Identification of altered gene expression in psychotomimetic-treated animals and its changes in postmortem brains from patients with schizophrenia
| Project/Area Number |
09670989
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Psychiatric science
|
|---|
| Research Institution | Kobe University |
|---|
Principal Investigator |
SHIRAKAWA Osamu Kobe University School of Medicine Department of Psychiatry Assistant Professor, 医学部・附属病院, 講師 (40243307)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
HASHIMOTO Takeshi Kobe University School of Medicine Department of Psychiatry Instructor, 医学部・附属病院, 助手 (60294229)
|
|---|
| Project Period (FY) |
1997 – 1998
|
| Project Status |
Completed (Fiscal Year 1998)
|
| Budget Amount *help |
¥2,800,000 (Direct Cost: ¥2,800,000)
Fiscal Year 1998: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1997: ¥2,100,000 (Direct Cost: ¥2,100,000)
|
|---|
| Keywords | schizophrenia / phencyclidine / nRNA / phospholipase C / human brain / differential display / PCR |
|---|
| Research Abstract |
1. We searched for phencylidine-induced genes using mRNA differential disp1ay methods. One cDNA was indentified arid cloned in the nucleus accumbens of treated rats and the DNA sequence was analyzed in further details. This clone showed strong sequence homology to a human expressed sequence tag clone. We confirmed higher level of this mRNA in the nucleus accumbens at 24 hrs after phencylidine (8 mg/kg, s.c.) injection by nothern blot analysis. To identify the complete sequence of this mRNA, cDNA cloning is going on.
2. We immunoquantified PLC beta1, gamma1 and delta1 in the left prefrontal cortex and superior temporal cortex, nucleus accumbens and amygdala, and in the right superior temporal cortex of postmortem brains obtained from a total of 19 patients with schizophrenia and a total of 28 controls. PLC beta1 immunoreactivities were increased in the prefrontal cortex, while they were decreased in the left superior temporal cortex, as compared with the values in controls. There was no difference in PLC beta1 immunoreactivities in the nucleus accumbens, the amygdala or the right superior temporal cortex between schizophrenic patients and controls. PLC gamma1 and delta1 immunoreactivities did not differ between the two groups in any of the regions studied.
|
Report
(3 results)
Research Products
(15 results)
