Nara Medical University, Assistant Professor, 医学部, 講師 (00201343)
OHNISHI Takeo Nara Medical University, Professor, 医学部, 教授 (60094554)
|
|---|
| Project Period (FY) |
1997 – 1998
|
| Project Status |
Completed (Fiscal Year 1998)
|
| Budget Amount *help |
¥2,700,000 (Direct Cost: ¥2,700,000)
Fiscal Year 1998: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1997: ¥1,500,000 (Direct Cost: ¥1,500,000)
|
|---|
| Keywords | Esophageal Cancer / Radiotherapy / Therapeutic Effect / Prognosis / Predictor / Cancer-related Gene / Apoptpsis / P53 |
|---|
| Research Abstract |
PURPOSE : To establish a biological predictor of the radiotherapeutic effects in patients with esophageal cancer, accumulations of p53 and apoptosis-related gene products in tumor tissues were examined for possible correlation with primary radiotherapeutic effects and prognosis.
MATERIALS AND METHODS : Thirteen patients with inoperable esophageal cancer were treated by radiotherapy alone from May 1997 to December 1998, using high energy photon (IOMVX) at total dose of 60-70 Gy(mean, 67Gy). Endoscopic biopsy specimens were obtained from tumor tissues prior to initiation of radiotherapy, after irradiation at a dose of 1OGy, and within a month after completion of radiotherapy. Biopsy specimens were fixed in formalin, and embedded in paraffin. Intracellular accumulation of p53, WAF1, Bax or Bcl-2 gene products was immunohistochemically assessed by ABC technique using antibodies against respective protains.
RESULTS : 1) The primary radiotherapeutic effects of 13 patients were rated as CR in 3, as PR in 9 and as NC in 1. Response rate was 92.3%. Three patients died due to relapse 5-8 months(mean, 6.3 months) after radiotherapy. Mean survival time was 12.5 months (ranging 5- 21 months) at the time of reporting. 2) Patients showing lower accumulation of either p53 or WAF1 in tumor lesions before radiotherapy tended to obtain CR with a higher incidence. 3) Patients showing lower accumulation of either p53 in tumor tissues before radiotherapy tended to be related with a more favorable prognosis. 4) Patients who showed increased accumulation of either p53 or WAFi in tumor tissues after radiotherapy tended to obtain CR with a higher incidence and were related with a more favorable prognosis.
CONCLUSION : Genetic status of p53 and WAF1 induction by irradiation for esophageal cancer cells were suggested to be good predictors for the radiotherapeutic effects. However, further accumulation of cases and follow-up study are necessary to evaluate these data.
|
