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Analysis of the protein carboxyl methyltransferase gene as a brain specific repair enzyme

Research Project

Project/Area Number 09670992
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Psychiatric science
Research InstitutionEhime University

Principal Investigator

MIZOBUCHI Mutsuhiko  Ehime University, assistant professor, 医学部 附属病院, 講師 (00166042)

Co-Investigator(Kenkyū-buntansha) TAKEDA Ryohei  Ehime University, lecturer, 医学部, 助手 (60271087)
Project Period (FY) 1997 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 1998: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1997: ¥1,700,000 (Direct Cost: ¥1,700,000)
Keywordsmethyltransferase / repair enzyme / PCR / cDNA / X-irradiation
Research Abstract

An isoaspartyl protein carboxyl methyltransferase (PIMT) catalyzes the methyl esterification of D-aspartyl and L-isoaspartyl residues in proteins and peptides, converts the altered residues to normal L-aspartic acid residues, and is presumed to function in the repair of damaged proteins by racemization and deamidation. We have reported that the PIMT gene is expressed mainly in the brain and testis and have determined these cDNA sequences. We have also proved that the mRNA levels in cataractous lens epithelia are significantly lower than those in normal age-matched lens tissue. It is postulated that low dose X-irradiation facilitates the proliferation and prolongs the survival rate of the cells in vivo and in vitro. The physiological benefit from low level ionizing radiation is called as radiation hormesis. In this study, we examined the change of the PIMT mRNA levels in rat cultured fibroblast cells stimulated by irradiation to clarify the mechanism of the hormesis effect. We compared the PIMT gene expression in the cultured cells after 0, 25, 50 and 75 cGy irradiation. Three days after 50 and 75 cGy irradiation, PIMT mRNA levels increased about 1.5 and 2 times, respectively. These results suggest that the PIMT might play an important role in the hormesis mechanism.

Report

(3 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • Research Products

    (4 results)

All Other

All Publications (4 results)

  • [Publications] T.Doi,R.Takeda,et al.: "The expression of an isoaspartyl protein carboxyl methyltransferase gene in rat cultured fibroblast cells induced by low dose X-irradiation." Neurochemical Research. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] T.Doi, R.Takeda, Y.Nakashiro and M.Mizobuchi: "The Expression of an Isoaspartyl Protein Carboxyl Methyltransferase Gene in Rat Cultured Fibroblast Cells Induced by Low Dose X-Irradiation." Neurochemical Resaerch. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] T.Doi,Takeda,et al.: "The expression of an isoaspartyl protein carboxyl methyltransferase gene in rat cultured fibroblast cells induced by low dose X-irradiation." Neurochemical Research. (in press).

    • Related Report
      1998 Annual Research Report
  • [Publications] T.Doi, R.Takeda, et al.: "The expression of an isoaspartyl protein cazrboxyl methyltransferase gene in rat cultured fibroblast cells induced by low dose X-irradiation." Neurochemical Research. (in press).

    • Related Report
      1997 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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